Localization of α1-adrenoceptor in the rat kidney: In vitro autoradiography using [3H]-bunazosin

E. Katayama, T. Ogura, K. Takatori, T. Mitsui, I. Yamamoto, Z. Ota, N. Ogawa, K. Mizukawa

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We studied the precise localization of α1-adrenoceptor in the rat kidney using in vitro macro- and micro-autoradiography (ARG) of [3H]-bunazosin. The slide-mounted nonfixed frozen sections (10 μm) of kidney were incubated with [3H]-bunazosin (5 nM) at 24°C for 45 min, and then applied to macro- and micro-ARG. The macro-ARG revealed that the bunazosin binding sites were present mainly in the rat renal cortex. These bindings were evaluated to be specific by means of computerized image analysis system (RAS 1,000) of ARG. Micro-autoradiogram showed that the tubules in the renal cortex had higher specific grain density than the glomeruli. These findings suggested that α1-adrenoceptor in the rat kidney located mainly on the cortical tubules.

Original languageEnglish
Pages (from-to)205-213
Number of pages9
JournalResearch Communications in Chemical Pathology and Pharmacology
Volume64
Issue number2
Publication statusPublished - 1989

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Autoradiography
Adrenergic Receptors
Rats
Kidney
Macros
Frozen Sections
Image analysis
Binding Sites
bunazosin
In Vitro Techniques

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Katayama, E., Ogura, T., Takatori, K., Mitsui, T., Yamamoto, I., Ota, Z., ... Mizukawa, K. (1989). Localization of α1-adrenoceptor in the rat kidney: In vitro autoradiography using [3H]-bunazosin. Research Communications in Chemical Pathology and Pharmacology, 64(2), 205-213.

Localization of α1-adrenoceptor in the rat kidney : In vitro autoradiography using [3H]-bunazosin. / Katayama, E.; Ogura, T.; Takatori, K.; Mitsui, T.; Yamamoto, I.; Ota, Z.; Ogawa, N.; Mizukawa, K.

In: Research Communications in Chemical Pathology and Pharmacology, Vol. 64, No. 2, 1989, p. 205-213.

Research output: Contribution to journalArticle

Katayama, E, Ogura, T, Takatori, K, Mitsui, T, Yamamoto, I, Ota, Z, Ogawa, N & Mizukawa, K 1989, 'Localization of α1-adrenoceptor in the rat kidney: In vitro autoradiography using [3H]-bunazosin', Research Communications in Chemical Pathology and Pharmacology, vol. 64, no. 2, pp. 205-213.
Katayama, E. ; Ogura, T. ; Takatori, K. ; Mitsui, T. ; Yamamoto, I. ; Ota, Z. ; Ogawa, N. ; Mizukawa, K. / Localization of α1-adrenoceptor in the rat kidney : In vitro autoradiography using [3H]-bunazosin. In: Research Communications in Chemical Pathology and Pharmacology. 1989 ; Vol. 64, No. 2. pp. 205-213.
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