Abstract
Arabidopsis plants transformed by promoter of A. thaliana serine racemase fused with β-glucuronidase (GUS) reporter gene showed strong GUS staining in elongating and developing cells such as tip regions of primary and lateral roots, developing leaves, and shoot meristems. RT-PCR and digital northern hybridization showed that expression of the serine racemase gene was not induced by l- and d-serine, light irradiation, biotic and abiotic stresses.
| Original language | English |
|---|---|
| Pages (from-to) | 587-590 |
| Number of pages | 4 |
| Journal | Amino Acids |
| Volume | 36 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 2009 |
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Keywords
- Arabidopsis thaliana
- D-amino acid
- GUS
- Promoter
- Serine racemase
ASJC Scopus subject areas
- Biochemistry
- Clinical Biochemistry
- Organic Chemistry
Cite this
Localization and expression of serine racemase in Arabidopsis thaliana. / Sugimoto, Manabu; Sakamoto, Wataru; Fujitani, Yoshiyuki.
In: Amino Acids, Vol. 36, No. 3, 03.2009, p. 587-590.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Localization and expression of serine racemase in Arabidopsis thaliana
AU - Sugimoto, Manabu
AU - Sakamoto, Wataru
AU - Fujitani, Yoshiyuki
PY - 2009/3
Y1 - 2009/3
N2 - Arabidopsis plants transformed by promoter of A. thaliana serine racemase fused with β-glucuronidase (GUS) reporter gene showed strong GUS staining in elongating and developing cells such as tip regions of primary and lateral roots, developing leaves, and shoot meristems. RT-PCR and digital northern hybridization showed that expression of the serine racemase gene was not induced by l- and d-serine, light irradiation, biotic and abiotic stresses.
AB - Arabidopsis plants transformed by promoter of A. thaliana serine racemase fused with β-glucuronidase (GUS) reporter gene showed strong GUS staining in elongating and developing cells such as tip regions of primary and lateral roots, developing leaves, and shoot meristems. RT-PCR and digital northern hybridization showed that expression of the serine racemase gene was not induced by l- and d-serine, light irradiation, biotic and abiotic stresses.
KW - Arabidopsis thaliana
KW - D-amino acid
KW - GUS
KW - Promoter
KW - Serine racemase
UR - http://www.scopus.com/inward/record.url?scp=67349099097&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67349099097&partnerID=8YFLogxK
U2 - 10.1007/s00726-008-0112-z
DO - 10.1007/s00726-008-0112-z
M3 - Article
C2 - 18546053
AN - SCOPUS:67349099097
VL - 36
SP - 587
EP - 590
JO - Amino Acids
JF - Amino Acids
SN - 0939-4451
IS - 3
ER -