Localization and responsiveness of a cowpea apyrase VsNTPase1 to phytopathogenic microorganisms

Hirotaka Takahashi, Kazuhiro Toyoda, Yuzo Hirakawa, Kunihiko Morishita, Toshiaki Kato, Yoshishige Inagaki, Yuki Ichinose, Tomonori Shiraishi

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Apyrases (NTPases) are associated with both compatible and incompatible interactions between plants and microorganisms. Previously we reported that the ATPase activities of cell-wall-bound apyrases of several leguminous plants, such as pea, cowpea, soybean, and kidney bean, were enhanced by a glycoprotein elicitor and were inhibited in a species-specific manner by mucin-type glycopeptide suppressors secreted from a pea pathogenic fungus, Mycosphaerella pinodes. In this study, we isolated two apyrase genes, VsNTPase1 and VsNTPase2, from a cDNA library of Vigna sinensis Endl. cv. Sanjakusasage. Based on phylogenetic analysis, VsNTPase1 may belong to a group that responds to environmental stimuli. In a transient assay using DNA bombardment, a fusion protein of green fluorescent protein (GFP) and the N-terminal putative signal sequence of VsNTPase1 was distributed in the nucleus, cytoplasm (cytoskeletal structure), and cell wall. On the other hand, a fusion protein of GFP and the N-terminal putative VsNTPase2-signal sequence was localized in the cytoplasm, especially in small particles (perhaps mitochondria). A recombinant VsNTPase1 expressed in Spodoptera frugiperda 21 cells responded directly to signal molecules from several phytopathogenic microorganisms. Here, we discuss the role of apyrases in recognizing and responding to exogenous signals.

Original languageEnglish
Pages (from-to)143-151
Number of pages9
JournalJournal of General Plant Pathology
Volume72
Issue number3
DOIs
Publication statusPublished - Jun 1 2006

Keywords

  • Apyrase
  • Elicitor
  • Mycosphaerella pinodes
  • Suppressor
  • Vigna sinensis Endl., cv. Sanjakusasage

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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