Low density lipoprotein adsorption on sol-gel derived alumina for blood purification therapy

Takuji Asano, Kanji Tsuru, Satoshi Hayakawa, Akiyoshi Osaka

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Among the clinical treatments of Familial Hyper cholesterolemia patients to reduce the concentration of low density lipoprotein (LDL), blood purification therapy is most suitable in which a blood-compatible adsorbent is employed. In the present study, alumina powders were prepared via a sol-gel route to develop a LDL-adsorbent Aluminum tri2-propoxide was hydrolyzed and subsequently calcined up to 1200°C. Surface charge density and pore size distribution were measured, and the phases were identified. The alumina calcined above 400°C had excellent blood compatibility in terms of endogenous clotting parameters, i.e., partial thromboplastin time: (PTT), prothrombin time: (PT), and the amount of fibrinogen: (Fib). The amount of LDL-adsorption (ΔWLDL) increased with the calcining temperature, showing a good linear correlation to surface charge density. The 1200°C sample consisted only of α-alumina, and was greatest in ΔWLDL. All samples involved pores smaller than 20 nm but not the pores large enough to accommodate LDL molecules (20-25 nm). From those results, it was concluded for the present alumina particles that the surface charge density was the primary factor and that the chemical activity of α-alumina also contributed to the excellent LDL-adsorption for the 1200°C sample, while entrapping LDL in the pores was not an active mechanism.

Original languageEnglish
Pages (from-to)161-170
Number of pages10
JournalBio-Medical Materials and Engineering
Volume18
Issue number3
DOIs
Publication statusPublished - Sep 1 2008

Keywords

  • Adsorbents
  • Alumina
  • Charge density
  • Low density lipoprotein
  • Sol-gel method

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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