Luteoprotective roles of luteinizing hormone are mediated by not only progesterone production but also glucocorticoid conversion in bovine corpus luteum

Syota Kawaguchi, Anom Bowolaksono, Ryosuke Sakumoto, Kiyoshi Okuda

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Luteinizing hormone (LH) is known as a key regulator of corpus luteum (CL) function, but the luteoprotective mechanisms of LH in the maintenance of bovine CL function are not well understood. The current study investigated if LH increases cell viability and induces cortisol conversion, and if the luteoprotective action of LH is mediated by stimulating the local production and action of progesterone (P4) and/or cortisol. Cultured bovine luteal cells obtained at the mid-luteal stage (Days 8-12 of the estrous cycle) were treated for 24hr with LH (10ng/ml) with/without onapristone (OP, a specific P4 receptor antagonist; 100μM), cortisone (1μM), and aminoglutethimide (AGT, a specific inhibitor of cytochrome P450 side-chain cleavage; 100μM). LH with and without OP significantly increased the mRNA and protein expressions of 11β-hydroxysteroid dehydrogenase (HSD11B) 1, but did not affect the mRNA or protein expression of HSD11B2. These treatments also significantly increased HSD11B1 activity. Cell viability was significantly increased by LH alone or by LH in combination with cortisone and OP. LH in combination with OP or AGT significantly decreased cell viability as compared to LH alone. The overall results suggest that LH stimulates not only P4 production but also HSD11B1 expression, thereby increasing the cortisol concentration in the bovine CL, and that LH prevents cell death through these survival pathways. LH may consequently support CL function during the luteal phase in cattle.

Original languageEnglish
Pages (from-to)204-211
Number of pages8
JournalMolecular Reproduction and Development
Volume80
Issue number3
DOIs
Publication statusPublished - Mar 1 2013

Keywords

  • Apoptosis
  • Corpus luteum
  • Cortisol
  • Luteinizing hormone
  • Progesterone

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Luteoprotective roles of luteinizing hormone are mediated by not only progesterone production but also glucocorticoid conversion in bovine corpus luteum'. Together they form a unique fingerprint.

  • Cite this