Localization of a serine proteinase inhibitor, B-43, in the bovine pancreas

Masahiro Nishibori, Naoki Nakaya, Aiji Ohtsuka, Takuro Murakami, Kiyomi Saeki

Research output: Contribution to journalArticle

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Abstract

B-43, a serine proteinase inhibitor belonging to the ovalbumin branch of the serpin superfamily, was purified and cloned from bovine brain. Since [35S]-labeled B-43 forms SDS-stable complexes with pancreatic serine proteinases, trypsin, α-chymotrypsin, and kallikrein, it has been suggested that B-43 is capable of inhibiting these serine proteinases and that B-43 may be present in the pancreas. In the present study, we investigated the localization of B-43 in the bovine pancreas immunohistochemically and examined the effect of B-43 on the amidolytic activities of pancreatic serine proteinases. Strong B-43-1ike immunoreactivity was localized in acinat cells, especially in the basal sides of the cells where the rough endoplasmic reticulum is located. The nuclei of the subpopulation of acinar cells were also immunoreactive for B-43. The recombinant glutathione S-transferase-B-43 fusion protein inhibited the amidolytic activity of trypsin and, to a lesser extent, α-chymotrypsin and kallikrein, but not elastase. These results suggest a role of B-43 in regulating serine proteinases both in the cytoplasm and the nucleus.

Original languageEnglish
Pages (from-to)51-56
Number of pages6
JournalHistochemistry and Cell Biology
Volume110
Issue number1
DOIs
Publication statusPublished - 1998

Fingerprint

yeast proteinase B
pancreas
Serine Proteinase Inhibitors
inhibitors
trypsin
Pancreas
cells
endoplasmic reticulum
Serine Proteases
nuclei
glutathione
cytoplasm
brain
fusion
Kallikreins
proteins
Brain
Fusion reactions
B 43
Peptide Hydrolases

ASJC Scopus subject areas

  • Cell Biology
  • Instrumentation

Cite this

Localization of a serine proteinase inhibitor, B-43, in the bovine pancreas. / Nishibori, Masahiro; Nakaya, Naoki; Ohtsuka, Aiji; Murakami, Takuro; Saeki, Kiyomi.

In: Histochemistry and Cell Biology, Vol. 110, No. 1, 1998, p. 51-56.

Research output: Contribution to journalArticle

Nishibori, Masahiro ; Nakaya, Naoki ; Ohtsuka, Aiji ; Murakami, Takuro ; Saeki, Kiyomi. / Localization of a serine proteinase inhibitor, B-43, in the bovine pancreas. In: Histochemistry and Cell Biology. 1998 ; Vol. 110, No. 1. pp. 51-56.
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