Localization of HTLV-I-associated antigens in adult T cell leukemia cells and HTLV-I-infected cell line cells

F. X. Jin, K. Iwatsuki, D. Horiguchi, K. Sugaya, F. Nakayama, M. Koide, M. Takigawa, M. Yamada

Research output: Contribution to journalArticle

Abstract

Localization of HTLV-I-associated antigens was studied in adult T cell leukemia (ATL) cells and HTLV-I-infected cell line cells using monoclonal and human polyclonal antibodies against the viral-related antigens. Two monoclonal antibodies that we obtained by hybridoma technique reacted with HTLV-I-virus core antigens, P19 and P24, respectively. Human anti-HTLV-I-antibodies, which were purified from sera from ATL patients reacted with not only HTLV-I virus particles but also their precursors located in the cytoplasm. In tumor cells freshly isolated from ATL patients, no expression of the virus antigens was observed. When the cells were cultured for several days, the virus antigens were defined in about 3-5% of the cultured cells by the monoclonal antibodies, and in 5-10% by the purified human anti-HTLV-I antibodies. Addition of 5-iodo-2'-deoxyuridine to the culture inhibited cell growth, and at the same time, increased the percentage of the virus antigen-positive cells. Established HTLV-I-infected cell lines showed different cytological profiles from the original ATL cells in the viral replication and morphology.

Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalNippon Hifuka Gakkai zasshi. The Japanese journal of dermatology
Volume101
Issue number2
Publication statusPublished - Feb 1991
Externally publishedYes

Fingerprint

HTLV-I Antigens
Adult T Cell Leukemia Lymphoma
Human T-lymphotropic virus 1
Cell Line
HTLV-I Antibodies
Deltaretrovirus
Antigens
Viruses
Cultured Cells
Monoclonal Antibodies
Idoxuridine
Viral Antigens
Hybridomas
Virion
Cytoplasm
Cell Culture Techniques
Antibodies
Growth
Serum

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Jin, F. X., Iwatsuki, K., Horiguchi, D., Sugaya, K., Nakayama, F., Koide, M., ... Yamada, M. (1991). Localization of HTLV-I-associated antigens in adult T cell leukemia cells and HTLV-I-infected cell line cells. Nippon Hifuka Gakkai zasshi. The Japanese journal of dermatology, 101(2), 89-95.

Localization of HTLV-I-associated antigens in adult T cell leukemia cells and HTLV-I-infected cell line cells. / Jin, F. X.; Iwatsuki, K.; Horiguchi, D.; Sugaya, K.; Nakayama, F.; Koide, M.; Takigawa, M.; Yamada, M.

In: Nippon Hifuka Gakkai zasshi. The Japanese journal of dermatology, Vol. 101, No. 2, 02.1991, p. 89-95.

Research output: Contribution to journalArticle

Jin, FX, Iwatsuki, K, Horiguchi, D, Sugaya, K, Nakayama, F, Koide, M, Takigawa, M & Yamada, M 1991, 'Localization of HTLV-I-associated antigens in adult T cell leukemia cells and HTLV-I-infected cell line cells', Nippon Hifuka Gakkai zasshi. The Japanese journal of dermatology, vol. 101, no. 2, pp. 89-95.
Jin, F. X. ; Iwatsuki, K. ; Horiguchi, D. ; Sugaya, K. ; Nakayama, F. ; Koide, M. ; Takigawa, M. ; Yamada, M. / Localization of HTLV-I-associated antigens in adult T cell leukemia cells and HTLV-I-infected cell line cells. In: Nippon Hifuka Gakkai zasshi. The Japanese journal of dermatology. 1991 ; Vol. 101, No. 2. pp. 89-95.
@article{0182bd659dad4891bf27fb5b0ab00cbe,
title = "Localization of HTLV-I-associated antigens in adult T cell leukemia cells and HTLV-I-infected cell line cells",
abstract = "Localization of HTLV-I-associated antigens was studied in adult T cell leukemia (ATL) cells and HTLV-I-infected cell line cells using monoclonal and human polyclonal antibodies against the viral-related antigens. Two monoclonal antibodies that we obtained by hybridoma technique reacted with HTLV-I-virus core antigens, P19 and P24, respectively. Human anti-HTLV-I-antibodies, which were purified from sera from ATL patients reacted with not only HTLV-I virus particles but also their precursors located in the cytoplasm. In tumor cells freshly isolated from ATL patients, no expression of the virus antigens was observed. When the cells were cultured for several days, the virus antigens were defined in about 3-5{\%} of the cultured cells by the monoclonal antibodies, and in 5-10{\%} by the purified human anti-HTLV-I antibodies. Addition of 5-iodo-2'-deoxyuridine to the culture inhibited cell growth, and at the same time, increased the percentage of the virus antigen-positive cells. Established HTLV-I-infected cell lines showed different cytological profiles from the original ATL cells in the viral replication and morphology.",
author = "Jin, {F. X.} and K. Iwatsuki and D. Horiguchi and K. Sugaya and F. Nakayama and M. Koide and M. Takigawa and M. Yamada",
year = "1991",
month = "2",
language = "English",
volume = "101",
pages = "89--95",
journal = "Nippon Hifuka Gakkai zasshi. The Japanese journal of dermatology",
issn = "0021-499X",
number = "2",

