Location of micropyles and early embryonic development of the two-spotted cricket Gryllus bimaculatus (Insecta, Orthoptera)

Isao Sarashina, Taro Mito, Michiko Saito, Hiroyuki Uneme, Katsuyuki Miyawaki, Yohei Shinmyo, Hideyo Ohuchi, Sumihare Noji

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Early embryogenesis of the two-spotted cricket Gryllus bimaculatus was examined by scanning electron microscopy and several fluorescence staining methods, with special reference to these four issues: (i) the location of micropyles; (ii) the transfer of the female pronucleus following meiosis; (iii) the timing of cellularization; and (iv) the process of the germ primordium formation. Between two and four micropyles lie in the mid-ventral region of the egg. The egg nucleus is at the mid-dorsal periphery of the new laid egg, and meiosis resumes and is completed there. The female pronucleus moves to the mid-ventral side, and fertilization occurs there. Energid starts to proliferate and migrates to the periphery of the egg, initiating blastoderm formation. Actin caps surround each superficial nucleus. Cellularization occurs during the blastoderm stage. At a late blastoderm stage, nuclei aggregate in both the posterolateral patch-like regions of the egg to form a germ primordium. The germ primordium looks like a pair of dumbbells. Both the patches shift towards the ventral side and fuse into a germ primordium. The germ primordium contracts to produce a clearly delineated germ band. Observations on distribution patterns of F-actin indicate that, all through the process, the germ primordium retains that unity, and is not separated into two parts.

Original languageEnglish
Pages (from-to)99-108
Number of pages10
JournalDevelopment Growth and Differentiation
Volume47
Issue number2
DOIs
Publication statusPublished - Feb 2005
Externally publishedYes

Fingerprint

Orthoptera
Gryllidae
Embryonic Development
Ovum
Insects
Blastoderm
Meiosis
Actins
Contracts
Fertilization
Electron Scanning Microscopy
Fluorescence
Staining and Labeling

Keywords

  • Embryogenesis
  • F-actin
  • Germ primordium
  • Gryllus bimaculatus
  • Micropyle

ASJC Scopus subject areas

  • Anatomy
  • Developmental Biology
  • Cell Biology

Cite this

Location of micropyles and early embryonic development of the two-spotted cricket Gryllus bimaculatus (Insecta, Orthoptera). / Sarashina, Isao; Mito, Taro; Saito, Michiko; Uneme, Hiroyuki; Miyawaki, Katsuyuki; Shinmyo, Yohei; Ohuchi, Hideyo; Noji, Sumihare.

In: Development Growth and Differentiation, Vol. 47, No. 2, 02.2005, p. 99-108.

Research output: Contribution to journalArticle

Sarashina, Isao ; Mito, Taro ; Saito, Michiko ; Uneme, Hiroyuki ; Miyawaki, Katsuyuki ; Shinmyo, Yohei ; Ohuchi, Hideyo ; Noji, Sumihare. / Location of micropyles and early embryonic development of the two-spotted cricket Gryllus bimaculatus (Insecta, Orthoptera). In: Development Growth and Differentiation. 2005 ; Vol. 47, No. 2. pp. 99-108.
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