Low-dose exogenous interleukin (IL)-12 enhances antigen-induced interferon-γ production without affecting IL-10 production in asthmatics

M. Horiba, G. Kimura, Y. Tanimoto, A. Kanehiro, A. Takeda, C. Okada, M. Kataoka, K. Takahashi, M. Harada

Research output: Contribution to journalArticle

Abstract

Background: Although interleukin (IL)-12, IL-10 and interferon (IFN)-γ are key cytokines that control the T helper (Th) 1/Th2 balance in human allergic disorders, details of their interactions in humans have not been clarified. Recently IL-10, one of the Th2 cytokines, has been shown to have an anti-inflammatory effect against allergic responses. To clarify the effect of IL-12 on the production of IFN-γ and IL-10, in the present study we examined responses of peripheral blood mononuclear cells (PBMC) from asthmatics to stimulation by Dermatophagoides farinae (Df) antigen in the presence of exogenous IL-12. Methods: Peripheral blood mononuclear cells of Df-sensitized (n = 7) and non-sensitized (n = 5) asthmatics were stimulated by Df antigen after incubation with exogenous IL-12 (100 pg/mL). Interferon-γ and IL-10 produced in culture supernatants were measured by ELISA. The effect of IL-12 on lymphocyte proliferation was assayed by [3H]-thymidine incorporation in both groups. Results: The production of IFN-γ by PBMC was significantly enhanced by incubation with IL-12 in both patient groups (P <0.05), while IL-12 did not affect the production of IL-10 in either group. Lymphocyte proliferation induced by Df antigen was significantly higher in the Df-sensitized group (P <0.01). This lymphocyte proliferation was significantly enhanced by exogenous IL-12 only in the Df-sensitized group (P <0.05). Conclusions: These observations indicate that IL-12 enhances Th1-shifted immune responses without affecting IL-10 production and suggest that IL-12 may effectively inhibit the Th2 dominant state of bronchial asthma by regulating the Th1/Th2 balance.

Original languageEnglish
Pages (from-to)143-151
Number of pages9
JournalAllergology International
Volume50
Issue number2
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Interleukin-12
Interleukin-10
Interferons
Antigens
Dermatophagoides farinae
Dermatophagoides Antigens
Blood Cells
Lymphocytes
Th1-Th2 Balance
Cytokines
Thymidine
Anti-Inflammatory Agents
Asthma
Enzyme-Linked Immunosorbent Assay

Keywords

  • Bronchial asthma
  • Dermatophagoides farinae
  • Interferon-γ
  • Interleukin-10
  • Interleukin-12
  • Peripheral blood mononuclear cells

ASJC Scopus subject areas

  • Immunology and Allergy

Cite this

Low-dose exogenous interleukin (IL)-12 enhances antigen-induced interferon-γ production without affecting IL-10 production in asthmatics. / Horiba, M.; Kimura, G.; Tanimoto, Y.; Kanehiro, A.; Takeda, A.; Okada, C.; Kataoka, M.; Takahashi, K.; Harada, M.

In: Allergology International, Vol. 50, No. 2, 2001, p. 143-151.

Research output: Contribution to journalArticle

Horiba, M, Kimura, G, Tanimoto, Y, Kanehiro, A, Takeda, A, Okada, C, Kataoka, M, Takahashi, K & Harada, M 2001, 'Low-dose exogenous interleukin (IL)-12 enhances antigen-induced interferon-γ production without affecting IL-10 production in asthmatics', Allergology International, vol. 50, no. 2, pp. 143-151. https://doi.org/10.1046/j.1440-1592.2001.00214.x
Horiba, M. ; Kimura, G. ; Tanimoto, Y. ; Kanehiro, A. ; Takeda, A. ; Okada, C. ; Kataoka, M. ; Takahashi, K. ; Harada, M. / Low-dose exogenous interleukin (IL)-12 enhances antigen-induced interferon-γ production without affecting IL-10 production in asthmatics. In: Allergology International. 2001 ; Vol. 50, No. 2. pp. 143-151.
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AU - Horiba, M.

AU - Kimura, G.

AU - Tanimoto, Y.

AU - Kanehiro, A.

AU - Takeda, A.

AU - Okada, C.

AU - Kataoka, M.

AU - Takahashi, K.

AU - Harada, M.

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AB - Background: Although interleukin (IL)-12, IL-10 and interferon (IFN)-γ are key cytokines that control the T helper (Th) 1/Th2 balance in human allergic disorders, details of their interactions in humans have not been clarified. Recently IL-10, one of the Th2 cytokines, has been shown to have an anti-inflammatory effect against allergic responses. To clarify the effect of IL-12 on the production of IFN-γ and IL-10, in the present study we examined responses of peripheral blood mononuclear cells (PBMC) from asthmatics to stimulation by Dermatophagoides farinae (Df) antigen in the presence of exogenous IL-12. Methods: Peripheral blood mononuclear cells of Df-sensitized (n = 7) and non-sensitized (n = 5) asthmatics were stimulated by Df antigen after incubation with exogenous IL-12 (100 pg/mL). Interferon-γ and IL-10 produced in culture supernatants were measured by ELISA. The effect of IL-12 on lymphocyte proliferation was assayed by [3H]-thymidine incorporation in both groups. Results: The production of IFN-γ by PBMC was significantly enhanced by incubation with IL-12 in both patient groups (P <0.05), while IL-12 did not affect the production of IL-10 in either group. Lymphocyte proliferation induced by Df antigen was significantly higher in the Df-sensitized group (P <0.01). This lymphocyte proliferation was significantly enhanced by exogenous IL-12 only in the Df-sensitized group (P <0.05). Conclusions: These observations indicate that IL-12 enhances Th1-shifted immune responses without affecting IL-10 production and suggest that IL-12 may effectively inhibit the Th2 dominant state of bronchial asthma by regulating the Th1/Th2 balance.

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KW - Dermatophagoides farinae

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KW - Interleukin-12

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