Local effects of intra-articular injection of anti-rabbit tumor necrosis factor alpha monoclonal antibody in antigen-induced arthritis of the rabbit temporomandibular joint

Taishi Ohtani, Manabu Habu, Amit Khanal, Izumi Yoshioka, Akihiro Matsukawa, Kazuhiro Tominaga

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Local effects of intra-articular (IA) injection of anti-rabbit tumor necrosis factor alpha monoclonal antibody (anti-TNFα mAb) for antigen-induced mono-arthritis (AIA) of the rabbit temporomandibular joint (TMJ) and its systemic influences were evaluated. Methods: Biochemical analysis of synovial fluid (SF), clinical and histopathological analyses of TMJ, and serum analysis were performed after inducing AIA in bilateral TMJs of 40 New Zealand White rabbits. IA injection of anti-TNFα mAb in unilateral TMJ (TNF blockade side; n=12) and IgG (n=12) was performed while saline injected on the contralateral side. TNFα and IL-1β in SF was analyzed at Days 1, 3, 7, and 21. Joint swelling and head withdrawal reflex threshold (WRT) over TMJs were evaluated in TNF blockade side (n=12) with histopathological analysis at Days 3, 7, and 21. In remaining four animals with TNF blockade, TNFα and anti-TNFα mAb in serum were analyzed. Results: Tumor necrosis factor alpha in SF was significantly lower in TNF blockade side on all days but IL-1β was lower only on Day 3. WRT was significantly higher at all times in the blockade side. Less inflammatory reactions and degenerative changes of cartilage were observed on Days 7 and 21 in the blockade side. TNFα and anti-TNFα mAb were under detection level in serum at all times. Conclusions: Intra-articular injection of anti-rabbit TNFα mAb in mono-arthritis model was effective to control local inflammation and degenerative joint changes. Further, low-dose IA injection of antibody may not have systemic side effects.

Original languageEnglish
Pages (from-to)96-105
Number of pages10
JournalJournal of Oral Pathology and Medicine
Volume41
Issue number1
DOIs
Publication statusPublished - Jan 2012

Fingerprint

Intra-Articular Injections
Temporomandibular Joint
Arthritis
Tumor Necrosis Factor-alpha
Monoclonal Antibodies
Rabbits
Antigens
Synovial Fluid
Interleukin-1
Reflex
Joints
Serum
Cartilage
Immunoglobulin G
Head
Inflammation
Antibodies

Keywords

  • Antigen-induced arthritis
  • Intra-articular injection
  • Rabbit
  • Temporomandibular joint
  • Tumor necrosis factor alpha

ASJC Scopus subject areas

  • Cancer Research
  • Pathology and Forensic Medicine
  • Otorhinolaryngology
  • Oral Surgery
  • Periodontics

Cite this

Local effects of intra-articular injection of anti-rabbit tumor necrosis factor alpha monoclonal antibody in antigen-induced arthritis of the rabbit temporomandibular joint. / Ohtani, Taishi; Habu, Manabu; Khanal, Amit; Yoshioka, Izumi; Matsukawa, Akihiro; Tominaga, Kazuhiro.

In: Journal of Oral Pathology and Medicine, Vol. 41, No. 1, 01.2012, p. 96-105.

Research output: Contribution to journalArticle

@article{8d9fd0a693ab4f2fbdcafee9267a9dec,
title = "Local effects of intra-articular injection of anti-rabbit tumor necrosis factor alpha monoclonal antibody in antigen-induced arthritis of the rabbit temporomandibular joint",
abstract = "Background: Local effects of intra-articular (IA) injection of anti-rabbit tumor necrosis factor alpha monoclonal antibody (anti-TNFα mAb) for antigen-induced mono-arthritis (AIA) of the rabbit temporomandibular joint (TMJ) and its systemic influences were evaluated. Methods: Biochemical analysis of synovial fluid (SF), clinical and histopathological analyses of TMJ, and serum analysis were performed after inducing AIA in bilateral TMJs of 40 New Zealand White rabbits. IA injection of anti-TNFα mAb in unilateral TMJ (TNF blockade side; n=12) and IgG (n=12) was performed while saline injected on the contralateral side. TNFα and IL-1β in SF was analyzed at Days 1, 3, 7, and 21. Joint swelling and head withdrawal reflex threshold (WRT) over TMJs were evaluated in TNF blockade side (n=12) with histopathological analysis at Days 3, 7, and 21. In remaining four animals with TNF blockade, TNFα and anti-TNFα mAb in serum were analyzed. Results: Tumor necrosis factor alpha in SF was significantly lower in TNF blockade side on all days but IL-1β was lower only on Day 3. WRT was significantly higher at all times in the blockade side. Less inflammatory reactions and degenerative changes of cartilage were observed on Days 7 and 21 in the blockade side. TNFα and anti-TNFα mAb were under detection level in serum at all times. Conclusions: Intra-articular injection of anti-rabbit TNFα mAb in mono-arthritis model was effective to control local inflammation and degenerative joint changes. Further, low-dose IA injection of antibody may not have systemic side effects.",
keywords = "Antigen-induced arthritis, Intra-articular injection, Rabbit, Temporomandibular joint, Tumor necrosis factor alpha",
author = "Taishi Ohtani and Manabu Habu and Amit Khanal and Izumi Yoshioka and Akihiro Matsukawa and Kazuhiro Tominaga",
year = "2012",
month = "1",
doi = "10.1111/j.1600-0714.2011.01056.x",
language = "English",
volume = "41",
pages = "96--105",
journal = "Journal of Oral Pathology and Medicine",
issn = "0904-2512",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Local effects of intra-articular injection of anti-rabbit tumor necrosis factor alpha monoclonal antibody in antigen-induced arthritis of the rabbit temporomandibular joint

