Low-dose γ-ray irradiation reduces oxidative damage induced by CCl4 in mouse liver

Takaharu Nomura, Kiyonori Yamaoka

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

We examined the effects of irradiation (50 cGy of γ-ray) reducing the oxidative damage in carbon tetrachloride (CCl4)-hepatopathy mice. We made pathological examinations and analyzed transaminase activity (glutamic oxaloacetic transaminase and glutamic pyruvic transaminase), lipid peroxide level and the activities of endogenous antioxidants in the mouse. The irradiation was found to accelerate the recovery. Based on pathological examination as well as changes in each transaminase activity and lipid peroxide levels, it was shown that hepatopathy improved 3 d after the irradiation. The activities of glutathione reductase and glutathione peroxidase rapidly elevated after irradiation, and the total glutathione content gradually increased in the irradiation group. Both activities of γ-glutamylcysteine synthetase and catalase were higher than normal at all times after the irradiation and gradually increased. In addition, the γ-glutamylcysteine synthetase activity changed in a similar fashion to the total glutathione content. However, superoxide dismutase activity in both groups decreased and that of the irradiation group was significantly lower than that of the sham-irradiation group. These findings suggest that low-dose radiation relieved functional disorder at least in the liver of mice with active oxygen diseases. Copyright (C) 1999 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)1324-1333
Number of pages10
JournalFree Radical Biology and Medicine
Volume27
Issue number11-12
DOIs
Publication statusPublished - 1999

Fingerprint

Liver
Glutamate-Cysteine Ligase
Dosimetry
Lipid Peroxides
Irradiation
Transaminases
Glutathione
Glutathione Reductase
Carbon Tetrachloride
Glutathione Peroxidase
Aspartate Aminotransferases
Alanine Transaminase
Catalase
Superoxide Dismutase
Reactive Oxygen Species
Antioxidants
Radiation
Recovery

Keywords

  • γ-Ray irradiation
  • Antioxidant enzymes
  • Carbon tetrachloride
  • Free radical
  • Glutathione
  • Glutathione-related enzymes
  • Mouse liver

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Low-dose γ-ray irradiation reduces oxidative damage induced by CCl4 in mouse liver. / Nomura, Takaharu; Yamaoka, Kiyonori.

In: Free Radical Biology and Medicine, Vol. 27, No. 11-12, 1999, p. 1324-1333.

Research output: Contribution to journalArticle

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