Local effect of lysophosphatidic acid on prostaglandin production in the bovine oviduct

Yuka Yoshimoto, Yoshihiko Kobayashi, Izabela Woclawek-Potocka, Emilia Sinderewicz, Yuki Yamamoto, Koji Kimura, Kiyoshi Okuda

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The mammalian oviduct plays an important role in the fertilisation and transport of gametes and embryo. Prostaglandins (PGs) are local mediators of oviductal functions and are involved in fertilisation and the transport of gametes and embryo. Lysophosphatidic acid (LPA), a kind of phospholipid, is involved in various physiological actions. We hypothesised that LPA regulates PG production in the bovine oviduct. To test this hypothesis, we examined the mRNA expression of LPA receptors (LPAR1-6) and LPA-producing enzymes (ATX, PLA1α, PLA1β) in ampullary and isthmic tissues and in cultured epithelial and stromal cells isolated from the bovine oviduct. We also investigated the effects of LPA on PG synthase expression and PG production in cultured cells. The mRNA of LPAR1-4, 6, ATX and PLA1α were expressed in cultured epithelial and stromal cells. The expressions of LPAR1-3 were significantly lower and the expression of LPAR4 was significantly higher in the isthmic than in the ampullary tissues. Lysophosphatidic acid significantly stimulated PG production in the cultured isthmic stromal cells. The overall findings suggest that LPA stimulates PG production via LPAR4 in the bovine oviduct. Since PGs are important for fertilisation and the transport of gametes and embryo, these findings show that locally produced LPA regulates oviductal functions.

Original languageEnglish
Pages (from-to)1021-1028
Number of pages8
JournalReproduction, Fertility and Development
Volume29
Issue number5
DOIs
Publication statusPublished - 2017

Fingerprint

Oviducts
oviducts
prostaglandins
Prostaglandins
acids
cattle
cultured cells
Stromal Cells
stromal cells
Fertilization
Germ Cells
Cultured Cells
Embryonic Structures
germ cells
embryo (animal)
Epithelial Cells
Lysophosphatidic Acid Receptors
epithelial cells
Messenger RNA
fallopian tubes

Keywords

  • cultured cells
  • cyclooxygenase
  • smooth muscle movement
  • stromal cells

ASJC Scopus subject areas

  • Biotechnology
  • Reproductive Medicine
  • Molecular Biology
  • Animal Science and Zoology
  • Genetics
  • Endocrinology
  • Developmental Biology

Cite this

Local effect of lysophosphatidic acid on prostaglandin production in the bovine oviduct. / Yoshimoto, Yuka; Kobayashi, Yoshihiko; Woclawek-Potocka, Izabela; Sinderewicz, Emilia; Yamamoto, Yuki; Kimura, Koji; Okuda, Kiyoshi.

In: Reproduction, Fertility and Development, Vol. 29, No. 5, 2017, p. 1021-1028.

Research output: Contribution to journalArticle

Yoshimoto, Yuka ; Kobayashi, Yoshihiko ; Woclawek-Potocka, Izabela ; Sinderewicz, Emilia ; Yamamoto, Yuki ; Kimura, Koji ; Okuda, Kiyoshi. / Local effect of lysophosphatidic acid on prostaglandin production in the bovine oviduct. In: Reproduction, Fertility and Development. 2017 ; Vol. 29, No. 5. pp. 1021-1028.
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