Location of functional regions of acetylcholine receptor α-subunit by site-directed mutagenesis

Masayoshi Mishina, Takamasa Tobimatsu, Keiji Imoto, Ken Ichi Tanaka, Yoshihiko Fujita, Kazuhiko Fukuda, Masaaki Kurasaki, Hideo Takahashi, Yuuki Morimoto, Tadaaki Hirose, Seiichi Inayama, Tomoyuki Takahashi, Motoy Kuno, Shosaku Numa

Research output: Contribution to journalArticle

268 Citations (Scopus)

Abstract

The availability of cloned cDNAs encoding the four subunits of the Torpedo acetylcholine receptor, which can be expressed to make functional receptors in Xenopus oocytes, has made possible a detailed investigation of the functions of the different structural components of the receptor. The functional analysis of receptors with α-subunits altered at specific sites by site-directed mutagenesis of the cDNA has allowed the location of specific regions of the α-subunit molecule involved in acetylcholine binding and forming a transmembrane ionic channel.

Original languageEnglish
Pages (from-to)364-369
Number of pages6
JournalNature
Volume313
Issue number6001
DOIs
Publication statusPublished - Dec 1 1985
Externally publishedYes

ASJC Scopus subject areas

  • General

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