TY - JOUR
T1 - Loss of bag-1 immunoreactivity in rat brain after transient middle cerebral artery occlusion
AU - Hayashi, Takeshi
AU - Sakai, Ken Ichi
AU - Sasaki, Chihoko
AU - Itoyama, Yasuto
AU - Abe, Koji
N1 - Funding Information:
This work was partly supported by Grant-in-Aid for Scientific Research (A) 10176204 and (B) 09470151 from the Ministry of Education, Science and Culture of Japan, by a Grant (K. Tashiro) from the Ministry of Health and Welfare of Japan, by Suzuken Memorial Sciences Foundation, and by Japan Heart Foundation Dr. Hiroshi Irisawa Commemorative Research Grant.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2000/1/10
Y1 - 2000/1/10
N2 - Although bag-1 is a strong apoptosis repressor protein, its functions in normal or injured brains are not fully understood. In the present study, we investigated expression of bag-1 protein in rat brain after transient middle cerebral artery (MCA) occlusion, and compared the results with that of terminal deoxynucleotidyl transferase-mediated dUTP-biotin end labeling (TUNEL). Immunohistochemical analysis revealed that neuronal, choroid plexus, and ependymal cells were positively stained in the sham control brain. After 90 min of transient MCA occlusion, immunoreactivity for bag-1 progressively decreased from 3 to 48 h in the nuclei of neurons. Western blot analysis revealed that immunoreactive bag-1 was markedly decreased in the nuclear fraction. In contrast, cytosolic and mitochondrial fractions showed no or only slight change after the ischemia. TUNEL positive cells appeared at 48 h after the reperfusion, which was preceded by loss of bag-1 immunoreactivity. The present results suggest that bag-1 plays some roles in normal neuronal function, and its loss may be involved in neuronal cell death after ischemia. Copyright (C) 2000 Elsevier Science B.V.
AB - Although bag-1 is a strong apoptosis repressor protein, its functions in normal or injured brains are not fully understood. In the present study, we investigated expression of bag-1 protein in rat brain after transient middle cerebral artery (MCA) occlusion, and compared the results with that of terminal deoxynucleotidyl transferase-mediated dUTP-biotin end labeling (TUNEL). Immunohistochemical analysis revealed that neuronal, choroid plexus, and ependymal cells were positively stained in the sham control brain. After 90 min of transient MCA occlusion, immunoreactivity for bag-1 progressively decreased from 3 to 48 h in the nuclei of neurons. Western blot analysis revealed that immunoreactive bag-1 was markedly decreased in the nuclear fraction. In contrast, cytosolic and mitochondrial fractions showed no or only slight change after the ischemia. TUNEL positive cells appeared at 48 h after the reperfusion, which was preceded by loss of bag-1 immunoreactivity. The present results suggest that bag-1 plays some roles in normal neuronal function, and its loss may be involved in neuronal cell death after ischemia. Copyright (C) 2000 Elsevier Science B.V.
KW - Apoptosis
KW - Bag-1
KW - Brain
KW - Ischemia
KW - Rat
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U2 - 10.1016/S0006-8993(99)02266-0
DO - 10.1016/S0006-8993(99)02266-0
M3 - Article
C2 - 10678782
AN - SCOPUS:0033986390
VL - 852
SP - 496
EP - 500
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 2
ER -