Localization of type IV collagen α 1 to α 6 chains in basement: Membrane during mouse molar germ development

N. Nagai, Keisuke Nakano, Y. Sado, I. Naito, M. Gunduz, H. Tsujigiwa, Hitoshi Nagatsuka, Y. Ninomiya, C. H. Siar

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The dental basement membrane (BM) putatively mediates epithelial-mesenchymal interactions during tooth morphogenesis and cytodifferentiation. Type IV collagen α chains, a major network-forming protein of the dental BM, was studied and results disclosed distinct expression patterns at different stages of mouse molar germ development. At the dental placode and bud stage, the BM of the oral epithelium expressed α 1, α 2, α 5 and α 6 chains while the gubernaculum dentis, in addition to the above four chains, also expressed α 4 chain. An asymmetrical expression for α 4, α 5 and α 6 chains was observed at the bud stage. At the early bell stage, the BM associated with the inner enamel epithelium (IEE) of molar germ expressed α 1, α 2 and α 4 chains while the BM of the outer enamel epithelium (OEE) expressed only α 1 and α 2 chains. With the onset of dentinogenesis, the collagen α chain profile of the IEE BM gradually disappeared. However from the early to late bell stage, the gubernaculum dentis consistently expressed α 1, α 2, α 5 and α 6 chains resembling fetal oral mucosa. These findings suggest that stage- and position- specific distribution of type IV collagen α subunits occur during molar germ development and that these changes are essential for molar morphogenesis and cytodifferentiation.

Original languageEnglish
Pages (from-to)827-831
Number of pages5
JournalInternational Journal of Developmental Biology
Volume45
Issue number7
Publication statusPublished - 2001

Fingerprint

Collagen Type I
Basement Membrane
Tooth
Dental Enamel
Epithelium
Collagen Type IV
Morphogenesis
Dentinogenesis
Mouth Mucosa
Collagen
Proteins

Keywords

  • α chains
  • Basement membrane
  • Immunofluorescence localization
  • Molar germ development
  • Type IV collagen

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Localization of type IV collagen α 1 to α 6 chains in basement : Membrane during mouse molar germ development. / Nagai, N.; Nakano, Keisuke; Sado, Y.; Naito, I.; Gunduz, M.; Tsujigiwa, H.; Nagatsuka, Hitoshi; Ninomiya, Y.; Siar, C. H.

In: International Journal of Developmental Biology, Vol. 45, No. 7, 2001, p. 827-831.

Research output: Contribution to journalArticle

Nagai, N. ; Nakano, Keisuke ; Sado, Y. ; Naito, I. ; Gunduz, M. ; Tsujigiwa, H. ; Nagatsuka, Hitoshi ; Ninomiya, Y. ; Siar, C. H. / Localization of type IV collagen α 1 to α 6 chains in basement : Membrane during mouse molar germ development. In: International Journal of Developmental Biology. 2001 ; Vol. 45, No. 7. pp. 827-831.
@article{949f203cdd034b7786e5de8488798b67,
title = "Localization of type IV collagen α 1 to α 6 chains in basement: Membrane during mouse molar germ development",
abstract = "The dental basement membrane (BM) putatively mediates epithelial-mesenchymal interactions during tooth morphogenesis and cytodifferentiation. Type IV collagen α chains, a major network-forming protein of the dental BM, was studied and results disclosed distinct expression patterns at different stages of mouse molar germ development. At the dental placode and bud stage, the BM of the oral epithelium expressed α 1, α 2, α 5 and α 6 chains while the gubernaculum dentis, in addition to the above four chains, also expressed α 4 chain. An asymmetrical expression for α 4, α 5 and α 6 chains was observed at the bud stage. At the early bell stage, the BM associated with the inner enamel epithelium (IEE) of molar germ expressed α 1, α 2 and α 4 chains while the BM of the outer enamel epithelium (OEE) expressed only α 1 and α 2 chains. With the onset of dentinogenesis, the collagen α chain profile of the IEE BM gradually disappeared. However from the early to late bell stage, the gubernaculum dentis consistently expressed α 1, α 2, α 5 and α 6 chains resembling fetal oral mucosa. These findings suggest that stage- and position- specific distribution of type IV collagen α subunits occur during molar germ development and that these changes are essential for molar morphogenesis and cytodifferentiation.",
keywords = "α chains, Basement membrane, Immunofluorescence localization, Molar germ development, Type IV collagen",
author = "N. Nagai and Keisuke Nakano and Y. Sado and I. Naito and M. Gunduz and H. Tsujigiwa and Hitoshi Nagatsuka and Y. Ninomiya and Siar, {C. H.}",
year = "2001",
language = "English",
volume = "45",
pages = "827--831",
journal = "International Journal of Developmental Biology",
issn = "0214-6282",
publisher = "University of the Basque Country Press",
number = "7",

