Long-term effect of systemic RNA interference on circadian clock genes in hemimetabolous insects

Outa Uryu, Yuichi Kamae, Kenji Tomioka, Taishi Yoshii

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

RNA interference (RNAi) strategy, which enables gene-specific knock-down of transcripts, has been spread across a wide area of insect studies for investigating gene function without regard to model and non-model insects. This technique is of particular benefit to promote molecular studies on non-model insects. However, the optimal conditions for RNAi are still not well understood because of its variable efficiency depending on the species, target genes, and experimental conditions. To apply RNAi technique to long-running experiments such as chronobiological studies, the effects of RNAi have to persist throughout the experiment. In this study, we attempted to determine the optimal concentration of double-stranded RNA (dsRNA) for systemic RNAi and its effective period in two different insect species, the cricket Gryllus bimaculatus and the firebrat Thermobia domestica. In both species, higher concentrations of dsRNA principally yielded a more efficient knock-down of mRNA levels of tested clock genes, although the effect depended on the gene and the species. Surprisingly, the effect of the RNAi reached its maximum effect 1-2. weeks and 1. month after the injection of dsRNA in the crickets and the firebrats, respectively, suggesting a slow but long-term effect of RNAi. Our study provides fundamental information for utilizing RNAi technique in any long-running experiment.

Original languageEnglish
Pages (from-to)494-499
Number of pages6
JournalJournal of Insect Physiology
Volume59
Issue number4
DOIs
Publication statusPublished - Apr 1 2013

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Keywords

  • Clock gene
  • Cricket
  • Double-stranded RNA
  • Firebrat
  • Gene knock-down
  • RNA interference

ASJC Scopus subject areas

  • Physiology
  • Insect Science

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