Lowfat, a mammalian Lix1 homologue, regulates leg size and growth under the Dachsous/Fat signaling pathway during tissue regeneration†

Tetsuya Bando, Yoshimasa Hamada, Kazuki Kurita, Taro Nakamura, Taro Mito, Hideyo Ohuchi, Sumihare Noji

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In the cricket Gryllus bimaculatus, missing distal parts of amputated legs are regenerated from blastemas based on positional information. The Dachsous/Fat (Ds/Ft) signaling pathway regulates blastema cell proliferation and positional information along the longitudinal axis during leg regeneration. Herein, we show that the Gryllus homologue of Lowfat (Gb'Lft), which modulates Ds/Ft signaling in Drosophila, is involved in leg regeneration. Gb'lft is expressed in regenerating legs, and RNAi against Gb'lft (Gb'lftRNAi) suppressed blastema cell hyperproliferation caused by Gb'ftRNAi or Gb'dsRNAi but enhanced that caused by Gb'kibraRNAi or Gb'wartsRNAi. In Gb'lftRNAi nymphs, missing parts of amputated legs were regenerated, but the length of the regenerated legs was shortened depending on the position of the amputation. Both normal and reversed intercalary regeneration occurred in Gb'lftRNAi nymphs, suggesting that Gb'Lft is involved in blastema cell proliferation and longitudinal leg regeneration under the Ds/Ft signaling pathway, but it is not required for intercalary regeneration.

Original languageEnglish
Pages (from-to)1440-1453
Number of pages14
JournalDevelopmental Dynamics
Volume240
Issue number6
DOIs
Publication statusPublished - Jun 2011
Externally publishedYes

Fingerprint

Regeneration
Leg
Fats
Growth
Nymph
Cell Proliferation
Gryllidae
RNA Interference
Amputation
Drosophila

Keywords

  • Dachsous/Fat signaling
  • Gryllus bimaculatus
  • Regeneration

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Lowfat, a mammalian Lix1 homologue, regulates leg size and growth under the Dachsous/Fat signaling pathway during tissue regeneration†. / Bando, Tetsuya; Hamada, Yoshimasa; Kurita, Kazuki; Nakamura, Taro; Mito, Taro; Ohuchi, Hideyo; Noji, Sumihare.

In: Developmental Dynamics, Vol. 240, No. 6, 06.2011, p. 1440-1453.

Research output: Contribution to journalArticle

Bando, Tetsuya ; Hamada, Yoshimasa ; Kurita, Kazuki ; Nakamura, Taro ; Mito, Taro ; Ohuchi, Hideyo ; Noji, Sumihare. / Lowfat, a mammalian Lix1 homologue, regulates leg size and growth under the Dachsous/Fat signaling pathway during tissue regeneration†. In: Developmental Dynamics. 2011 ; Vol. 240, No. 6. pp. 1440-1453.
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