Location of PsbY in oxygen-evolving photosystem II revealed by mutagenesis and X-ray crystallography

Keisuke Kawakami; Masako Iwai; Masahiko Ikeuchi; Nobuo Kamiya; Jian Ren Shen

doi:10.1016/j.febslet.2007.09.036

Location of PsbY in oxygen-evolving photosystem II revealed by mutagenesis and X-ray crystallography

Keisuke Kawakami, Masako Iwai, Masahiko Ikeuchi, Nobuo Kamiya, Jian Ren Shen

Research Institute for Interdisciplinary Science

Research output: Contribution to journal › Article › peer-review

53 Citations (Scopus)

Abstract

PsbY is one of the low molecular mass subunits of oxygen-evolving photosystem II (PSII). Its location, however, has not been identified in the current crystal structure of PSII. We constructed a PsbY-deletion mutant of Thermosynechococcus elongatus, crystallized, and analyzed the crystal structure of the mutant PSII dimer. The results obtained showed that PsbY is located in the periphery of PSII close to the α- and β-subunits of cytochrome b559, which corresponded to an unassigned helix in the 3.7 Å structure of T. vulcanus or helix X2 in the 3.0 Å structure of T. elongatus. Our results also indicated that the C-terminal loop of PsbY is protruded toward the stromal side, instead of the lumenal side predicted previously.

Original language	English
Pages (from-to)	4983-4987
Number of pages	5
Journal	FEBS Letters
Volume	581
Issue number	25
DOIs	https://doi.org/10.1016/j.febslet.2007.09.036
Publication status	Published - Oct 16 2007

Keywords

Crystal structure
Crystallization
Membrane proteins
Mutant
Photosystem II
PsbY

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2007.09.036

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title = "Location of PsbY in oxygen-evolving photosystem II revealed by mutagenesis and X-ray crystallography",

abstract = "PsbY is one of the low molecular mass subunits of oxygen-evolving photosystem II (PSII). Its location, however, has not been identified in the current crystal structure of PSII. We constructed a PsbY-deletion mutant of Thermosynechococcus elongatus, crystallized, and analyzed the crystal structure of the mutant PSII dimer. The results obtained showed that PsbY is located in the periphery of PSII close to the α- and β-subunits of cytochrome b559, which corresponded to an unassigned helix in the 3.7 {\AA} structure of T. vulcanus or helix X2 in the 3.0 {\AA} structure of T. elongatus. Our results also indicated that the C-terminal loop of PsbY is protruded toward the stromal side, instead of the lumenal side predicted previously.",

keywords = "Crystal structure, Crystallization, Membrane proteins, Mutant, Photosystem II, PsbY",

author = "Keisuke Kawakami and Masako Iwai and Masahiko Ikeuchi and Nobuo Kamiya and Shen, {Jian Ren}",

note = "Funding Information: This work was supported by a Grant-in-Aid for Young Scientists (B) (17770042) (to M. Iwai), a Grant-in-Aid for Scientific Research on Priority Areas (Structures of Biological Macromolecular Assemblies), and a Grant-in-Aid for Creative Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan.",

year = "2007",

month = oct,

day = "16",

doi = "10.1016/j.febslet.2007.09.036",

language = "English",

volume = "581",

pages = "4983--4987",

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T1 - Location of PsbY in oxygen-evolving photosystem II revealed by mutagenesis and X-ray crystallography

AU - Kawakami, Keisuke

AU - Iwai, Masako

AU - Ikeuchi, Masahiko

AU - Kamiya, Nobuo

AU - Shen, Jian Ren

N1 - Funding Information: This work was supported by a Grant-in-Aid for Young Scientists (B) (17770042) (to M. Iwai), a Grant-in-Aid for Scientific Research on Priority Areas (Structures of Biological Macromolecular Assemblies), and a Grant-in-Aid for Creative Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

PY - 2007/10/16

Y1 - 2007/10/16

N2 - PsbY is one of the low molecular mass subunits of oxygen-evolving photosystem II (PSII). Its location, however, has not been identified in the current crystal structure of PSII. We constructed a PsbY-deletion mutant of Thermosynechococcus elongatus, crystallized, and analyzed the crystal structure of the mutant PSII dimer. The results obtained showed that PsbY is located in the periphery of PSII close to the α- and β-subunits of cytochrome b559, which corresponded to an unassigned helix in the 3.7 Å structure of T. vulcanus or helix X2 in the 3.0 Å structure of T. elongatus. Our results also indicated that the C-terminal loop of PsbY is protruded toward the stromal side, instead of the lumenal side predicted previously.

AB - PsbY is one of the low molecular mass subunits of oxygen-evolving photosystem II (PSII). Its location, however, has not been identified in the current crystal structure of PSII. We constructed a PsbY-deletion mutant of Thermosynechococcus elongatus, crystallized, and analyzed the crystal structure of the mutant PSII dimer. The results obtained showed that PsbY is located in the periphery of PSII close to the α- and β-subunits of cytochrome b559, which corresponded to an unassigned helix in the 3.7 Å structure of T. vulcanus or helix X2 in the 3.0 Å structure of T. elongatus. Our results also indicated that the C-terminal loop of PsbY is protruded toward the stromal side, instead of the lumenal side predicted previously.

KW - Crystal structure

KW - Crystallization

KW - Membrane proteins

KW - Mutant

KW - Photosystem II

KW - PsbY

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Location of PsbY in oxygen-evolving photosystem II revealed by mutagenesis and X-ray crystallography

Abstract

Keywords

ASJC Scopus subject areas

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