Localization of receptors for atrial natriuretic polypeptide (ANP) in the glomerulus: in vitro electron microscopic autoradiographical investigation using 125I-labeled ANP

Kiminao Mizukawa, Toshiro Ogura, Ichiro Yamamoto, Takeshi Mitsui, Eriko Katayama, Zensuke Ota, Norio Ogawa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The localization of receptors for atrial natriuretic polypeptide (ANP) in the glomerulus of the rat was studied by electron microscopic autoradiography using 125I-labeled ANP. A total of 1,134 silver grains counted in 30 glomeruli were distributed as follows: processes of the podocytes (36.4%), cell bodies of the podocytes (14.8%), basement membrane (12.3%), endothelial cells (6.3%), mesangial cells (7.7%) and others, for example the vascular spaces (22.5%). This finding indicates that ANP binding sites are mainly localized on the foot processes of the podocytes in the glomerulus.

Original languageEnglish
Pages (from-to)167-172
Number of pages6
JournalRegulatory Peptides
Volume21
Issue number1-2
DOIs
Publication statusPublished - 1988

Fingerprint

Podocytes
Electrons
Peptides
Mesangial Cells
Endothelial cells
Autoradiography
Silver
Basement Membrane
Blood Vessels
Rats
Foot
Endothelial Cells
Binding Sites
Cells
In Vitro Techniques

Keywords

  • Atrial natriuretic polypeptide receptor
  • Glomerulus
  • In vitro autoradiography
  • Podocyte
  • Ultrastructure

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Neuroscience(all)

Cite this

Localization of receptors for atrial natriuretic polypeptide (ANP) in the glomerulus : in vitro electron microscopic autoradiographical investigation using 125I-labeled ANP. / Mizukawa, Kiminao; Ogura, Toshiro; Yamamoto, Ichiro; Mitsui, Takeshi; Katayama, Eriko; Ota, Zensuke; Ogawa, Norio.

In: Regulatory Peptides, Vol. 21, No. 1-2, 1988, p. 167-172.

Research output: Contribution to journalArticle

Mizukawa, Kiminao ; Ogura, Toshiro ; Yamamoto, Ichiro ; Mitsui, Takeshi ; Katayama, Eriko ; Ota, Zensuke ; Ogawa, Norio. / Localization of receptors for atrial natriuretic polypeptide (ANP) in the glomerulus : in vitro electron microscopic autoradiographical investigation using 125I-labeled ANP. In: Regulatory Peptides. 1988 ; Vol. 21, No. 1-2. pp. 167-172.
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