}

TY - JOUR

T1 - Localization of type IV collagen α 1 to α 6 chains in basement

T2 - Membrane during mouse molar germ development

AU - Nagai, N.

AU - Nakano, Keisuke

AU - Sado, Y.

AU - Naito, I.

AU - Gunduz, M.

AU - Tsujigiwa, H.

AU - Nagatsuka, Hitoshi

AU - Ninomiya, Y.

AU - Siar, C. H.

PY - 2001

Y1 - 2001

N2 - The dental basement membrane (BM) putatively mediates epithelial-mesenchymal interactions during tooth morphogenesis and cytodifferentiation. Type IV collagen α chains, a major network-forming protein of the dental BM, was studied and results disclosed distinct expression patterns at different stages of mouse molar germ development. At the dental placode and bud stage, the BM of the oral epithelium expressed α 1, α 2, α 5 and α 6 chains while the gubernaculum dentis, in addition to the above four chains, also expressed α 4 chain. An asymmetrical expression for α 4, α 5 and α 6 chains was observed at the bud stage. At the early bell stage, the BM associated with the inner enamel epithelium (IEE) of molar germ expressed α 1, α 2 and α 4 chains while the BM of the outer enamel epithelium (OEE) expressed only α 1 and α 2 chains. With the onset of dentinogenesis, the collagen α chain profile of the IEE BM gradually disappeared. However from the early to late bell stage, the gubernaculum dentis consistently expressed α 1, α 2, α 5 and α 6 chains resembling fetal oral mucosa. These findings suggest that stage- and position- specific distribution of type IV collagen α subunits occur during molar germ development and that these changes are essential for molar morphogenesis and cytodifferentiation.

AB - The dental basement membrane (BM) putatively mediates epithelial-mesenchymal interactions during tooth morphogenesis and cytodifferentiation. Type IV collagen α chains, a major network-forming protein of the dental BM, was studied and results disclosed distinct expression patterns at different stages of mouse molar germ development. At the dental placode and bud stage, the BM of the oral epithelium expressed α 1, α 2, α 5 and α 6 chains while the gubernaculum dentis, in addition to the above four chains, also expressed α 4 chain. An asymmetrical expression for α 4, α 5 and α 6 chains was observed at the bud stage. At the early bell stage, the BM associated with the inner enamel epithelium (IEE) of molar germ expressed α 1, α 2 and α 4 chains while the BM of the outer enamel epithelium (OEE) expressed only α 1 and α 2 chains. With the onset of dentinogenesis, the collagen α chain profile of the IEE BM gradually disappeared. However from the early to late bell stage, the gubernaculum dentis consistently expressed α 1, α 2, α 5 and α 6 chains resembling fetal oral mucosa. These findings suggest that stage- and position- specific distribution of type IV collagen α subunits occur during molar germ development and that these changes are essential for molar morphogenesis and cytodifferentiation.

KW - α chains

KW - Basement membrane

KW - Immunofluorescence localization

KW - Molar germ development

KW - Type IV collagen

UR - http://www.scopus.com/inward/record.url?scp=0035185425&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035185425&partnerID=8YFLogxK

M3 - Article

C2 - 11732842

AN - SCOPUS:0035185425

VL - 45

SP - 827

EP - 831

JO - International Journal of Developmental Biology

JF - International Journal of Developmental Biology

SN - 0214-6282

IS - 7

ER -