Long-term isolation of the coastal plant Calystegia soldanella (Convolvulaceae) in ancient freshwater Lake Biwa, Japan

Asuka Noda, Yuki Mitsui, Hajime Ikeda, Hiroaki Setoguchi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Lake Biwa is an ancient freshwater lake that was formed approximately 4 Mya and harbours many coastal plants that commonly inhabit the seashore. We used chloroplast DNA haplotype analysis using two spacer sequences and simple sequence repeat (SSR) analysis using eight nuclear microsatellite markers to detect genomic signatures indicating long-term isolation of inland populations of Calystegia soldanella in Lake Biwa from coastal populations. We used 348 samples from 63 populations for haplotype analysis and 478 samples from 27 populations for SSR analysis covering the inland and coastal distribution of the species. We detected seven haplotypes, and the distribution pattern of these haplotypes was geographically highly structured between Lake Biwa and the coast. Nuclear SSR analysis also supported genetic differentiation between Lake Biwa and coastal populations (analyses of molecular variance, 43%), and the grouping of Lake Biwa and coastal populations by a Neighbour-joining tree. In addition, genetic diversity of the inland populations (mean HE = 0.153) was significantly lower than that of coastal populations (mean HE = 0.328). These results suggested that inland populations at Lake Biwa have been isolated from coastal populations for a very long time. The inland populations most likely experienced a bottleneck effect, resulting in sufficient in situ genetic divergence to clearly distinguish them from coastal populations.

Original languageEnglish
Pages (from-to)51-66
Number of pages16
JournalBiological Journal of the Linnean Society
Volume102
Issue number1
DOIs
Publication statusPublished - Jan 2011
Externally publishedYes

Fingerprint

Convolvulaceae
Japan
lakes
lake
haplotypes
microsatellite repeats
genetic variation
Calystegia soldanella
chloroplast
genetic differentiation
genomics
harbor
divergence
analysis
harbors (waterways)
DNA
chloroplast DNA
coast
biogeography

Keywords

  • Haplotype
  • Isolation
  • Landlocked
  • Microsatellite
  • Phylogeography

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

Cite this

Long-term isolation of the coastal plant Calystegia soldanella (Convolvulaceae) in ancient freshwater Lake Biwa, Japan. / Noda, Asuka; Mitsui, Yuki; Ikeda, Hajime; Setoguchi, Hiroaki.

In: Biological Journal of the Linnean Society, Vol. 102, No. 1, 01.2011, p. 51-66.

Research output: Contribution to journalArticle

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