Localization and expression of serine racemase in Arabidopsis thaliana

Manabu Sugimoto; Wataru Sakamoto; Yoshiyuki Fujitani

doi:10.1007/s00726-008-0112-z

Localization and expression of serine racemase in Arabidopsis thaliana

Manabu Sugimoto, Wataru Sakamoto, Yoshiyuki Fujitani

Institute of Plant Science and Resources

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Arabidopsis plants transformed by promoter of A. thaliana serine racemase fused with β-glucuronidase (GUS) reporter gene showed strong GUS staining in elongating and developing cells such as tip regions of primary and lateral roots, developing leaves, and shoot meristems. RT-PCR and digital northern hybridization showed that expression of the serine racemase gene was not induced by l- and d-serine, light irradiation, biotic and abiotic stresses.

Original language	English
Pages (from-to)	587-590
Number of pages	4
Journal	Amino Acids
Volume	36
Issue number	3
DOIs	https://doi.org/10.1007/s00726-008-0112-z
Publication status	Published - Mar 2009

Keywords

Arabidopsis thaliana
D-amino acid
GUS
Promoter
Serine racemase

ASJC Scopus subject areas

Biochemistry
Clinical Biochemistry
Organic Chemistry

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10.1007/s00726-008-0112-z

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abstract = "Arabidopsis plants transformed by promoter of A. thaliana serine racemase fused with β-glucuronidase (GUS) reporter gene showed strong GUS staining in elongating and developing cells such as tip regions of primary and lateral roots, developing leaves, and shoot meristems. RT-PCR and digital northern hybridization showed that expression of the serine racemase gene was not induced by l- and d-serine, light irradiation, biotic and abiotic stresses.",

keywords = "Arabidopsis thaliana, D-amino acid, GUS, Promoter, Serine racemase",

author = "Manabu Sugimoto and Wataru Sakamoto and Yoshiyuki Fujitani",

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AU - Sugimoto, Manabu

AU - Sakamoto, Wataru

AU - Fujitani, Yoshiyuki

N1 - Funding Information: This research was supported in part by the Ohara Foundation in Kurashiki, Japan.

PY - 2009/3

Y1 - 2009/3

N2 - Arabidopsis plants transformed by promoter of A. thaliana serine racemase fused with β-glucuronidase (GUS) reporter gene showed strong GUS staining in elongating and developing cells such as tip regions of primary and lateral roots, developing leaves, and shoot meristems. RT-PCR and digital northern hybridization showed that expression of the serine racemase gene was not induced by l- and d-serine, light irradiation, biotic and abiotic stresses.

AB - Arabidopsis plants transformed by promoter of A. thaliana serine racemase fused with β-glucuronidase (GUS) reporter gene showed strong GUS staining in elongating and developing cells such as tip regions of primary and lateral roots, developing leaves, and shoot meristems. RT-PCR and digital northern hybridization showed that expression of the serine racemase gene was not induced by l- and d-serine, light irradiation, biotic and abiotic stresses.

KW - Arabidopsis thaliana

KW - D-amino acid

KW - GUS

KW - Promoter

KW - Serine racemase

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Localization and expression of serine racemase in Arabidopsis thaliana

Abstract

Keywords

ASJC Scopus subject areas

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