Localization of gene and protein expressions of tumor necrosis factor-α and tumor necrosis factor receptor types I and II in the bovine corpus luteum during the estrous cycle

R. Sakumoto, M. Vermehren, R. A. Kenngott, K. Okuda, F. Sinowatz

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

One of the many roles of tumor necrosis factor (TNF)-α is to control mammalian corpus luteum (CL) PG synthesis and apoptotic cell death. Here, the cellular localization of TNF-α and its type I (TNF-RI) and type II (TNF-RII) receptors in bovine luteal tissue were analyzed using in situ hybridization, immunohistochemistry, and quantitative realtime PCR. Transcripts for TNF-α were expressed in bovine CL throughout the estrous cycle, but were significantly more abundant (P <0.01) at the regressed luteal stage than at the other stages. Localization of TNF-α transcripts and protein were observed in large and small bovine luteal cells, as well as in immune cells. Moreover, transcripts for TNF-RI and TNF-RII were expressed in bovine CL throughout the estrous cycle. The abundance of TNF-RII transcripts was greater (P <0.01) at the regressed luteal stage than at the other stages, whereas TNF-RI transcript abundance did not significantly change. Expression of TNF-RI and TNFRII transcripts and proteins were observed in both the large and small luteal cells, and the proteins were also expressed in the immune cells and vascular endothelial cells. These results suggest that TNF-α sources include immune cells, as well as large and small luteal cells, and that TNF-RI and TNF-RII are present in the luteal cells of the bovine CL.

Original languageEnglish
Pages (from-to)3040-3047
Number of pages8
JournalJournal of Animal Science
Volume89
Issue number10
DOIs
Publication statusPublished - Oct 2011

Fingerprint

Receptors, Tumor Necrosis Factor, Type II
Receptors, Tumor Necrosis Factor, Type I
Estrous Cycle
tumor necrosis factors
Corpus Luteum
corpus luteum
estrous cycle
Tumor Necrosis Factor-alpha
protein synthesis
Luteal Cells
Gene Expression
gene expression
receptors
cattle
Proteins
luteal cells
proteins
cells
blood vessels
In Situ Hybridization

Keywords

  • Corpus luteum
  • Cow
  • Receptor
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Food Science
  • Genetics

Cite this

Localization of gene and protein expressions of tumor necrosis factor-α and tumor necrosis factor receptor types I and II in the bovine corpus luteum during the estrous cycle. / Sakumoto, R.; Vermehren, M.; Kenngott, R. A.; Okuda, K.; Sinowatz, F.

In: Journal of Animal Science, Vol. 89, No. 10, 10.2011, p. 3040-3047.

Research output: Contribution to journalArticle

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