Localization of Rab5 to synaptic vesicles identifies endosomal intermediate in synaptic vesicle recycling pathway

G. Fischer von Mollard, B. Stahl, C. Walch-Solimena, K. Takei, L. Daniels, A. Khoklatchev, P. De Camilli, T. C. Sudhof, R. Jahn

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

After exocytosis, synaptic vesicles rapidly endocytose and recycle but little is known about the molecular mechanisms involved. Rab5 is a ubiquitous low molecular weight GTP-binding protein required for endosomal fusion in fibroblasts. We have now raised polyclonal and monoclonal antibodies to rat Rab5 and show that in rat brain, Rab5 is a major synaptic vesicle protein. Immunoisolation of vesicular organelles from brain with antibodies to either Rab3A and Rab5 as small GTP-binding proteins or with synaptophysin as general synaptic vesicle marker demonstrates that there are overlapping populations of synaptic vesicles containing either Rab5 or Rab3A or both, suggesting a stage-specific association of these low-molecular weight GTP-binding proteins with synaptic vesicles. Our data provide the first biochemical evidence that synaptic vesicle recycling involves an endosomal intermediate similar to that of the receptor-mediated endocytosis pathway.

Original languageEnglish
Pages (from-to)319-326
Number of pages8
JournalEuropean Journal of Cell Biology
Volume65
Issue number2
Publication statusPublished - Jan 1 1994
Externally publishedYes

Keywords

  • Endocytosis
  • Endosomes
  • Exocytosis
  • Small GTP binding proteins
  • Synaptic vesicles

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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    Fischer von Mollard, G., Stahl, B., Walch-Solimena, C., Takei, K., Daniels, L., Khoklatchev, A., De Camilli, P., Sudhof, T. C., & Jahn, R. (1994). Localization of Rab5 to synaptic vesicles identifies endosomal intermediate in synaptic vesicle recycling pathway. European Journal of Cell Biology, 65(2), 319-326.