Loss of bag-1 immunoreactivity in rat brain after transient middle cerebral artery occlusion

Takeshi Hayashi, Ken Ichi Sakai, Chihoko Sasaki, Yasuto Itoyama, Koji Abe

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Although bag-1 is a strong apoptosis repressor protein, its functions in normal or injured brains are not fully understood. In the present study, we investigated expression of bag-1 protein in rat brain after transient middle cerebral artery (MCA) occlusion, and compared the results with that of terminal deoxynucleotidyl transferase-mediated dUTP-biotin end labeling (TUNEL). Immunohistochemical analysis revealed that neuronal, choroid plexus, and ependymal cells were positively stained in the sham control brain. After 90 min of transient MCA occlusion, immunoreactivity for bag-1 progressively decreased from 3 to 48 h in the nuclei of neurons. Western blot analysis revealed that immunoreactive bag-1 was markedly decreased in the nuclear fraction. In contrast, cytosolic and mitochondrial fractions showed no or only slight change after the ischemia. TUNEL positive cells appeared at 48 h after the reperfusion, which was preceded by loss of bag-1 immunoreactivity. The present results suggest that bag-1 plays some roles in normal neuronal function, and its loss may be involved in neuronal cell death after ischemia. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)496-500
Number of pages5
JournalBrain Research
Volume852
Issue number2
DOIs
Publication statusPublished - Jan 10 2000

Fingerprint

Middle Cerebral Artery Infarction
Biotin
Brain
Ischemia
Repressor Proteins
Choroid Plexus
DNA Nucleotidylexotransferase
Transferases
Reperfusion
Cell Death
Western Blotting
Apoptosis
Neurons

Keywords

  • Apoptosis
  • Bag-1
  • Brain
  • Ischemia
  • Rat

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Loss of bag-1 immunoreactivity in rat brain after transient middle cerebral artery occlusion. / Hayashi, Takeshi; Sakai, Ken Ichi; Sasaki, Chihoko; Itoyama, Yasuto; Abe, Koji.

In: Brain Research, Vol. 852, No. 2, 10.01.2000, p. 496-500.

Research output: Contribution to journalArticle

Hayashi, Takeshi ; Sakai, Ken Ichi ; Sasaki, Chihoko ; Itoyama, Yasuto ; Abe, Koji. / Loss of bag-1 immunoreactivity in rat brain after transient middle cerebral artery occlusion. In: Brain Research. 2000 ; Vol. 852, No. 2. pp. 496-500.
@article{b277b5f822404303aa7380cc83664dd6,
title = "Loss of bag-1 immunoreactivity in rat brain after transient middle cerebral artery occlusion",
abstract = "Although bag-1 is a strong apoptosis repressor protein, its functions in normal or injured brains are not fully understood. In the present study, we investigated expression of bag-1 protein in rat brain after transient middle cerebral artery (MCA) occlusion, and compared the results with that of terminal deoxynucleotidyl transferase-mediated dUTP-biotin end labeling (TUNEL). Immunohistochemical analysis revealed that neuronal, choroid plexus, and ependymal cells were positively stained in the sham control brain. After 90 min of transient MCA occlusion, immunoreactivity for bag-1 progressively decreased from 3 to 48 h in the nuclei of neurons. Western blot analysis revealed that immunoreactive bag-1 was markedly decreased in the nuclear fraction. In contrast, cytosolic and mitochondrial fractions showed no or only slight change after the ischemia. TUNEL positive cells appeared at 48 h after the reperfusion, which was preceded by loss of bag-1 immunoreactivity. The present results suggest that bag-1 plays some roles in normal neuronal function, and its loss may be involved in neuronal cell death after ischemia. Copyright (C) 2000 Elsevier Science B.V.",
keywords = "Apoptosis, Bag-1, Brain, Ischemia, Rat",
author = "Takeshi Hayashi and Sakai, {Ken Ichi} and Chihoko Sasaki and Yasuto Itoyama and Koji Abe",
year = "2000",
month = "1",
day = "10",
doi = "10.1016/S0006-8993(99)02266-0",
language = "English",
volume = "852",
pages = "496--500",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Loss of bag-1 immunoreactivity in rat brain after transient middle cerebral artery occlusion

AU - Hayashi, Takeshi

AU - Sakai, Ken Ichi

AU - Sasaki, Chihoko

AU - Itoyama, Yasuto

AU - Abe, Koji

PY - 2000/1/10

Y1 - 2000/1/10

N2 - Although bag-1 is a strong apoptosis repressor protein, its functions in normal or injured brains are not fully understood. In the present study, we investigated expression of bag-1 protein in rat brain after transient middle cerebral artery (MCA) occlusion, and compared the results with that of terminal deoxynucleotidyl transferase-mediated dUTP-biotin end labeling (TUNEL). Immunohistochemical analysis revealed that neuronal, choroid plexus, and ependymal cells were positively stained in the sham control brain. After 90 min of transient MCA occlusion, immunoreactivity for bag-1 progressively decreased from 3 to 48 h in the nuclei of neurons. Western blot analysis revealed that immunoreactive bag-1 was markedly decreased in the nuclear fraction. In contrast, cytosolic and mitochondrial fractions showed no or only slight change after the ischemia. TUNEL positive cells appeared at 48 h after the reperfusion, which was preceded by loss of bag-1 immunoreactivity. The present results suggest that bag-1 plays some roles in normal neuronal function, and its loss may be involved in neuronal cell death after ischemia. Copyright (C) 2000 Elsevier Science B.V.

AB - Although bag-1 is a strong apoptosis repressor protein, its functions in normal or injured brains are not fully understood. In the present study, we investigated expression of bag-1 protein in rat brain after transient middle cerebral artery (MCA) occlusion, and compared the results with that of terminal deoxynucleotidyl transferase-mediated dUTP-biotin end labeling (TUNEL). Immunohistochemical analysis revealed that neuronal, choroid plexus, and ependymal cells were positively stained in the sham control brain. After 90 min of transient MCA occlusion, immunoreactivity for bag-1 progressively decreased from 3 to 48 h in the nuclei of neurons. Western blot analysis revealed that immunoreactive bag-1 was markedly decreased in the nuclear fraction. In contrast, cytosolic and mitochondrial fractions showed no or only slight change after the ischemia. TUNEL positive cells appeared at 48 h after the reperfusion, which was preceded by loss of bag-1 immunoreactivity. The present results suggest that bag-1 plays some roles in normal neuronal function, and its loss may be involved in neuronal cell death after ischemia. Copyright (C) 2000 Elsevier Science B.V.

KW - Apoptosis

KW - Bag-1

KW - Brain

KW - Ischemia

KW - Rat

UR - http://www.scopus.com/inward/record.url?scp=0033986390&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033986390&partnerID=8YFLogxK

U2 - 10.1016/S0006-8993(99)02266-0

DO - 10.1016/S0006-8993(99)02266-0

M3 - Article

C2 - 10678782

AN - SCOPUS:0033986390

VL - 852

SP - 496

EP - 500

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 2

ER -