Locus- and domain-dependent control of DNA methylation at mouse B1 retrotransposons during male germ cell development

Kenji Ichiyanagi, Yungfeng Li, Toshiaki Watanabe, Tomoko Ichiyanagi, Kei Fukuda, Junko Kitayama, Yasuhiro Yamamoto, Satomi Kuramochi-Miyagawa, Toru Nakano, Yukihiro Yabuta, Yoshiyuki Seki, Mitinori Saitou, Hiroyuki Sasaki

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

In mammals, germ cells undergo striking dynamic changes in DNA methylation during their development. However, the dynamics and mode of methylation are poorly understood for short interspersed elements (SINEs) dispersed throughout the genome. We investigated the DNA methylation status of mouse B1 SINEs in male germ cells at different developmental stages. B1 elements showed a large locus-to-locus variation in methylation; loci close to RNA polymerase II promoters were hypomethylated, while most others were hypermethylated. Interestingly, a mutation that eliminates Piwi-interacting RNAs (piRNAs), which are involved in methylation of long interspersed elements (LINEs), did not affect the level of B1 methylation, implying a piRNA-independent mechanism. Methylation at B1 loci in SINE-poor genomic domains showed a higher dependency on the de novo DNA methyltransferase DNMT3A but not on DNMT3B, suggesting that DNMT3A plays a major role in methylation of these domains. We also found that many genes specifically expressed in the testis possess B1 elements in their promoters, suggesting the involvement of B1 methylation in transcriptional regulation. Taken altogether, our results not only reveal the dynamics and mode of SINE methylation but also suggest how the DNA methylation profile is created in the germline by a pair of DNA methyltransferases.

Original languageEnglish
Pages (from-to)2058-2066
Number of pages9
JournalGenome Research
Volume21
Issue number12
DOIs
Publication statusPublished - Dec 2011
Externally publishedYes

Fingerprint

Retroelements
DNA Methylation
Germ Cells
Methylation
Methyltransferases
Small Interfering RNA
RNA Polymerase II
DNA
Testis
Mammals
Genome
Mutation

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Ichiyanagi, K., Li, Y., Watanabe, T., Ichiyanagi, T., Fukuda, K., Kitayama, J., ... Sasaki, H. (2011). Locus- and domain-dependent control of DNA methylation at mouse B1 retrotransposons during male germ cell development. Genome Research, 21(12), 2058-2066. https://doi.org/10.1101/gr.123679.111

Locus- and domain-dependent control of DNA methylation at mouse B1 retrotransposons during male germ cell development. / Ichiyanagi, Kenji; Li, Yungfeng; Watanabe, Toshiaki; Ichiyanagi, Tomoko; Fukuda, Kei; Kitayama, Junko; Yamamoto, Yasuhiro; Kuramochi-Miyagawa, Satomi; Nakano, Toru; Yabuta, Yukihiro; Seki, Yoshiyuki; Saitou, Mitinori; Sasaki, Hiroyuki.

In: Genome Research, Vol. 21, No. 12, 12.2011, p. 2058-2066.

Research output: Contribution to journalArticle

Ichiyanagi, K, Li, Y, Watanabe, T, Ichiyanagi, T, Fukuda, K, Kitayama, J, Yamamoto, Y, Kuramochi-Miyagawa, S, Nakano, T, Yabuta, Y, Seki, Y, Saitou, M & Sasaki, H 2011, 'Locus- and domain-dependent control of DNA methylation at mouse B1 retrotransposons during male germ cell development', Genome Research, vol. 21, no. 12, pp. 2058-2066. https://doi.org/10.1101/gr.123679.111
Ichiyanagi, Kenji ; Li, Yungfeng ; Watanabe, Toshiaki ; Ichiyanagi, Tomoko ; Fukuda, Kei ; Kitayama, Junko ; Yamamoto, Yasuhiro ; Kuramochi-Miyagawa, Satomi ; Nakano, Toru ; Yabuta, Yukihiro ; Seki, Yoshiyuki ; Saitou, Mitinori ; Sasaki, Hiroyuki. / Locus- and domain-dependent control of DNA methylation at mouse B1 retrotransposons during male germ cell development. In: Genome Research. 2011 ; Vol. 21, No. 12. pp. 2058-2066.
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