Long-chain aldehyde dehydrogenase that participates in n-alkane utilization and wax ester synthesis in Acinetobacter sp. strain M-1

T. Ishige, Akiwo Tani, Y. Sakai, N. Kato

Research output: Contribution to journalArticle

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Abstract

A long-chain aldehyde dehydrogenase, Ald1, was found in a soluble fraction of Acinetobacter sp. strain M-1 cells grown on n-hexadecane as a sole carbon source. The gene (ald1) was cloned from the chromosomal DNA of the bacterium. The open reading frame of ald1 was 1,512 bp long, corresponding to a protein of 503 amino acid residues (molecular mass, 55,496 Da), and the deduced amino acid sequence showed high similarity to those of various aldehyde dehydrogenases. The ald1 gene was stably expressed in Escherichia coli, and the gene product (recombinant Ald1 [rAld1]) was purified to apparent homogeneity by gel electrophoresis. rAld1 showed enzyme activity toward n-alkanals (C4 to C14), with a preference for longer carbon chains within the tested range; the highest activity was obtained with tetradecanal. The ald1 gene was disrupted by homologous recombination on the Acinetobacter genome. Although the ald1 disruptant (ald1Δ) strain still had the ability to grow on n-hexadecane to some extent, its aldehyde dehydrogenase activity toward n-tetradecanal was reduced to half the level of the wild-type strain. Under nitrogen-limiting conditions, the accumulation of intracellular wax esters in the ald1Δ strain became much lower than that in the wild-type strain. These and other results imply that a soluble long-chain aldehyde dehydrogenase indeed plays important roles both in growth on n-alkane and in wax ester formation in Acinetobacter sp. strain M-1.

Original languageEnglish
Pages (from-to)3481-3486
Number of pages6
JournalApplied and Environmental Microbiology
Volume66
Issue number8
DOIs
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

long-chain-aldehyde dehydrogenase
aldehyde dehydrogenase
wax esters
Acinetobacter
Alkanes
Waxes
aldehyde
wax
alkane
alkanes
ester
Esters
Aldehyde Dehydrogenase
synthesis
gene
Genes
hexadecane
Carbon
amino acid
Homologous Recombination

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

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Long-chain aldehyde dehydrogenase that participates in n-alkane utilization and wax ester synthesis in Acinetobacter sp. strain M-1. / Ishige, T.; Tani, Akiwo; Sakai, Y.; Kato, N.

In: Applied and Environmental Microbiology, Vol. 66, No. 8, 2000, p. 3481-3486.

Research output: Contribution to journalArticle

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