TY - JOUR
T1 - Localization of matrix metalloproteinases (MMPs-2, 8, 9 and 20) in normal and carious dentine
AU - Shimada, Y.
AU - Ichinose, S.
AU - Sadr, A.
AU - Burrow, M. F.
AU - Tagami, J.
PY - 2009/12
Y1 - 2009/12
N2 - Background: Dentine matrix metalloproteinases (MMPs) may participate in the destruction of dentine following demineralization by bacterial acids. This study investigated the localization of MMPs in carious dentine. Methods: Frozen sections of dentine caries were prepared without demineralization and immersed in monoclonal antibody against MMP-2, -8, -9 and -20. The sections were labelled by IgG conjugated with gold colloidal particles, and observed under FE-SEM. Labelling indexes (number of gold particles /μm2) of outer and inner carious dentine, respectively, with and without bacterial infection, were compared with that of normal dentine. Results: MMP-2 was distributed in both carious and normal dentine; the level of MMP-2 showed no significant difference among the outer caries, inner caries, and normal dentine. The labelling indexes of MMP-8 and MMP-9 both significantly decreased at the inner carious dentine compared with the level of normal dentine, but intensified again at the outer caries region. The labelling index of MMP-20 was the highest at normal dentine. Conclusions: The localization of MMPs was visibly detected using immunogold labelling. The localization of MMP-2 showed no significant difference among the three regions, while MMP-8 and MMP-9 showed significant reduction at the inner caries layer, and MMP-20 reduced toward the outer caries.
AB - Background: Dentine matrix metalloproteinases (MMPs) may participate in the destruction of dentine following demineralization by bacterial acids. This study investigated the localization of MMPs in carious dentine. Methods: Frozen sections of dentine caries were prepared without demineralization and immersed in monoclonal antibody against MMP-2, -8, -9 and -20. The sections were labelled by IgG conjugated with gold colloidal particles, and observed under FE-SEM. Labelling indexes (number of gold particles /μm2) of outer and inner carious dentine, respectively, with and without bacterial infection, were compared with that of normal dentine. Results: MMP-2 was distributed in both carious and normal dentine; the level of MMP-2 showed no significant difference among the outer caries, inner caries, and normal dentine. The labelling indexes of MMP-8 and MMP-9 both significantly decreased at the inner carious dentine compared with the level of normal dentine, but intensified again at the outer caries region. The labelling index of MMP-20 was the highest at normal dentine. Conclusions: The localization of MMPs was visibly detected using immunogold labelling. The localization of MMP-2 showed no significant difference among the three regions, while MMP-8 and MMP-9 showed significant reduction at the inner caries layer, and MMP-20 reduced toward the outer caries.
KW - Carious dentine
KW - Immunogold labelling
KW - Inner caries
KW - Matrix metalloproteinases
KW - Outer caries
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U2 - 10.1111/j.1834-7819.2009.01161.x
DO - 10.1111/j.1834-7819.2009.01161.x
M3 - Article
C2 - 20415934
AN - SCOPUS:77949349586
SN - 0045-0421
VL - 54
SP - 347
EP - 354
JO - Australian Dental Journal
JF - Australian Dental Journal
IS - 4
ER -