Local and Systemic Immune Responses to Influenza A Virus Infection in Pneumonia and Encephalitis Mouse Models

Yoshiharu Nagaoka, Nobuyuki Nosaka, Mutsuko Yamada, Masato Yashiro, Yousuke Washio, Kenji Baba, Tsuneo Morishima, Hirokazu Tsukahara

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective. To compare local and systemic profiles between different disease pathologies (pneumonia and encephalitis) induced by influenza A virus (IAV). Methods. An IAV pneumonia model was created by intranasal inoculation of C57BL/6 mice with influenza A/WSN/33 (H1N1) virus. Lung lavage and blood collection were performed on day 3 after IAV inoculation. Similarly, an IAV encephalitis mouse model was created by direct intracranial IAV inoculation. Cerebrospinal fluid (CSF) and blood collection were conducted according to the same schedule. Cytokine/chemokine profiles were produced for each collected sample. Then the data were compared visually using radar charts. Results. Serum cytokine profiles were similar in pneumonia and encephalitis models, but local responses between the bronchoalveolar lavage fluid (BALF) in the pneumonia model and CSF in the encephalitis model differed. Moreover, to varying degrees, the profiles of local cytokines/chemokines differed from those of serum in both the pneumonia and encephalitis models. Conclusion. Investigating local samples such as BALF and CSF is important for evaluating local immune responses, providing insight into pathology at the primary loci of infection. Serum data alone might be insufficient to elucidate local immune responses and might not enable clinicians to devise the most appropriate treatment strategies.

Original languageEnglish
Article number2594231
JournalDisease Markers
Volume2017
DOIs
Publication statusPublished - 2017

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Influenza A virus
Virus Diseases
Encephalitis
Viruses
Pneumonia
Cerebrospinal fluid
Cerebrospinal Fluid
Bronchoalveolar Lavage Fluid
Cytokines
Chemokines
Pathology
Serum
Radar
Blood
H1N1 Subtype Influenza A Virus
Bronchoalveolar Lavage
Inbred C57BL Mouse
Fluids
Human Influenza
Appointments and Schedules

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Local and Systemic Immune Responses to Influenza A Virus Infection in Pneumonia and Encephalitis Mouse Models. / Nagaoka, Yoshiharu; Nosaka, Nobuyuki; Yamada, Mutsuko; Yashiro, Masato; Washio, Yousuke; Baba, Kenji; Morishima, Tsuneo; Tsukahara, Hirokazu.

In: Disease Markers, Vol. 2017, 2594231, 2017.

Research output: Contribution to journalArticle

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AU - Nagaoka, Yoshiharu

AU - Nosaka, Nobuyuki

AU - Yamada, Mutsuko

AU - Yashiro, Masato

AU - Washio, Yousuke

AU - Baba, Kenji

AU - Morishima, Tsuneo

AU - Tsukahara, Hirokazu

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