Load-dependency in mechanical properties of subepicardial and subendocardial cardiomyocytes

Anastasia Khokhlova, Gentaro Iribe, Olga Solovyova

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Electrical and mechanical properties of myocardium vary transmurally in the left ventricular wall. Regional differences in the mechanical environment of cardiomyocytes may potentially contribute to this heterogeneity due to mechano-electric feedback. In the present study, we investigate transmural differences in active and passive tensions at different preloads between the subepicardial (EPI) and subendocardial (ENDO) cells isolated from mouse left ventricle, using our recently developed single cell stretch method where each cell end was held by a pair of carbon fibers to apply various extent of preload to the cells. To predict underlying mechanisms of the transmural differences, we used our electromechanical EPI and ENDO cell models to simulate experimentally obtained results. Wet experiments showed that both passive and active tensions at different preloads are higher in ENDO cardiomyocytes, indicating higher stiffness and contractility in ENDO cells compared to EPI cells. Our mathematical models reproduce experimental results, suggesting differences in the kinetics of cross bridges and calcium-troponin C complexes in ENDO and EPI models may essentially contribute to the differences in mechanical properties between the cells.

Original languageEnglish
Title of host publicationComputing in Cardiology Conference 2015, CinC 2015
EditorsAlan Murray
PublisherIEEE Computer Society
Pages965-968
Number of pages4
ISBN (Electronic)9781509006854
DOIs
Publication statusPublished - Feb 16 2015
Event42nd Computing in Cardiology Conference, CinC 2015 - Nice, France
Duration: Sep 6 2015Sep 9 2015

Publication series

NameComputing in Cardiology
Volume42
ISSN (Print)2325-8861
ISSN (Electronic)2325-887X

Other

Other42nd Computing in Cardiology Conference, CinC 2015
CountryFrance
CityNice
Period9/6/159/9/15

ASJC Scopus subject areas

  • Computer Science(all)
  • Cardiology and Cardiovascular Medicine

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  • Cite this

    Khokhlova, A., Iribe, G., & Solovyova, O. (2015). Load-dependency in mechanical properties of subepicardial and subendocardial cardiomyocytes. In A. Murray (Ed.), Computing in Cardiology Conference 2015, CinC 2015 (pp. 965-968). [7411073] (Computing in Cardiology; Vol. 42). IEEE Computer Society. https://doi.org/10.1109/CIC.2015.7411073