Abstract
One of the many roles of tumor necrosis factor (TNF)-α is to control mammalian corpus luteum (CL) PG synthesis and apoptotic cell death. Here, the cellular localization of TNF-α and its type I (TNF-RI) and type II (TNF-RII) receptors in bovine luteal tissue were analyzed using in situ hybridization, immunohistochemistry, and quantitative realtime PCR. Transcripts for TNF-α were expressed in bovine CL throughout the estrous cycle, but were significantly more abundant (P <0.01) at the regressed luteal stage than at the other stages. Localization of TNF-α transcripts and protein were observed in large and small bovine luteal cells, as well as in immune cells. Moreover, transcripts for TNF-RI and TNF-RII were expressed in bovine CL throughout the estrous cycle. The abundance of TNF-RII transcripts was greater (P <0.01) at the regressed luteal stage than at the other stages, whereas TNF-RI transcript abundance did not significantly change. Expression of TNF-RI and TNFRII transcripts and proteins were observed in both the large and small luteal cells, and the proteins were also expressed in the immune cells and vascular endothelial cells. These results suggest that TNF-α sources include immune cells, as well as large and small luteal cells, and that TNF-RI and TNF-RII are present in the luteal cells of the bovine CL.
| Original language | English |
|---|---|
| Pages (from-to) | 3040-3047 |
| Number of pages | 8 |
| Journal | Journal of Animal Science |
| Volume | 89 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Oct 2011 |
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Keywords
- Corpus luteum
- Cow
- Receptor
- Tumor necrosis factor-α
ASJC Scopus subject areas
- Animal Science and Zoology
- Food Science
- Genetics
Cite this
Localization of gene and protein expressions of tumor necrosis factor-α and tumor necrosis factor receptor types I and II in the bovine corpus luteum during the estrous cycle. / Sakumoto, R.; Vermehren, M.; Kenngott, R. A.; Okuda, K.; Sinowatz, F.
In: Journal of Animal Science, Vol. 89, No. 10, 10.2011, p. 3040-3047.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Localization of gene and protein expressions of tumor necrosis factor-α and tumor necrosis factor receptor types I and II in the bovine corpus luteum during the estrous cycle
AU - Sakumoto, R.
AU - Vermehren, M.
AU - Kenngott, R. A.
AU - Okuda, K.
AU - Sinowatz, F.
PY - 2011/10
Y1 - 2011/10
N2 - One of the many roles of tumor necrosis factor (TNF)-α is to control mammalian corpus luteum (CL) PG synthesis and apoptotic cell death. Here, the cellular localization of TNF-α and its type I (TNF-RI) and type II (TNF-RII) receptors in bovine luteal tissue were analyzed using in situ hybridization, immunohistochemistry, and quantitative realtime PCR. Transcripts for TNF-α were expressed in bovine CL throughout the estrous cycle, but were significantly more abundant (P <0.01) at the regressed luteal stage than at the other stages. Localization of TNF-α transcripts and protein were observed in large and small bovine luteal cells, as well as in immune cells. Moreover, transcripts for TNF-RI and TNF-RII were expressed in bovine CL throughout the estrous cycle. The abundance of TNF-RII transcripts was greater (P <0.01) at the regressed luteal stage than at the other stages, whereas TNF-RI transcript abundance did not significantly change. Expression of TNF-RI and TNFRII transcripts and proteins were observed in both the large and small luteal cells, and the proteins were also expressed in the immune cells and vascular endothelial cells. These results suggest that TNF-α sources include immune cells, as well as large and small luteal cells, and that TNF-RI and TNF-RII are present in the luteal cells of the bovine CL.
AB - One of the many roles of tumor necrosis factor (TNF)-α is to control mammalian corpus luteum (CL) PG synthesis and apoptotic cell death. Here, the cellular localization of TNF-α and its type I (TNF-RI) and type II (TNF-RII) receptors in bovine luteal tissue were analyzed using in situ hybridization, immunohistochemistry, and quantitative realtime PCR. Transcripts for TNF-α were expressed in bovine CL throughout the estrous cycle, but were significantly more abundant (P <0.01) at the regressed luteal stage than at the other stages. Localization of TNF-α transcripts and protein were observed in large and small bovine luteal cells, as well as in immune cells. Moreover, transcripts for TNF-RI and TNF-RII were expressed in bovine CL throughout the estrous cycle. The abundance of TNF-RII transcripts was greater (P <0.01) at the regressed luteal stage than at the other stages, whereas TNF-RI transcript abundance did not significantly change. Expression of TNF-RI and TNFRII transcripts and proteins were observed in both the large and small luteal cells, and the proteins were also expressed in the immune cells and vascular endothelial cells. These results suggest that TNF-α sources include immune cells, as well as large and small luteal cells, and that TNF-RI and TNF-RII are present in the luteal cells of the bovine CL.
KW - Corpus luteum
KW - Cow
KW - Receptor
KW - Tumor necrosis factor-α
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UR - http://www.scopus.com/inward/citedby.url?scp=80052976992&partnerID=8YFLogxK
U2 - 10.2527/jas.2010-3479
DO - 10.2527/jas.2010-3479
M3 - Article
VL - 89
SP - 3040
EP - 3047
JO - Journal of Animal Science
JF - Journal of Animal Science
SN - 0021-8812
IS - 10
ER -