Locally produced estrogen promotes fetal rat metatarsal bone growth; an effect mediated through increased chondrocyte proliferation and decreased apoptosis

Andrei S. Chagin, D. Chrysis, Masaharu Takigawa, E. M. Ritzen, L. Sävendahl

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Abstract

The importance of estrogens for the regulation of longitudinal bone growth is unequivocal. However, any local effect of estrogens in growth plate cartilage has been debated. Recently, several enzymes essential for estrogen synthesis were shown to be expressed in rat growth plate chondrocytes. Local production of 17β-estradiol (E2) has also been demonstrated in rat costal chondrocytes. We aimed to determine the functional role of locally produced estrogen in growth plate cartilage. The human chondrocyte-like cell line HCS-2/8 was used to study estrogen effects on cell proliferation (3H-labeled thymidine uptake) and apoptosis (cell death detection ELISA kit). Chondrocyte production of E2 was measured by RIA and organ cultures of fetal rat metatarsal bones were used to study the effects of estrogen on longitudinal growth rate. We found that significant amounts of E2 were produced by HCS-2/8 chondrocytes (64.1 ± 5.3 fmol/3 days/106 cells). The aromatase inhibitor letrozole (1 μM) and the pure estrogen receptor antagonist ICI 182,780 (10 μM) inhibited proliferation of HCS-2/8 chondrocytes by 20% (P

Original languageEnglish
Pages (from-to)193-203
Number of pages11
JournalJournal of Endocrinology
Volume188
Issue number2
DOIs
Publication statusPublished - Feb 2006

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Metatarsal Bones
Bone Development
Chondrocytes
Estrogens
Apoptosis
Growth Plate
letrozole
Cartilage
Aromatase Inhibitors
Organ Culture Techniques
Thymidine
Estradiol
Cell Death
Enzyme-Linked Immunosorbent Assay
Cell Proliferation
Cell Line
Enzymes
Growth

ASJC Scopus subject areas

  • Endocrinology

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Locally produced estrogen promotes fetal rat metatarsal bone growth; an effect mediated through increased chondrocyte proliferation and decreased apoptosis. / Chagin, Andrei S.; Chrysis, D.; Takigawa, Masaharu; Ritzen, E. M.; Sävendahl, L.

In: Journal of Endocrinology, Vol. 188, No. 2, 02.2006, p. 193-203.

Research output: Contribution to journalArticle

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