Localization of N-ethylmaleimide-sensitive fusion protein in pinealocytes

Yoshinori Moriyama, Akitsugu Yamamoto, Mitsuo Tagaya, Yutaka Tashiro, Hitoshi Michibata

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

N-ETHYLMALElMiDE-sensitive fusion protein (NSF), a protein necessary for vesicular docking and/or fusion, was detected immunohistochemically in pinealocytes. NSF was distributed similarly to synaptophysin and vacuolar-type H+-ATPase (V-ATPase), marker proteins for synaptic-like microvesicles (MVs) abundantly present in pinealocytes. A subcellular fractionation study indicated that >95% of NSF was present as a membrane-bound form and that some NSF was associated with MVs. Like neuronal NSF, the protein was not solubilized from membranes with either 2mM Mg-ATP or 2% sodium carbonate, suggesting that NSF was tightly bound to the membranes. NSF was also detected in purified MVs from bovine posterior pituitaries. Since MVs are the organelles in which transmitters are stored, these results suggest that NSF is involved in the MV-mediated exocytosis of transmitters from endocrine cells.

Original languageEnglish
Pages (from-to)1757-1760
Number of pages4
JournalNeuroReport
Volume6
Issue number13
DOIs
Publication statusPublished - Sep 1995
Externally publishedYes

Keywords

  • Chemical transduction
  • Chromaffin granules
  • Exocytosis
  • Micro-vesicles
  • NEM-sensitive fusion protein (NSF)
  • Pineal gland
  • Pinealocytes
  • Posterior pituitary
  • Synaptic vesicles
  • Vesicular transport

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Localization of N-ethylmaleimide-sensitive fusion protein in pinealocytes'. Together they form a unique fingerprint.

  • Cite this

    Moriyama, Y., Yamamoto, A., Tagaya, M., Tashiro, Y., & Michibata, H. (1995). Localization of N-ethylmaleimide-sensitive fusion protein in pinealocytes. NeuroReport, 6(13), 1757-1760. https://doi.org/10.1097/00001756-199509000-00012