Loss-of-function analyses of the fragile X-related and dopamine receptor genes by RNA interference in the cricket Gryllus bimaculatus

Aska Hamada, Katsuyuki Miyawaki, Eri Honda-sumi, Kenji Tomioka, Taro Mito, Hideyo Ohuchi, Sumihare Noji

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In order to explore a possibility that the cricket Gryllus bimaculatus would be a useful model to unveil molecular mechanisms of human diseases, we performed loss-of-function analyses of Gryllus genes homologous to human genes that are responsible for human disorders, fragile X mental retardation 1 (fmr1) and Dopamine receptor (DopR). We cloned cDNAs of their Gryllus homologues, Gb'fmr1, Gb'DopRI, and Gb'DopRII, and analyzed their functions with use of nymphal RNA interference (RNAi). For Gb'fmr1, three major phenotypes were observed: (1) abnormal wing postures, (2) abnormal calling song, and (3) loss of the circadian locomotor rhythm, while for Gb'DopRI, defects of wing posture and morphology were found. These results indicate that the cricket has the potential to become a novel model system to explore human neuronal pathogenic mechanisms and to screen therapeutic drugs by RNAi.

Original languageEnglish
Pages (from-to)2025-2033
Number of pages9
JournalDevelopmental Dynamics
Volume238
Issue number8
DOIs
Publication statusPublished - Aug 2008

Fingerprint

Gryllidae
Dopamine Receptors
RNA Interference
Intellectual Disability
Posture
Genes
Music
Circadian Rhythm
Complementary DNA
Phenotype
Pharmaceutical Preparations
Therapeutics

Keywords

  • Calling song
  • Circadian locomotor rhythm
  • Cricket
  • Dopamine receptor
  • Fragile X mental retardation
  • RNA interference
  • Wing posture

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Loss-of-function analyses of the fragile X-related and dopamine receptor genes by RNA interference in the cricket Gryllus bimaculatus. / Hamada, Aska; Miyawaki, Katsuyuki; Honda-sumi, Eri; Tomioka, Kenji; Mito, Taro; Ohuchi, Hideyo; Noji, Sumihare.

In: Developmental Dynamics, Vol. 238, No. 8, 08.2008, p. 2025-2033.

Research output: Contribution to journalArticle

Hamada, Aska ; Miyawaki, Katsuyuki ; Honda-sumi, Eri ; Tomioka, Kenji ; Mito, Taro ; Ohuchi, Hideyo ; Noji, Sumihare. / Loss-of-function analyses of the fragile X-related and dopamine receptor genes by RNA interference in the cricket Gryllus bimaculatus. In: Developmental Dynamics. 2008 ; Vol. 238, No. 8. pp. 2025-2033.
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