Location of proliferating gingival cells following toothbrushing stimulation

T. Tomofuji, T. Sakamoto, Daisuke Ekuni, T. Yamamoto, T. Watanabe

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objectives: Mechanical stimulation by toothbrushing promotes healing of gingivitis through accelerating cell proliferation. Junctional epithelium proliferates at periodontal pocket formation. A question is arisen whether toothbrushing contributes to the repair of gingival inflammation or deterioration of pocket formation. The location of proliferating cells in gingiva stimulated mechanically by toothbrushing was investigated. Materials and methods: A total of 24 teeth of dogs underwent daily plaque removal with a curette (plaque removal) or both plaque removal and toothbrushing (toothbrushing). Proliferative activity of gingival cells in six individual zones was evaluated by assaying expression of proliferating cell nuclear antigen (PCNA). Results: Toothbrushing increased densities of PCNA-positive basal cells in the junctional epithelium, connective tissues adjacent to the junctional epithelium, the alveolar bone of the oral epithelial side and the oral epithelium. However, the densities of PCNA-positive cells at the apical portion of the junctional epithelium, connective tissues adjacent to the cementum and the alveolar bone of the periodontal ligament side did not increase following toothbrushing. Conclusions: Toothbrushing promotes proliferation of gingival cells other than fibroblasts in periodontium and basal cells in the apical portion of the junctional epithelium. The repair of periodontal tissues might be promoted by toothbrushing within the limit of the direct mechanical stimulation.

Original languageEnglish
Pages (from-to)77-81
Number of pages5
JournalOral Diseases
Volume13
Issue number1
DOIs
Publication statusPublished - Jan 2007

Fingerprint

Toothbrushing
Epithelial Attachment
Proliferating Cell Nuclear Antigen
Connective Tissue
Cell Proliferation
Periodontal Pocket
Dental Cementum
Bone and Bones
Periodontium
Periodontal Ligament
Gingivitis
Gingiva
Tooth
Epithelium
Fibroblasts
Dogs
Inflammation

Keywords

  • Animal studies
  • Gingival cells
  • Mechanical stimulation
  • Proliferating cell nuclear antigen
  • Toothbrushing

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Location of proliferating gingival cells following toothbrushing stimulation. / Tomofuji, T.; Sakamoto, T.; Ekuni, Daisuke; Yamamoto, T.; Watanabe, T.

In: Oral Diseases, Vol. 13, No. 1, 01.2007, p. 77-81.

Research output: Contribution to journalArticle

Tomofuji, T. ; Sakamoto, T. ; Ekuni, Daisuke ; Yamamoto, T. ; Watanabe, T. / Location of proliferating gingival cells following toothbrushing stimulation. In: Oral Diseases. 2007 ; Vol. 13, No. 1. pp. 77-81.
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