Localization of matrix metalloproteinases (MMPs-2, 8, 9 and 20) in normal and carious dentine

Y. Shimada, S. Ichinose, A. Sadr, M. F. Burrow, J. Tagami

Research output: Contribution to journalArticlepeer-review

64 Citations (Scopus)


Background: Dentine matrix metalloproteinases (MMPs) may participate in the destruction of dentine following demineralization by bacterial acids. This study investigated the localization of MMPs in carious dentine. Methods: Frozen sections of dentine caries were prepared without demineralization and immersed in monoclonal antibody against MMP-2, -8, -9 and -20. The sections were labelled by IgG conjugated with gold colloidal particles, and observed under FE-SEM. Labelling indexes (number of gold particles /μm2) of outer and inner carious dentine, respectively, with and without bacterial infection, were compared with that of normal dentine. Results: MMP-2 was distributed in both carious and normal dentine; the level of MMP-2 showed no significant difference among the outer caries, inner caries, and normal dentine. The labelling indexes of MMP-8 and MMP-9 both significantly decreased at the inner carious dentine compared with the level of normal dentine, but intensified again at the outer caries region. The labelling index of MMP-20 was the highest at normal dentine. Conclusions: The localization of MMPs was visibly detected using immunogold labelling. The localization of MMP-2 showed no significant difference among the three regions, while MMP-8 and MMP-9 showed significant reduction at the inner caries layer, and MMP-20 reduced toward the outer caries.

Original languageEnglish
Pages (from-to)347-354
Number of pages8
JournalAustralian Dental Journal
Issue number4
Publication statusPublished - Dec 2009
Externally publishedYes


  • Carious dentine
  • Immunogold labelling
  • Inner caries
  • Matrix metalloproteinases
  • Outer caries

ASJC Scopus subject areas

  • Dentistry(all)


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