Loss of necrotic spotted lesions 1 associates with cell death and defense responses in Arabidopsis thaliana

Yoshiteru Noutoshi, Takashi Kuromori, Takuji Wada, Takashi Hirayama, Asako Kamiya, Yuko Imura, Michiko Yasuda, Hideo Nakashita, Ken Shirasu, Kazuo Shinozaki

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

We isolated a lesion mimic mutant, necrotic spotted lesions 1 (nsl1), from Ds-tagged Arabidopsis thaliana accession No-0. The nsl1 mutant exhibits a growth retardation phenotype and develops spotted necrotic lesions on its rosette and cauline leaves. These phenotypes occur in the absence of pathogens indicating that nsl1 mutants may constitutively express defense responses. Consistent with this idea, nsl1 accumulates high levels of callose and autofluorescent phenolic compounds localized to the necrotic lesions. Furthermore RNA gel blot analysis revealed that genes associated with disease resistance activation are upregulated in the nsl1 mutants and these plants contain elevated levels of salicylic acid (SA). Crossing nsl1 with an SA deficient mutant, eds16-1, revealed that the nsl1 lesions and growth retardation are dependent upon SA. The nsl1 phenotypes are not suppressed under either the rar1-10 or sgt1b-1 genetic background. NSL1 encodes a novel 612aa protein which contains a membrane-attack complex/perforin (MACPF) domain, which is conserved in bacteria, fungi, mammals and plants. The possible modes of action of NSL1 protein in negative regulation of cell death programs and defense responses are discussed.

Original languageEnglish
Pages (from-to)29-42
Number of pages14
JournalPlant Molecular Biology
Volume62
Issue number1-2
DOIs
Publication statusPublished - Sep 2006
Externally publishedYes

Fingerprint

lesions (plant)
Salicylic Acid
Cell death
Arabidopsis
cell death
Cell Death
Arabidopsis thaliana
Phenotype
Complement Membrane Attack Complex
Perforin
Disease Resistance
Mammals
Pathogens
Growth
Fungi
Bacteria
Proteins
mutants
Genes
Gels

Keywords

  • Cell death
  • Defense responses
  • MACPF domain
  • Salicylic acid

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Loss of necrotic spotted lesions 1 associates with cell death and defense responses in Arabidopsis thaliana. / Noutoshi, Yoshiteru; Kuromori, Takashi; Wada, Takuji; Hirayama, Takashi; Kamiya, Asako; Imura, Yuko; Yasuda, Michiko; Nakashita, Hideo; Shirasu, Ken; Shinozaki, Kazuo.

In: Plant Molecular Biology, Vol. 62, No. 1-2, 09.2006, p. 29-42.

Research output: Contribution to journalArticle

Noutoshi, Y, Kuromori, T, Wada, T, Hirayama, T, Kamiya, A, Imura, Y, Yasuda, M, Nakashita, H, Shirasu, K & Shinozaki, K 2006, 'Loss of necrotic spotted lesions 1 associates with cell death and defense responses in Arabidopsis thaliana', Plant Molecular Biology, vol. 62, no. 1-2, pp. 29-42. https://doi.org/10.1007/s11103-006-9001-6
Noutoshi, Yoshiteru ; Kuromori, Takashi ; Wada, Takuji ; Hirayama, Takashi ; Kamiya, Asako ; Imura, Yuko ; Yasuda, Michiko ; Nakashita, Hideo ; Shirasu, Ken ; Shinozaki, Kazuo. / Loss of necrotic spotted lesions 1 associates with cell death and defense responses in Arabidopsis thaliana. In: Plant Molecular Biology. 2006 ; Vol. 62, No. 1-2. pp. 29-42.
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