Long-term inhibition of angiotensin prevents reduction of periarterial innervation of calcitonin gene-related peptide (CGRP)-containing nerves in spontaneously hypertensive rats

Narumi Hobara, Noriko Gessei-Tsutsumi, Mitsuhiro Goda, Fusako Takayama, Shinji Akiyama, Yuji Kurosaki, Hiromu Kawasaki

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The aim of this study was to investigate age-related changes in the density of calcitonin gene-related peptide (CGRP)-containing nerve fibers in spontaneously hypertensive rats (SHR) and the effects of long-term inhibition of the renin-angiotensin system on these changes. The density of immunocytochemically stained nerve fibers in the mesenteric artery was quantified by computer-assisted image processing. An age-related decrease in the density of CGRP-like immunoreactive (LI)-containing nerve fivers but not neuropeptide Y (NPY)-LI-containing sympathetic nerve fibers was found in the mesenteric artery of SHR but not Wistar Kyoto rats (WKY). The density of NPY-LI-containing sympathetic nerve fibers was significantly greater in SHR than in WKY. SHR were treated for 7 weeks with angiotensin converting enzyme inhibitor (0.005% temocapril), angiotensin II type-1 (AT,) receptor antagonist (0.025% losartan) or vasodilator (0.01% hydralazine) in their drinking water. Each drug treatment significantly lowered the systolic blood pressure measured by tail-cuff method. Long-term treatment of SHR with temocapril and losartan significantly increased the density of CGRP-LI-containing nerve fibers in mesenteric arteries. However, the density after hydralazine treatment was similar to the level in non-treated SHR. The density of NPY-LI-containing nerve fibers was not increased by any of the drug treatments. These results suggest that long-term inhibition of the renin-angiotensin system in SHR prevents remodeling of CGRPergic nerve fibers and prevents the reduction of CGRPergic nerve function.

Original languageEnglish
Pages (from-to)465-474
Number of pages10
JournalHypertension Research
Volume28
Issue number5
DOIs
Publication statusPublished - May 2005

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Calcitonin Gene-Related Peptide
Angiotensins
Inbred SHR Rats
Nerve Fibers
Mesenteric Arteries
Neuropeptide Y
Adrenergic Fibers
Hydralazine
Losartan
Inbred WKY Rats
Renin-Angiotensin System
Blood Pressure
Angiotensin II Type 1 Receptor Blockers
Computer-Assisted Image Processing
Vasodilator Agents
Angiotensin-Converting Enzyme Inhibitors
Drinking Water
Pharmaceutical Preparations
Tail

Keywords

  • Angiotensin converting enzyme inhibitor
  • Angiotensin II type-1 receptor antagonist
  • Calcitonin gene-related peptide (CGRP)-containing vasodilator nerve innervation
  • Neuropeptide Y (NPY)-containing sympathetic nerve innervation
  • Spontaneously hypertensive rat

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Long-term inhibition of angiotensin prevents reduction of periarterial innervation of calcitonin gene-related peptide (CGRP)-containing nerves in spontaneously hypertensive rats. / Hobara, Narumi; Gessei-Tsutsumi, Noriko; Goda, Mitsuhiro; Takayama, Fusako; Akiyama, Shinji; Kurosaki, Yuji; Kawasaki, Hiromu.

In: Hypertension Research, Vol. 28, No. 5, 05.2005, p. 465-474.

Research output: Contribution to journalArticle

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