Localization of type IV collagen alpha chains in tooth germ development

Naoki Katase, Hitoshi Nagatsuka, Keisuke Nakano, Ryo Tamamura, Noriyuki Nagai, Xie Wel-li, Yukiteru Matsuoka

Research output: Contribution to journalArticle

Abstract

The morphogenesis and morphological development of molar are regulated by the interaction between odontogenic epithelium and mesenchymal tissues. And Type IV collagen molecules, the major component of the dental basement membrane, are composed of three alpha chains. Using indirect immunofluorescence on cryosections, we characterized the changes in protein localization of alpha 1 to 6 chains of type IV collagen during molar development in mice molar and human tooth germ. ICR mouse embryos (detection of vaginal plug: day 0) and newborn mice were used. Human tooth germ was kindly provided by Dr. Yamamoto of Ohu University. Rat monoclonal antibodies recognizing type IV collagen alpha chains were raised against synthetic peptides of non-consensus amino acid sequences of the human and mice alpha (IV) chains. These monoclonal antibodies were kindly provided by Shigei medical research institute. Both mice and human tooth germ, we detected conversions in localization and composition of type IV collagen alpha chains working with tooth germ development. These results imply that there are time and site specificity, which may be involved in tooth morphogenesis and the tooth germ development, in type IV collagen alpha chains.

Original languageEnglish
Pages (from-to)124-125
Number of pages2
JournalJournal of Hard Tissue Biology
Volume14
Issue number2
DOIs
Publication statusPublished - 2005

Fingerprint

Tooth Germ
Collagen Type IV
Collagen
Monoclonal antibodies
Morphogenesis
Tooth
Monoclonal Antibodies
Inbred ICR Mouse
Indirect Fluorescent Antibody Technique
Basement Membrane
Peptides
Biomedical Research
Amino acids
Rats
Amino Acid Sequence
Embryonic Structures
Epithelium
Tissue
Proteins
Amino Acids

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry
  • Biomaterials
  • Orthopedics and Sports Medicine
  • Dentistry(all)
  • Cell Biology

Cite this

Localization of type IV collagen alpha chains in tooth germ development. / Katase, Naoki; Nagatsuka, Hitoshi; Nakano, Keisuke; Tamamura, Ryo; Nagai, Noriyuki; Wel-li, Xie; Matsuoka, Yukiteru.

In: Journal of Hard Tissue Biology, Vol. 14, No. 2, 2005, p. 124-125.

Research output: Contribution to journalArticle

Katase, Naoki ; Nagatsuka, Hitoshi ; Nakano, Keisuke ; Tamamura, Ryo ; Nagai, Noriyuki ; Wel-li, Xie ; Matsuoka, Yukiteru. / Localization of type IV collagen alpha chains in tooth germ development. In: Journal of Hard Tissue Biology. 2005 ; Vol. 14, No. 2. pp. 124-125.
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