Luteolin overcomes resistance to benzyl isothiocyanate-induced apoptosis in human colorectal cancer HCT-116 cells

Rieko Sakai; Shintaro Yokobe; Naomi Abe; Noriyuki Miyoshi; Yoshiyuki Murata; Yoshimasa Nakamura

Luteolin overcomes resistance to benzyl isothiocyanate-induced apoptosis in human colorectal cancer HCT-116 cells

Rieko Sakai, Shintaro Yokobe, Naomi Abe, Noriyuki Miyoshi, Yoshiyuki Murata, Yoshimasa Nakamura

Research output: Contribution to journal › Article › peer-review

Abstract

We have previously identified p53, a universal sensor of genotoxic stress, as a negative regulator of the apoptosis induction by benzyl isothiocyanate (BITC) in the normal colon fibroblastoid cells. In the present study, we further confirmed that BITC has a potential to induce cytotoxicity in the p53-mutated colon cancer HT-29 cells in preference to HCT-116 cells with wild-type p53. To obtain effective induction of BITC-stimulated apoptosis in p53-positive cells, we investigated the combination effect of BITC and food ingredient that may overcome resistance to BITC. Pretreatment with luteolin potentiated the cytotoxicity induction by BITC in HCT-116 cells but not in HT-29 cells. The biochemical events related to apoptosis such as DNA ladder formation and caspase-3 activation were also enhanced by luteolin. Luteolin attenuated the expression of p21^waf1/cip1, a key downstream target of p53. These results suggest the role of p21^waf1/cip1 pathway in the overcoming BITC resistance by luteolin.

Original language	English
Pages (from-to)	389-393
Number of pages	5
Journal	Journal of Food and Drug Analysis
Volume	20
Issue number	SUPPL.1
Publication status	Published - Apr 1 2012

Keywords

Apoptosis
Benzyl isothiocyanate
Caspase-3
HCT116 cells
HT-29 cells
Luteolin
P21

ASJC Scopus subject areas

Food Science
Pharmacology

Fingerprint Dive into the research topics of 'Luteolin overcomes resistance to benzyl isothiocyanate-induced apoptosis in human colorectal cancer HCT-116 cells'. Together they form a unique fingerprint.

Cite this

Luteolin overcomes resistance to benzyl isothiocyanate-induced apoptosis in human colorectal cancer HCT-116 cells. / Sakai, Rieko; Yokobe, Shintaro; Abe, Naomi; Miyoshi, Noriyuki; Murata, Yoshiyuki ; Nakamura, Yoshimasa.

In: Journal of Food and Drug Analysis, Vol. 20, No. SUPPL.1, 01.04.2012, p. 389-393.

Research output: Contribution to journal › Article › peer-review

@article{fd28ad4cf0d342378c8534f44160ac0e,

title = "Luteolin overcomes resistance to benzyl isothiocyanate-induced apoptosis in human colorectal cancer HCT-116 cells",

abstract = "We have previously identified p53, a universal sensor of genotoxic stress, as a negative regulator of the apoptosis induction by benzyl isothiocyanate (BITC) in the normal colon fibroblastoid cells. In the present study, we further confirmed that BITC has a potential to induce cytotoxicity in the p53-mutated colon cancer HT-29 cells in preference to HCT-116 cells with wild-type p53. To obtain effective induction of BITC-stimulated apoptosis in p53-positive cells, we investigated the combination effect of BITC and food ingredient that may overcome resistance to BITC. Pretreatment with luteolin potentiated the cytotoxicity induction by BITC in HCT-116 cells but not in HT-29 cells. The biochemical events related to apoptosis such as DNA ladder formation and caspase-3 activation were also enhanced by luteolin. Luteolin attenuated the expression of p21waf1/cip1, a key downstream target of p53. These results suggest the role of p21waf1/cip1 pathway in the overcoming BITC resistance by luteolin.",

keywords = "Apoptosis, Benzyl isothiocyanate, Caspase-3, HCT116 cells, HT-29 cells, Luteolin, P21",

author = "Rieko Sakai and Shintaro Yokobe and Naomi Abe and Noriyuki Miyoshi and Yoshiyuki Murata and Yoshimasa Nakamura",

year = "2012",

month = apr,

day = "1",

language = "English",

volume = "20",

pages = "389--393",

journal = "Journal of Food and Drug Analysis",

issn = "1021-9498",

publisher = "National Laboratories of Foods and Drugs",

number = "SUPPL.1",

}

TY - JOUR

T1 - Luteolin overcomes resistance to benzyl isothiocyanate-induced apoptosis in human colorectal cancer HCT-116 cells

AU - Sakai, Rieko

AU - Yokobe, Shintaro

AU - Abe, Naomi

AU - Miyoshi, Noriyuki

AU - Murata, Yoshiyuki

AU - Nakamura, Yoshimasa

PY - 2012/4/1

Y1 - 2012/4/1

N2 - We have previously identified p53, a universal sensor of genotoxic stress, as a negative regulator of the apoptosis induction by benzyl isothiocyanate (BITC) in the normal colon fibroblastoid cells. In the present study, we further confirmed that BITC has a potential to induce cytotoxicity in the p53-mutated colon cancer HT-29 cells in preference to HCT-116 cells with wild-type p53. To obtain effective induction of BITC-stimulated apoptosis in p53-positive cells, we investigated the combination effect of BITC and food ingredient that may overcome resistance to BITC. Pretreatment with luteolin potentiated the cytotoxicity induction by BITC in HCT-116 cells but not in HT-29 cells. The biochemical events related to apoptosis such as DNA ladder formation and caspase-3 activation were also enhanced by luteolin. Luteolin attenuated the expression of p21waf1/cip1, a key downstream target of p53. These results suggest the role of p21waf1/cip1 pathway in the overcoming BITC resistance by luteolin.

AB - We have previously identified p53, a universal sensor of genotoxic stress, as a negative regulator of the apoptosis induction by benzyl isothiocyanate (BITC) in the normal colon fibroblastoid cells. In the present study, we further confirmed that BITC has a potential to induce cytotoxicity in the p53-mutated colon cancer HT-29 cells in preference to HCT-116 cells with wild-type p53. To obtain effective induction of BITC-stimulated apoptosis in p53-positive cells, we investigated the combination effect of BITC and food ingredient that may overcome resistance to BITC. Pretreatment with luteolin potentiated the cytotoxicity induction by BITC in HCT-116 cells but not in HT-29 cells. The biochemical events related to apoptosis such as DNA ladder formation and caspase-3 activation were also enhanced by luteolin. Luteolin attenuated the expression of p21waf1/cip1, a key downstream target of p53. These results suggest the role of p21waf1/cip1 pathway in the overcoming BITC resistance by luteolin.

KW - Apoptosis

KW - Benzyl isothiocyanate

KW - Caspase-3

KW - HCT116 cells

KW - HT-29 cells

KW - Luteolin

KW - P21

UR - http://www.scopus.com/inward/record.url?scp=84863658160&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863658160&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84863658160

VL - 20

SP - 389

EP - 393

JO - Journal of Food and Drug Analysis

JF - Journal of Food and Drug Analysis

SN - 1021-9498

IS - SUPPL.1

ER -

Luteolin overcomes resistance to benzyl isothiocyanate-induced apoptosis in human colorectal cancer HCT-116 cells

Abstract

Keywords

ASJC Scopus subject areas

Other files and links

Cite this