}

TY - JOUR

T1 - Localization of HTLV-I-associated antigens in adult T cell leukemia cells and HTLV-I-infected cell line cells

AU - Jin, F. X.

AU - Iwatsuki, K.

AU - Horiguchi, D.

AU - Sugaya, K.

AU - Nakayama, F.

AU - Koide, M.

AU - Takigawa, M.

AU - Yamada, M.

PY - 1991/2

Y1 - 1991/2

N2 - Localization of HTLV-I-associated antigens was studied in adult T cell leukemia (ATL) cells and HTLV-I-infected cell line cells using monoclonal and human polyclonal antibodies against the viral-related antigens. Two monoclonal antibodies that we obtained by hybridoma technique reacted with HTLV-I-virus core antigens, P19 and P24, respectively. Human anti-HTLV-I-antibodies, which were purified from sera from ATL patients reacted with not only HTLV-I virus particles but also their precursors located in the cytoplasm. In tumor cells freshly isolated from ATL patients, no expression of the virus antigens was observed. When the cells were cultured for several days, the virus antigens were defined in about 3-5% of the cultured cells by the monoclonal antibodies, and in 5-10% by the purified human anti-HTLV-I antibodies. Addition of 5-iodo-2'-deoxyuridine to the culture inhibited cell growth, and at the same time, increased the percentage of the virus antigen-positive cells. Established HTLV-I-infected cell lines showed different cytological profiles from the original ATL cells in the viral replication and morphology.

AB - Localization of HTLV-I-associated antigens was studied in adult T cell leukemia (ATL) cells and HTLV-I-infected cell line cells using monoclonal and human polyclonal antibodies against the viral-related antigens. Two monoclonal antibodies that we obtained by hybridoma technique reacted with HTLV-I-virus core antigens, P19 and P24, respectively. Human anti-HTLV-I-antibodies, which were purified from sera from ATL patients reacted with not only HTLV-I virus particles but also their precursors located in the cytoplasm. In tumor cells freshly isolated from ATL patients, no expression of the virus antigens was observed. When the cells were cultured for several days, the virus antigens were defined in about 3-5% of the cultured cells by the monoclonal antibodies, and in 5-10% by the purified human anti-HTLV-I antibodies. Addition of 5-iodo-2'-deoxyuridine to the culture inhibited cell growth, and at the same time, increased the percentage of the virus antigen-positive cells. Established HTLV-I-infected cell lines showed different cytological profiles from the original ATL cells in the viral replication and morphology.

UR - http://www.scopus.com/inward/record.url?scp=0026113566&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026113566&partnerID=8YFLogxK

M3 - Article

C2 - 2072580

AN - SCOPUS:0026113566

VL - 101

SP - 89

EP - 95

JO - Nippon Hifuka Gakkai zasshi. The Japanese journal of dermatology

JF - Nippon Hifuka Gakkai zasshi. The Japanese journal of dermatology

SN - 0021-499X

IS - 2

ER -