Loss-of-function analyses of the fragile X-related and dopamine receptor genes by RNA interference in the cricket Gryllus bimaculatus

Aska Hamada, Katsuyuki Miyawaki, Eri Honda-sumi, Kenji Tomioka, Taro Mito, Hideyo Ohuchi, Sumihare Noji

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In order to explore a possibility that the cricket Gryllus bimaculatus would be a useful model to unveil molecular mechanisms of human diseases, we performed loss-of-function analyses of Gryllus genes homologous to human genes that are responsible for human disorders, fragile X mental retardation 1 (fmr1) and Dopamine receptor (DopR). We cloned cDNAs of their Gryllus homologues, Gb'fmr1, Gb'DopRI, and Gb'DopRII, and analyzed their functions with use of nymphal RNA interference (RNAi). For Gb'fmr1, three major phenotypes were observed: (1) abnormal wing postures, (2) abnormal calling song, and (3) loss of the circadian locomotor rhythm, while for Gb'DopRI, defects of wing posture and morphology were found. These results indicate that the cricket has the potential to become a novel model system to explore human neuronal pathogenic mechanisms and to screen therapeutic drugs by RNAi.

Original languageEnglish
Pages (from-to)2025-2033
Number of pages9
JournalDevelopmental Dynamics
Volume238
Issue number8
DOIs
Publication statusPublished - Aug 1 2008

Keywords

  • Calling song
  • Circadian locomotor rhythm
  • Cricket
  • Dopamine receptor
  • Fragile X mental retardation
  • RNA interference
  • Wing posture

ASJC Scopus subject areas

  • Developmental Biology

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