Location of PsbY in oxygen-evolving photosystem II revealed by mutagenesis and X-ray crystallography

Keisuke Kawakami, Masako Iwai, Masahiko Ikeuchi, Nobuo Kamiya, Jian Ren Shen

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

PsbY is one of the low molecular mass subunits of oxygen-evolving photosystem II (PSII). Its location, however, has not been identified in the current crystal structure of PSII. We constructed a PsbY-deletion mutant of Thermosynechococcus elongatus, crystallized, and analyzed the crystal structure of the mutant PSII dimer. The results obtained showed that PsbY is located in the periphery of PSII close to the α- and β-subunits of cytochrome b559, which corresponded to an unassigned helix in the 3.7 Å structure of T. vulcanus or helix X2 in the 3.0 Å structure of T. elongatus. Our results also indicated that the C-terminal loop of PsbY is protruded toward the stromal side, instead of the lumenal side predicted previously.

Original languageEnglish
Pages (from-to)4983-4987
Number of pages5
JournalFEBS Letters
Volume581
Issue number25
DOIs
Publication statusPublished - Oct 16 2007
Externally publishedYes

Keywords

  • Crystal structure
  • Crystallization
  • Membrane proteins
  • Mutant
  • Photosystem II
  • PsbY

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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