Abstract
Fruit ripening in response to treatments with propylene, 1-methycyclopropene (1-MCP), and low temperature was characterized in 'Sanuki Gold' kiwifruit, Actinidia chinensis Planch. Propylene treatment immediately induced rapid fruit softening, increased AC-PG (polygalacturonase) and AC-EXP (expansin) mRNA accumulation, and stimulated an increase in the soluble solid concentration (SSC) and a decrease in titratable acidity (TA). After 3d exposure to propylene, ethylene production and AC-PL (pectate lyase) mRNA accumulation were observed. 1-MCP treatment after 24h exposure to propylene eliminated AC-PG mRNA accumulation and suppressed continued changes in SSC and TA. Application of 1-MCP at the start of the treatment, followed by continuous propylene exposure, markedly delayed fruit softening, and the expression of the cell wall-modifying genes, and changes in the SSC and TA, indicating that kiwifruit become insensitive to ethylene at least for 3d following 1-MCP exposure. Surprisingly, significant fruit softening, mRNA accumulation of AC-PG, AC-PL, and AC-EXP, and decreased TA were observed without ethylene production in intact fruit stored at low temperature for 1 month, but not in fruit stored at room temperature. Repeated 1-MCP treatments (twice a week) failed to inhibit the changes that occurred in low temperature storage. These observations indicate that low temperature modulates the ripening of kiwifruit in an ethylene-independent manner, suggesting that kiwifruit ripening is inducible by either ethylene or low temperature signals.
| Original language | English |
|---|---|
| Pages (from-to) | 963-971 |
| Number of pages | 9 |
| Journal | Journal of Experimental Botany |
| Volume | 63 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jan 2012 |
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Keywords
- 1-MCP
- Ethylene
- fruit ripening
- kiwifruit
- low-temperature
ASJC Scopus subject areas
- Plant Science
- Physiology
Cite this
Low-temperature-modulated fruit ripening is independent of ethylene in 'Sanuki Gold' kiwifruit. / Mworia, Eric G.; Yoshikawa, Takashi; Salikon, Nadiah; Oda, Chisato; Asiche, William O.; Yokotani, Naoki; Abe, Daigo; Ushijima, Koichiro; Nakano, Ryohei; Kubo, Yasutaka.
In: Journal of Experimental Botany, Vol. 63, No. 2, 01.2012, p. 963-971.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Low-temperature-modulated fruit ripening is independent of ethylene in 'Sanuki Gold' kiwifruit
AU - Mworia, Eric G.
AU - Yoshikawa, Takashi
AU - Salikon, Nadiah
AU - Oda, Chisato
AU - Asiche, William O.
AU - Yokotani, Naoki
AU - Abe, Daigo
AU - Ushijima, Koichiro
AU - Nakano, Ryohei
AU - Kubo, Yasutaka
PY - 2012/1
Y1 - 2012/1
N2 - Fruit ripening in response to treatments with propylene, 1-methycyclopropene (1-MCP), and low temperature was characterized in 'Sanuki Gold' kiwifruit, Actinidia chinensis Planch. Propylene treatment immediately induced rapid fruit softening, increased AC-PG (polygalacturonase) and AC-EXP (expansin) mRNA accumulation, and stimulated an increase in the soluble solid concentration (SSC) and a decrease in titratable acidity (TA). After 3d exposure to propylene, ethylene production and AC-PL (pectate lyase) mRNA accumulation were observed. 1-MCP treatment after 24h exposure to propylene eliminated AC-PG mRNA accumulation and suppressed continued changes in SSC and TA. Application of 1-MCP at the start of the treatment, followed by continuous propylene exposure, markedly delayed fruit softening, and the expression of the cell wall-modifying genes, and changes in the SSC and TA, indicating that kiwifruit become insensitive to ethylene at least for 3d following 1-MCP exposure. Surprisingly, significant fruit softening, mRNA accumulation of AC-PG, AC-PL, and AC-EXP, and decreased TA were observed without ethylene production in intact fruit stored at low temperature for 1 month, but not in fruit stored at room temperature. Repeated 1-MCP treatments (twice a week) failed to inhibit the changes that occurred in low temperature storage. These observations indicate that low temperature modulates the ripening of kiwifruit in an ethylene-independent manner, suggesting that kiwifruit ripening is inducible by either ethylene or low temperature signals.
AB - Fruit ripening in response to treatments with propylene, 1-methycyclopropene (1-MCP), and low temperature was characterized in 'Sanuki Gold' kiwifruit, Actinidia chinensis Planch. Propylene treatment immediately induced rapid fruit softening, increased AC-PG (polygalacturonase) and AC-EXP (expansin) mRNA accumulation, and stimulated an increase in the soluble solid concentration (SSC) and a decrease in titratable acidity (TA). After 3d exposure to propylene, ethylene production and AC-PL (pectate lyase) mRNA accumulation were observed. 1-MCP treatment after 24h exposure to propylene eliminated AC-PG mRNA accumulation and suppressed continued changes in SSC and TA. Application of 1-MCP at the start of the treatment, followed by continuous propylene exposure, markedly delayed fruit softening, and the expression of the cell wall-modifying genes, and changes in the SSC and TA, indicating that kiwifruit become insensitive to ethylene at least for 3d following 1-MCP exposure. Surprisingly, significant fruit softening, mRNA accumulation of AC-PG, AC-PL, and AC-EXP, and decreased TA were observed without ethylene production in intact fruit stored at low temperature for 1 month, but not in fruit stored at room temperature. Repeated 1-MCP treatments (twice a week) failed to inhibit the changes that occurred in low temperature storage. These observations indicate that low temperature modulates the ripening of kiwifruit in an ethylene-independent manner, suggesting that kiwifruit ripening is inducible by either ethylene or low temperature signals.
KW - 1-MCP
KW - Ethylene
KW - fruit ripening
KW - kiwifruit
KW - low-temperature
UR - http://www.scopus.com/inward/record.url?scp=84855897055&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84855897055&partnerID=8YFLogxK
U2 - 10.1093/jxb/err324
DO - 10.1093/jxb/err324
M3 - Article
C2 - 22058408
AN - SCOPUS:84855897055
VL - 63
SP - 963
EP - 971
JO - Journal of Experimental Botany
JF - Journal of Experimental Botany
SN - 0022-0957
IS - 2
ER -