AU - Ohtani, Taishi

AU - Habu, Manabu

AU - Khanal, Amit

AU - Yoshioka, Izumi

AU - Matsukawa, Akihiro

AU - Tominaga, Kazuhiro

PY - 2012/1

Y1 - 2012/1

N2 - Background: Local effects of intra-articular (IA) injection of anti-rabbit tumor necrosis factor alpha monoclonal antibody (anti-TNFα mAb) for antigen-induced mono-arthritis (AIA) of the rabbit temporomandibular joint (TMJ) and its systemic influences were evaluated. Methods: Biochemical analysis of synovial fluid (SF), clinical and histopathological analyses of TMJ, and serum analysis were performed after inducing AIA in bilateral TMJs of 40 New Zealand White rabbits. IA injection of anti-TNFα mAb in unilateral TMJ (TNF blockade side; n=12) and IgG (n=12) was performed while saline injected on the contralateral side. TNFα and IL-1β in SF was analyzed at Days 1, 3, 7, and 21. Joint swelling and head withdrawal reflex threshold (WRT) over TMJs were evaluated in TNF blockade side (n=12) with histopathological analysis at Days 3, 7, and 21. In remaining four animals with TNF blockade, TNFα and anti-TNFα mAb in serum were analyzed. Results: Tumor necrosis factor alpha in SF was significantly lower in TNF blockade side on all days but IL-1β was lower only on Day 3. WRT was significantly higher at all times in the blockade side. Less inflammatory reactions and degenerative changes of cartilage were observed on Days 7 and 21 in the blockade side. TNFα and anti-TNFα mAb were under detection level in serum at all times. Conclusions: Intra-articular injection of anti-rabbit TNFα mAb in mono-arthritis model was effective to control local inflammation and degenerative joint changes. Further, low-dose IA injection of antibody may not have systemic side effects.

AB - Background: Local effects of intra-articular (IA) injection of anti-rabbit tumor necrosis factor alpha monoclonal antibody (anti-TNFα mAb) for antigen-induced mono-arthritis (AIA) of the rabbit temporomandibular joint (TMJ) and its systemic influences were evaluated. Methods: Biochemical analysis of synovial fluid (SF), clinical and histopathological analyses of TMJ, and serum analysis were performed after inducing AIA in bilateral TMJs of 40 New Zealand White rabbits. IA injection of anti-TNFα mAb in unilateral TMJ (TNF blockade side; n=12) and IgG (n=12) was performed while saline injected on the contralateral side. TNFα and IL-1β in SF was analyzed at Days 1, 3, 7, and 21. Joint swelling and head withdrawal reflex threshold (WRT) over TMJs were evaluated in TNF blockade side (n=12) with histopathological analysis at Days 3, 7, and 21. In remaining four animals with TNF blockade, TNFα and anti-TNFα mAb in serum were analyzed. Results: Tumor necrosis factor alpha in SF was significantly lower in TNF blockade side on all days but IL-1β was lower only on Day 3. WRT was significantly higher at all times in the blockade side. Less inflammatory reactions and degenerative changes of cartilage were observed on Days 7 and 21 in the blockade side. TNFα and anti-TNFα mAb were under detection level in serum at all times. Conclusions: Intra-articular injection of anti-rabbit TNFα mAb in mono-arthritis model was effective to control local inflammation and degenerative joint changes. Further, low-dose IA injection of antibody may not have systemic side effects.

KW - Antigen-induced arthritis

KW - Intra-articular injection

KW - Rabbit

KW - Temporomandibular joint

KW - Tumor necrosis factor alpha

UR - http://www.scopus.com/inward/record.url?scp=84855453062&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84855453062&partnerID=8YFLogxK

U2 - 10.1111/j.1600-0714.2011.01056.x

DO - 10.1111/j.1600-0714.2011.01056.x

M3 - Article

C2 - 22004078

AN - SCOPUS:84855453062

VL - 41

SP - 96

EP - 105

JO - Journal of Oral Pathology and Medicine

JF - Journal of Oral Pathology and Medicine

SN - 0904-2512

IS - 1

ER -