LjMATE1: A citrate transporter responsible for iron supply to the nodule infection zone of lotus japonicus

Kojiro Takanashi, Kengo Yokosho, Kazuhiko Saeki, Akifumi Sugiyama, Shusei Sato, Satoshi Tabata, Jian Feng Ma, Kazufumi Yazaki

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Symbiotic nitrogen fixation by intracellular rhizobia within legume root nodules requires the exchange of nutrients between host plant cells and their resident bacteria. While exchanged molecules imply nitrogen compounds, carbohydrates and also various minerals, knowledge of the molecular basis of plant transporters that mediate those metabolite exchanges is still limited. In this study, we have shown that a multidrug and toxic compound extrusion (MATE) protein, LjMATE1, is specifically induced during nodule formation, which nearly paralleled nodule maturation, in a model legume Lotus japonicus. Reporter gene experiments indicated that the expression of LjMATE1 was restricted to the infection zone of nodules. To characterize the transport function of LjMATE1, we conducted a biochemical analysis using a heterologous expression system, Xenopus oocytes, and found that LjMATE1 is a specific transporter for citrate. The physiological role of LjMATE1 was analyzed after generation of L. japonicus RNA interference (RNAi) lines. One RNAi knock-down line revealed limited growth under nitrogen-deficient conditions with inoculation of rhizobia compared with the controls (the wild type and an RNAi line in which LjMATE1 was not suppressed). It was noteworthy that Fe localization was clearly altered in nodule tissues of the knock-down line. These results strongly suggest that LjMATE1 is a nodule-specific transporter that assists the translocation of Fe from the root to nodules by providing citrate.

Original languageEnglish
Pages (from-to)585-594
Number of pages10
JournalPlant and Cell Physiology
Volume54
Issue number4
DOIs
Publication statusPublished - Apr 2013

Fingerprint

Lotus corniculatus var. japonicus
RNA Interference
RNA interference
citrates
transporters
Rhizobium
Iron
iron
Fabaceae
legumes
Infection
infection
Nitrogen Compounds
Nitrogen Fixation
Poisons
nitrogen compounds
Plant Cells
Xenopus
root nodules
toxic substances

Keywords

  • Citrate
  • Fe translocation
  • Lotus japonicus
  • MATE
  • Nodulation

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

LjMATE1 : A citrate transporter responsible for iron supply to the nodule infection zone of lotus japonicus. / Takanashi, Kojiro; Yokosho, Kengo; Saeki, Kazuhiko; Sugiyama, Akifumi; Sato, Shusei; Tabata, Satoshi; Ma, Jian Feng; Yazaki, Kazufumi.

In: Plant and Cell Physiology, Vol. 54, No. 4, 04.2013, p. 585-594.

Research output: Contribution to journalArticle

Takanashi, Kojiro ; Yokosho, Kengo ; Saeki, Kazuhiko ; Sugiyama, Akifumi ; Sato, Shusei ; Tabata, Satoshi ; Ma, Jian Feng ; Yazaki, Kazufumi. / LjMATE1 : A citrate transporter responsible for iron supply to the nodule infection zone of lotus japonicus. In: Plant and Cell Physiology. 2013 ; Vol. 54, No. 4. pp. 585-594.
@article{6a093d01d83b494bb51934e379237695,
title = "LjMATE1: A citrate transporter responsible for iron supply to the nodule infection zone of lotus japonicus",
abstract = "Symbiotic nitrogen fixation by intracellular rhizobia within legume root nodules requires the exchange of nutrients between host plant cells and their resident bacteria. While exchanged molecules imply nitrogen compounds, carbohydrates and also various minerals, knowledge of the molecular basis of plant transporters that mediate those metabolite exchanges is still limited. In this study, we have shown that a multidrug and toxic compound extrusion (MATE) protein, LjMATE1, is specifically induced during nodule formation, which nearly paralleled nodule maturation, in a model legume Lotus japonicus. Reporter gene experiments indicated that the expression of LjMATE1 was restricted to the infection zone of nodules. To characterize the transport function of LjMATE1, we conducted a biochemical analysis using a heterologous expression system, Xenopus oocytes, and found that LjMATE1 is a specific transporter for citrate. The physiological role of LjMATE1 was analyzed after generation of L. japonicus RNA interference (RNAi) lines. One RNAi knock-down line revealed limited growth under nitrogen-deficient conditions with inoculation of rhizobia compared with the controls (the wild type and an RNAi line in which LjMATE1 was not suppressed). It was noteworthy that Fe localization was clearly altered in nodule tissues of the knock-down line. These results strongly suggest that LjMATE1 is a nodule-specific transporter that assists the translocation of Fe from the root to nodules by providing citrate.",
keywords = "Citrate, Fe translocation, Lotus japonicus, MATE, Nodulation",
author = "Kojiro Takanashi and Kengo Yokosho and Kazuhiko Saeki and Akifumi Sugiyama and Shusei Sato and Satoshi Tabata and Ma, {Jian Feng} and Kazufumi Yazaki",
year = "2013",
month = "4",
doi = "10.1093/pcp/pct019",
language = "English",
volume = "54",
pages = "585--594",
journal = "Plant and Cell Physiology",
issn = "0032-0781",
publisher = "Oxford University Press",
number = "4",

