Loss of CG Methylation in Marchantia polymorpha Causes Disorganization of Cell Division and Reveals Unique DNA Methylation Regulatory Mechanisms of Non-CG Methylation

Yoko Ikeda, Ryuichi Nishihama, Shohei Yamaoka, Mario A. Arteaga-Vazquez, Adolfo Aguilar-Cruz, Daniel Grimanelli, Romain Pogorelcnik, Robert A. Martienssen, Katsuyuki T. Yamato, Takayuki Kohchi, Takashi Hirayama, Olivier Mathieu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

DNA methylation is an epigenetic mark that ensures silencing of transposable elements (TEs) and affects gene expression in many organisms. The function of different DNA methylation regulatory pathways has been largely characterized in the model plant Arabidopsis thaliana. However, far less is known about DNA methylation regulation and functions in basal land plants. Here we focus on the liverwort Marchantia polymorpha, an emerging model species that represents a basal lineage of land plants. We identified MpMET, the M. polymorpha ortholog of the METHYLTRANSFERASE 1 (MET1) gene required for maintenance of methylation at CG sites in angiosperms. We generated Mpmet mutants using the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein9) system, which showed a significant loss of CG methylation and severe morphological changes and developmental defects. The mutants developed many adventitious shoot-like structures, suggesting that MpMET is required for maintaining differentiated cellular identities in the gametophyte. Even though numerous TEs were up-regulated, non-CG methylation was generally highly increased at TEs in the Mpmet mutants. Closer inspection of CHG methylation revealed features unique to M. polymorpha. Methylation of CCG sites in M. polymorpha does not depend on MET1, unlike in A. thaliana and Physcomitrella patens. Our results highlight the diversity of non-CG methylation regulatory mechanisms in plants.

Original languageEnglish
Pages (from-to)2421-2431
Number of pages11
JournalPlant & cell physiology
Volume59
Issue number12
DOIs
Publication statusPublished - Dec 1 2018

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Marchantia
Marchantia polymorpha
DNA methylation
DNA Methylation
Cell Division
methylation
Methylation
cell division
DNA Transposable Elements
Clustered Regularly Interspaced Short Palindromic Repeats
transposons
Embryophyta
embryophytes
Arabidopsis
mutants
Arabidopsis thaliana
Bryopsida
Transcriptional Silencer Elements
Hepatophyta
Angiosperms

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

Cite this

Loss of CG Methylation in Marchantia polymorpha Causes Disorganization of Cell Division and Reveals Unique DNA Methylation Regulatory Mechanisms of Non-CG Methylation. / Ikeda, Yoko; Nishihama, Ryuichi; Yamaoka, Shohei; Arteaga-Vazquez, Mario A.; Aguilar-Cruz, Adolfo; Grimanelli, Daniel; Pogorelcnik, Romain; Martienssen, Robert A.; Yamato, Katsuyuki T.; Kohchi, Takayuki; Hirayama, Takashi; Mathieu, Olivier.

In: Plant & cell physiology, Vol. 59, No. 12, 01.12.2018, p. 2421-2431.

Research output: Contribution to journalArticle

Ikeda, Y, Nishihama, R, Yamaoka, S, Arteaga-Vazquez, MA, Aguilar-Cruz, A, Grimanelli, D, Pogorelcnik, R, Martienssen, RA, Yamato, KT, Kohchi, T, Hirayama, T & Mathieu, O 2018, 'Loss of CG Methylation in Marchantia polymorpha Causes Disorganization of Cell Division and Reveals Unique DNA Methylation Regulatory Mechanisms of Non-CG Methylation', Plant & cell physiology, vol. 59, no. 12, pp. 2421-2431. https://doi.org/10.1093/pcp/pcy161
Ikeda, Yoko ; Nishihama, Ryuichi ; Yamaoka, Shohei ; Arteaga-Vazquez, Mario A. ; Aguilar-Cruz, Adolfo ; Grimanelli, Daniel ; Pogorelcnik, Romain ; Martienssen, Robert A. ; Yamato, Katsuyuki T. ; Kohchi, Takayuki ; Hirayama, Takashi ; Mathieu, Olivier. / Loss of CG Methylation in Marchantia polymorpha Causes Disorganization of Cell Division and Reveals Unique DNA Methylation Regulatory Mechanisms of Non-CG Methylation. In: Plant & cell physiology. 2018 ; Vol. 59, No. 12. pp. 2421-2431.
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