Luteinizing hormone receptors in the bovine corpus luteum during the oestrous cycle and pregnancy

K. Okuda, Y. Uenoyama, C. Naito, Y. Sakabe, N. Kawate

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The concentration and affinity of luteinizing hormone (LH) receptors in bovine luteal tissues during the oestrous cycle and pregnancy were investigated by Scatchard analysis of the binding of 125I-labeled human chorionic gonadotropin. Corpora lutea (CL) were classified into five stages of the oestrous cycle and three stages of pregnancy. The concentration of LH receptors sharply increased from the early I stage of the oestrous cycle (Days 2-3; 3.09 fmol mg-1 protein) to the early II stage (Days 5-6; 9.44 fmol mg-1 protein) and then remained constant until the late luteal stage (Days 15-17; 8.14-9.56 fmol mg-1 protein). The LH receptors could not be analysed in the regressed luteal tissue due to the small amounts of binding. There was no significant difference in the concentrations of LH receptors (5.63-9.64 fmol mg-1 protein) among the three stages of pregnancy. Moreover, the concentrations of the receptors in the CL of pregnancy were comparable to those in the mid-cycle CL. The binding affinity did not change significantly during the oestrous cycle and pregnancy. Based on these results, it is assumed that the luteal function during the entire period of pregnancy might be regulated, at least in part, by LH, which is mediated via its specific receptors, and that the luteal function during pregnancy seems not to be regulated by changes in the binding capacity and affinity of LH receptors. To understand the physiological roles of LH in regulating luteal function in pregnant cows, further studies are required.

Original languageEnglish
Pages (from-to)147-151
Number of pages5
JournalReproduction, Fertility and Development
Volume11
Issue number3
Publication statusPublished - 1999

Fingerprint

LH Receptors
Corpus Luteum
corpus luteum
estrous cycle
pregnancy
Pregnancy
cattle
luteinizing hormone
Luteinizing Hormone
Proteins
proteins
luteinizing hormone receptors
receptors
human chorionic gonadotropin
binding capacity
Chorionic Gonadotropin
cows

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Reproductive Medicine
  • Developmental Biology
  • Endocrinology

Cite this

Luteinizing hormone receptors in the bovine corpus luteum during the oestrous cycle and pregnancy. / Okuda, K.; Uenoyama, Y.; Naito, C.; Sakabe, Y.; Kawate, N.

In: Reproduction, Fertility and Development, Vol. 11, No. 3, 1999, p. 147-151.

Research output: Contribution to journalArticle

Okuda, K, Uenoyama, Y, Naito, C, Sakabe, Y & Kawate, N 1999, 'Luteinizing hormone receptors in the bovine corpus luteum during the oestrous cycle and pregnancy', Reproduction, Fertility and Development, vol. 11, no. 3, pp. 147-151.
Okuda, K. ; Uenoyama, Y. ; Naito, C. ; Sakabe, Y. ; Kawate, N. / Luteinizing hormone receptors in the bovine corpus luteum during the oestrous cycle and pregnancy. In: Reproduction, Fertility and Development. 1999 ; Vol. 11, No. 3. pp. 147-151.
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