Localization of alpha-2 adrenergic receptors in the human eye

Toshihiko Matsuo, M. S. Cynader

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

To elucidate sites of the action of alpha-2 adrenergic drugs, we localized binding sites for a potent alpha-2 adrenergic agonist, UK-14,304, in sections of the human cadaveric eye by an in vitro ligand-binding technique and autoradiography. Its specific binding sites were found in the iris epithelium and ciliary epithelium at a high level, and also in the ciliary muscle, retina, retinal pigment epithelium (and/or choroid). Binding of UK-14,304 to the ocular pigments was prevented by preincubating the eye sections with chloroquine, which reduced the non-specific binding to a low level.

Original languageEnglish
Pages (from-to)213-219
Number of pages7
JournalOphthalmic Research
Volume24
Issue number4
Publication statusPublished - 1992

Fingerprint

Adrenergic alpha-2 Receptors
Epithelium
Adrenergic alpha-2 Receptor Agonists
Binding Sites
Choroid
Retinal Pigment Epithelium
Chloroquine
Iris
Autoradiography
Adrenergic Agents
Retina
Ligands
Muscles
Brimonidine Tartrate

Keywords

  • Alpha-2 adrenergic receptor
  • Autoradiography
  • Human eye
  • Ligand-binding study
  • UK-14,304

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Localization of alpha-2 adrenergic receptors in the human eye. / Matsuo, Toshihiko; Cynader, M. S.

In: Ophthalmic Research, Vol. 24, No. 4, 1992, p. 213-219.

Research output: Contribution to journalArticle

@article{95f742e7702e44039e34a7a779445c5f,
title = "Localization of alpha-2 adrenergic receptors in the human eye",
abstract = "To elucidate sites of the action of alpha-2 adrenergic drugs, we localized binding sites for a potent alpha-2 adrenergic agonist, UK-14,304, in sections of the human cadaveric eye by an in vitro ligand-binding technique and autoradiography. Its specific binding sites were found in the iris epithelium and ciliary epithelium at a high level, and also in the ciliary muscle, retina, retinal pigment epithelium (and/or choroid). Binding of UK-14,304 to the ocular pigments was prevented by preincubating the eye sections with chloroquine, which reduced the non-specific binding to a low level.",
keywords = "Alpha-2 adrenergic receptor, Autoradiography, Human eye, Ligand-binding study, UK-14,304",
author = "Toshihiko Matsuo and Cynader, {M. S.}",
year = "1992",
language = "English",
volume = "24",
pages = "213--219",
journal = "Ophthalmic Research",
issn = "0030-3747",
publisher = "S. Karger AG",
number = "4",

}

TY - JOUR

T1 - Localization of alpha-2 adrenergic receptors in the human eye

AU - Matsuo, Toshihiko

AU - Cynader, M. S.

PY - 1992

Y1 - 1992

N2 - To elucidate sites of the action of alpha-2 adrenergic drugs, we localized binding sites for a potent alpha-2 adrenergic agonist, UK-14,304, in sections of the human cadaveric eye by an in vitro ligand-binding technique and autoradiography. Its specific binding sites were found in the iris epithelium and ciliary epithelium at a high level, and also in the ciliary muscle, retina, retinal pigment epithelium (and/or choroid). Binding of UK-14,304 to the ocular pigments was prevented by preincubating the eye sections with chloroquine, which reduced the non-specific binding to a low level.

AB - To elucidate sites of the action of alpha-2 adrenergic drugs, we localized binding sites for a potent alpha-2 adrenergic agonist, UK-14,304, in sections of the human cadaveric eye by an in vitro ligand-binding technique and autoradiography. Its specific binding sites were found in the iris epithelium and ciliary epithelium at a high level, and also in the ciliary muscle, retina, retinal pigment epithelium (and/or choroid). Binding of UK-14,304 to the ocular pigments was prevented by preincubating the eye sections with chloroquine, which reduced the non-specific binding to a low level.

KW - Alpha-2 adrenergic receptor

KW - Autoradiography

KW - Human eye

KW - Ligand-binding study

KW - UK-14,304

UR - http://www.scopus.com/inward/record.url?scp=0026686801&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026686801&partnerID=8YFLogxK

M3 - Article

C2 - 1359487

AN - SCOPUS:0026686801

VL - 24

SP - 213

EP - 219

JO - Ophthalmic Research

JF - Ophthalmic Research

SN - 0030-3747

IS - 4

ER -