Abstract
Luteinizing hormone (LH) is known as a key regulator of corpus luteum (CL) function, but the luteoprotective mechanisms of LH in the maintenance of bovine CL function are not well understood. The current study investigated if LH increases cell viability and induces cortisol conversion, and if the luteoprotective action of LH is mediated by stimulating the local production and action of progesterone (P4) and/or cortisol. Cultured bovine luteal cells obtained at the mid-luteal stage (Days 8-12 of the estrous cycle) were treated for 24hr with LH (10ng/ml) with/without onapristone (OP, a specific P4 receptor antagonist; 100μM), cortisone (1μM), and aminoglutethimide (AGT, a specific inhibitor of cytochrome P450 side-chain cleavage; 100μM). LH with and without OP significantly increased the mRNA and protein expressions of 11β-hydroxysteroid dehydrogenase (HSD11B) 1, but did not affect the mRNA or protein expression of HSD11B2. These treatments also significantly increased HSD11B1 activity. Cell viability was significantly increased by LH alone or by LH in combination with cortisone and OP. LH in combination with OP or AGT significantly decreased cell viability as compared to LH alone. The overall results suggest that LH stimulates not only P4 production but also HSD11B1 expression, thereby increasing the cortisol concentration in the bovine CL, and that LH prevents cell death through these survival pathways. LH may consequently support CL function during the luteal phase in cattle.
| Original language | English |
|---|---|
| Pages (from-to) | 204-211 |
| Number of pages | 8 |
| Journal | Molecular Reproduction and Development |
| Volume | 80 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 2013 |
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Keywords
- Apoptosis
- Corpus luteum
- Cortisol
- Luteinizing hormone
- Progesterone
ASJC Scopus subject areas
- Genetics
- Developmental Biology
- Cell Biology
Cite this
Luteoprotective roles of luteinizing hormone are mediated by not only progesterone production but also glucocorticoid conversion in bovine corpus luteum. / Kawaguchi, Syota; Bowolaksono, Anom; Sakumoto, Ryosuke; Okuda, Kiyoshi.
In: Molecular Reproduction and Development, Vol. 80, No. 3, 03.2013, p. 204-211.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Luteoprotective roles of luteinizing hormone are mediated by not only progesterone production but also glucocorticoid conversion in bovine corpus luteum
AU - Kawaguchi, Syota
AU - Bowolaksono, Anom
AU - Sakumoto, Ryosuke
AU - Okuda, Kiyoshi
PY - 2013/3
Y1 - 2013/3
N2 - Luteinizing hormone (LH) is known as a key regulator of corpus luteum (CL) function, but the luteoprotective mechanisms of LH in the maintenance of bovine CL function are not well understood. The current study investigated if LH increases cell viability and induces cortisol conversion, and if the luteoprotective action of LH is mediated by stimulating the local production and action of progesterone (P4) and/or cortisol. Cultured bovine luteal cells obtained at the mid-luteal stage (Days 8-12 of the estrous cycle) were treated for 24hr with LH (10ng/ml) with/without onapristone (OP, a specific P4 receptor antagonist; 100μM), cortisone (1μM), and aminoglutethimide (AGT, a specific inhibitor of cytochrome P450 side-chain cleavage; 100μM). LH with and without OP significantly increased the mRNA and protein expressions of 11β-hydroxysteroid dehydrogenase (HSD11B) 1, but did not affect the mRNA or protein expression of HSD11B2. These treatments also significantly increased HSD11B1 activity. Cell viability was significantly increased by LH alone or by LH in combination with cortisone and OP. LH in combination with OP or AGT significantly decreased cell viability as compared to LH alone. The overall results suggest that LH stimulates not only P4 production but also HSD11B1 expression, thereby increasing the cortisol concentration in the bovine CL, and that LH prevents cell death through these survival pathways. LH may consequently support CL function during the luteal phase in cattle.
AB - Luteinizing hormone (LH) is known as a key regulator of corpus luteum (CL) function, but the luteoprotective mechanisms of LH in the maintenance of bovine CL function are not well understood. The current study investigated if LH increases cell viability and induces cortisol conversion, and if the luteoprotective action of LH is mediated by stimulating the local production and action of progesterone (P4) and/or cortisol. Cultured bovine luteal cells obtained at the mid-luteal stage (Days 8-12 of the estrous cycle) were treated for 24hr with LH (10ng/ml) with/without onapristone (OP, a specific P4 receptor antagonist; 100μM), cortisone (1μM), and aminoglutethimide (AGT, a specific inhibitor of cytochrome P450 side-chain cleavage; 100μM). LH with and without OP significantly increased the mRNA and protein expressions of 11β-hydroxysteroid dehydrogenase (HSD11B) 1, but did not affect the mRNA or protein expression of HSD11B2. These treatments also significantly increased HSD11B1 activity. Cell viability was significantly increased by LH alone or by LH in combination with cortisone and OP. LH in combination with OP or AGT significantly decreased cell viability as compared to LH alone. The overall results suggest that LH stimulates not only P4 production but also HSD11B1 expression, thereby increasing the cortisol concentration in the bovine CL, and that LH prevents cell death through these survival pathways. LH may consequently support CL function during the luteal phase in cattle.
KW - Apoptosis
KW - Corpus luteum
KW - Cortisol
KW - Luteinizing hormone
KW - Progesterone
UR - http://www.scopus.com/inward/record.url?scp=84875525055&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84875525055&partnerID=8YFLogxK
U2 - 10.1002/mrd.22150
DO - 10.1002/mrd.22150
M3 - Article
C2 - 23325624
AN - SCOPUS:84875525055
VL - 80
SP - 204
EP - 211
JO - Molecular Reproduction and Development
JF - Molecular Reproduction and Development
SN - 1040-452X
IS - 3
ER -