Localization of receptors for atrial natriuretic polypeptide (ANP) in the glomerulus: in vitro electron microscopic autoradiographical investigation using 125I-labeled ANP

Kiminao Mizukawa; Toshiro Ogura; Ichiro Yamamoto; Takeshi Mitsui; Eriko Katayama; Zensuke Ota; Norio Ogawa

doi:10.1016/0167-0115(88)90100-0

Localization of receptors for atrial natriuretic polypeptide (ANP) in the glomerulus: in vitro electron microscopic autoradiographical investigation using ¹²⁵I-labeled ANP

Kiminao Mizukawa, Toshiro Ogura, Ichiro Yamamoto, Takeshi Mitsui, Eriko Katayama, Zensuke Ota, Norio Ogawa

Health Service Center

Research output: Contribution to journal › Article › peer-review

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Abstract

The localization of receptors for atrial natriuretic polypeptide (ANP) in the glomerulus of the rat was studied by electron microscopic autoradiography using ¹²⁵I-labeled ANP. A total of 1,134 silver grains counted in 30 glomeruli were distributed as follows: processes of the podocytes (36.4%), cell bodies of the podocytes (14.8%), basement membrane (12.3%), endothelial cells (6.3%), mesangial cells (7.7%) and others, for example the vascular spaces (22.5%). This finding indicates that ANP binding sites are mainly localized on the foot processes of the podocytes in the glomerulus.

Original language	English
Pages (from-to)	167-172
Number of pages	6
Journal	Regulatory Peptides
Volume	21
Issue number	1-2
DOIs	https://doi.org/10.1016/0167-0115(88)90100-0
Publication status	Published - May 1988

Keywords

Atrial natriuretic polypeptide receptor
Glomerulus
In vitro autoradiography
Podocyte
Ultrastructure

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Clinical Biochemistry
Cellular and Molecular Neuroscience

Access to Document

10.1016/0167-0115(88)90100-0

Cite this

@article{9f9555daf866410aba5f06eb10558c86,

title = "Localization of receptors for atrial natriuretic polypeptide (ANP) in the glomerulus: in vitro electron microscopic autoradiographical investigation using 125I-labeled ANP",

abstract = "The localization of receptors for atrial natriuretic polypeptide (ANP) in the glomerulus of the rat was studied by electron microscopic autoradiography using 125I-labeled ANP. A total of 1,134 silver grains counted in 30 glomeruli were distributed as follows: processes of the podocytes (36.4%), cell bodies of the podocytes (14.8%), basement membrane (12.3%), endothelial cells (6.3%), mesangial cells (7.7%) and others, for example the vascular spaces (22.5%). This finding indicates that ANP binding sites are mainly localized on the foot processes of the podocytes in the glomerulus.",

keywords = "Atrial natriuretic polypeptide receptor, Glomerulus, In vitro autoradiography, Podocyte, Ultrastructure",

author = "Kiminao Mizukawa and Toshiro Ogura and Ichiro Yamamoto and Takeshi Mitsui and Eriko Katayama and Zensuke Ota and Norio Ogawa",

note = "Funding Information: This work was supported in part by research grants from the Ministry of Education, Science and Culture, and the Ministry of Health and Welfare of the Japanese Government.",

year = "1988",

month = may,

doi = "10.1016/0167-0115(88)90100-0",

language = "English",

volume = "21",

pages = "167--172",

journal = "Regulatory Peptides",

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publisher = "Elsevier",

number = "1-2",

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T1 - Localization of receptors for atrial natriuretic polypeptide (ANP) in the glomerulus

T2 - in vitro electron microscopic autoradiographical investigation using 125I-labeled ANP

AU - Mizukawa, Kiminao

AU - Ogura, Toshiro

AU - Yamamoto, Ichiro

AU - Mitsui, Takeshi

AU - Katayama, Eriko

AU - Ota, Zensuke

AU - Ogawa, Norio

N1 - Funding Information: This work was supported in part by research grants from the Ministry of Education, Science and Culture, and the Ministry of Health and Welfare of the Japanese Government.

PY - 1988/5

Y1 - 1988/5

N2 - The localization of receptors for atrial natriuretic polypeptide (ANP) in the glomerulus of the rat was studied by electron microscopic autoradiography using 125I-labeled ANP. A total of 1,134 silver grains counted in 30 glomeruli were distributed as follows: processes of the podocytes (36.4%), cell bodies of the podocytes (14.8%), basement membrane (12.3%), endothelial cells (6.3%), mesangial cells (7.7%) and others, for example the vascular spaces (22.5%). This finding indicates that ANP binding sites are mainly localized on the foot processes of the podocytes in the glomerulus.

AB - The localization of receptors for atrial natriuretic polypeptide (ANP) in the glomerulus of the rat was studied by electron microscopic autoradiography using 125I-labeled ANP. A total of 1,134 silver grains counted in 30 glomeruli were distributed as follows: processes of the podocytes (36.4%), cell bodies of the podocytes (14.8%), basement membrane (12.3%), endothelial cells (6.3%), mesangial cells (7.7%) and others, for example the vascular spaces (22.5%). This finding indicates that ANP binding sites are mainly localized on the foot processes of the podocytes in the glomerulus.

KW - Atrial natriuretic polypeptide receptor

KW - Glomerulus

KW - In vitro autoradiography

KW - Podocyte

KW - Ultrastructure

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