Localization of prolidase gene expression in scar tissue using in situ hybridization

Yasuko Senboshi, Takashi Oono, Jirô Arata

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We investigated prolidase gene expression in human skin by means of Northern blot analysis and in situ hybridization. Northern blot analysis revealed that an mRNA species that was specific for prolidase was present in cultured human skin fibroblasts and keratinocytes. In situ hybridization using non-isotopic riboprobes labeled with digoxigenin and an isotopic riboprobe labeled with [35S]UTP localized prolidase gene expression to fibroblasts and endothelial cells of small vessels in scar tissue. Prolidase mRNA was also prominently expressed in keratinocytes near the basal layer overlying scar tissue. These findings indicate that prolidase may have an important role in wound healing.

Original languageEnglish
Pages (from-to)163-171
Number of pages9
JournalJournal of Dermatological Science
Volume12
Issue number2
DOIs
Publication statusPublished - Jan 1 1996

Fingerprint

proline dipeptidase
Gene expression
Cicatrix
In Situ Hybridization
Tissue
Gene Expression
Fibroblasts
Keratinocytes
Northern Blotting
Skin
Digoxigenin
Messenger RNA
Uridine Triphosphate
Endothelial cells
Wound Healing
Endothelial Cells

Keywords

  • In situ hybridization
  • Prolidase
  • Scar tissue
  • Wound healing

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

Localization of prolidase gene expression in scar tissue using in situ hybridization. / Senboshi, Yasuko; Oono, Takashi; Arata, Jirô.

In: Journal of Dermatological Science, Vol. 12, No. 2, 01.01.1996, p. 163-171.

Research output: Contribution to journalArticle

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