}

TY - JOUR

T1 - LjMATE1

T2 - A citrate transporter responsible for iron supply to the nodule infection zone of lotus japonicus

AU - Takanashi, Kojiro

AU - Yokosho, Kengo

AU - Saeki, Kazuhiko

AU - Sugiyama, Akifumi

AU - Sato, Shusei

AU - Tabata, Satoshi

AU - Ma, Jian Feng

AU - Yazaki, Kazufumi

PY - 2013/4

Y1 - 2013/4

N2 - Symbiotic nitrogen fixation by intracellular rhizobia within legume root nodules requires the exchange of nutrients between host plant cells and their resident bacteria. While exchanged molecules imply nitrogen compounds, carbohydrates and also various minerals, knowledge of the molecular basis of plant transporters that mediate those metabolite exchanges is still limited. In this study, we have shown that a multidrug and toxic compound extrusion (MATE) protein, LjMATE1, is specifically induced during nodule formation, which nearly paralleled nodule maturation, in a model legume Lotus japonicus. Reporter gene experiments indicated that the expression of LjMATE1 was restricted to the infection zone of nodules. To characterize the transport function of LjMATE1, we conducted a biochemical analysis using a heterologous expression system, Xenopus oocytes, and found that LjMATE1 is a specific transporter for citrate. The physiological role of LjMATE1 was analyzed after generation of L. japonicus RNA interference (RNAi) lines. One RNAi knock-down line revealed limited growth under nitrogen-deficient conditions with inoculation of rhizobia compared with the controls (the wild type and an RNAi line in which LjMATE1 was not suppressed). It was noteworthy that Fe localization was clearly altered in nodule tissues of the knock-down line. These results strongly suggest that LjMATE1 is a nodule-specific transporter that assists the translocation of Fe from the root to nodules by providing citrate.

AB - Symbiotic nitrogen fixation by intracellular rhizobia within legume root nodules requires the exchange of nutrients between host plant cells and their resident bacteria. While exchanged molecules imply nitrogen compounds, carbohydrates and also various minerals, knowledge of the molecular basis of plant transporters that mediate those metabolite exchanges is still limited. In this study, we have shown that a multidrug and toxic compound extrusion (MATE) protein, LjMATE1, is specifically induced during nodule formation, which nearly paralleled nodule maturation, in a model legume Lotus japonicus. Reporter gene experiments indicated that the expression of LjMATE1 was restricted to the infection zone of nodules. To characterize the transport function of LjMATE1, we conducted a biochemical analysis using a heterologous expression system, Xenopus oocytes, and found that LjMATE1 is a specific transporter for citrate. The physiological role of LjMATE1 was analyzed after generation of L. japonicus RNA interference (RNAi) lines. One RNAi knock-down line revealed limited growth under nitrogen-deficient conditions with inoculation of rhizobia compared with the controls (the wild type and an RNAi line in which LjMATE1 was not suppressed). It was noteworthy that Fe localization was clearly altered in nodule tissues of the knock-down line. These results strongly suggest that LjMATE1 is a nodule-specific transporter that assists the translocation of Fe from the root to nodules by providing citrate.

KW - Citrate

KW - Fe translocation

KW - Lotus japonicus

KW - MATE

KW - Nodulation

UR - http://www.scopus.com/inward/record.url?scp=84875970123&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875970123&partnerID=8YFLogxK

U2 - 10.1093/pcp/pct019

DO - 10.1093/pcp/pct019

M3 - Article

C2 - 23385147

AN - SCOPUS:84875970123

VL - 54

SP - 585

EP - 594

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 4

ER -