Loss-of-function mutations in three homoeologous PHYTOCLOCK 1 genes in common wheat are associated with the extra-early flowering phenotype

Nobuyuki Mizuno, Mika Kinoshita, Saki Kinoshita, Hidetaka Nishida, Masaya Fujita, Kenji Kato, Koji Murai, Shuhei Nasuda

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Abstract

Triticum aestivum L. cv 'Chogokuwase' is an extra-early flowering common wheat cultivar that is insensitive to photoperiod conferred by the photoperiod insensitive alleles at the Photoperiod-B1 (Ppd-B1) and Ppd-D1loci, and does not require vernalization for flowering. This reduced vernalization requirement is likely due to the spring habitat allele Vrn-D1 at the VERNALIZATION-D1 locus. Genotypes of the Ppd-1 loci that determine photoperiod sensitivity do not fully explain the insensitivity to photoperiod seen in 'Chogokuwase'. We detected altered expression patterns of clock and clock-output genes including Ppd-1 in 'Chogokuwase' that were similar to those in an einkorn wheat mutant that lacks the clockgene homologue, wheat PHYTOCLOCK 1 (WPCL1). Presumptive loss-of-function mutations in all WPCL1 homoeologous genes were found in 'Chogokuwase' and 'Geurumil', one of the parental cultivars. Segregation analysis of the two intervarietal F2 populations revealed that all the examined F2 plants that headed as early as 'Chogokuwase' had the loss-of-function wpcl1 alleles at all three homoeoloci. Some F2 plants carrying the wpcl1 alleles at three homoeoloci headed later than 'Chogokuwase', suggesting the presence of other loci influencing heading date. Flowering repressor Vrn-2 was up-regulated in 'Chogokuwase' and 'Geurumil' that had the triple recessive wpcl1 alleles. An elevated transcript abundance of Vrn-2 could explain the observation that 'Geurumil' and some F2 plants carrying the three recessive wpcl1 homeoealleles headed later than 'Chogokuwase'. In spite of the up-regulation of Vrn-2, 'Chogokuwase' may have headed earlier due to unidentified earliness genes. Our observations indicated that loss-of-function mutations in the clock gene wpcl1 are necessary but are not sufficient to explain the extra-early heading of 'Chogokuwase'.

Original languageEnglish
Article numbere0165618
JournalPLoS One
Volume11
Issue number10
DOIs
Publication statusPublished - Oct 1 2016

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Photoperiod
Triticum
photoperiod
Genes
Alleles
flowering
alleles
Phenotype
phenotype
Clocks
Mutation
wheat
vernalization
heading
genes
loci
Triticum monococcum subsp. monococcum
cultivars
Ecosystem
early development

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Loss-of-function mutations in three homoeologous PHYTOCLOCK 1 genes in common wheat are associated with the extra-early flowering phenotype. / Mizuno, Nobuyuki; Kinoshita, Mika; Kinoshita, Saki; Nishida, Hidetaka; Fujita, Masaya; Kato, Kenji; Murai, Koji; Nasuda, Shuhei.

In: PLoS One, Vol. 11, No. 10, e0165618, 01.10.2016.

Research output: Contribution to journalArticle

Mizuno, Nobuyuki ; Kinoshita, Mika ; Kinoshita, Saki ; Nishida, Hidetaka ; Fujita, Masaya ; Kato, Kenji ; Murai, Koji ; Nasuda, Shuhei. / Loss-of-function mutations in three homoeologous PHYTOCLOCK 1 genes in common wheat are associated with the extra-early flowering phenotype. In: PLoS One. 2016 ; Vol. 11, No. 10.
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AU - Kinoshita, Saki

AU - Nishida, Hidetaka

AU - Fujita, Masaya

AU - Kato, Kenji

AU - Murai, Koji

AU - Nasuda, Shuhei

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