Liver damage induced by bile duct ligation affects CYP isoenzymes differently in rats

Tomonori Tateishi, Minoru Watanabe, Hironori Nakura, Masami Tanaka, Toshio Kumai, Shinichi Kobayashi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We examined the influence of liver damage induced by bile duct ligation on the activity and the expression of hepatic cytochrome P450 (CYP) 1A, 2B, 2C6, 2C11, 2E1 and 3A2 in male Sprague-Dawley rats. In the ligation group, testosterone 2α-, 16α-, and 6β-hydroxylase activities were severely decreased, whereas ethoxyresorufin O-deethylase and progesterone 21-hydroxylase activities relatively remained. Pentoxyresorufin O-deethylase and chlorzoxazone 6-hydroxylase activities were reduced to approximately one thirds those of control. The protein contents of these isoenzymes expressed in hepatic microsomes of the ligation group were decreased to 45%, 32%, 79%, 13%, 58%, and 23% of control for CYP1A, 2B, 2C6, 2C11, 2E1 and 3A2, respectively. The rank order of magnitude of the influence of bile duct ligation on CYP isoenzymes, assessed by the reduction in the enzyme activity and the protein content, corresponded with each other except CYP1A. The reduction of the enzyme activities significantly correlated with the reduction in the protein contents of different isoenzymes. These results suggested that bile duct ligation affected CYP isoenzyme activities and contents with different extent.

Original languageEnglish
Pages (from-to)89-92
Number of pages4
JournalPharmacology and Toxicology
Volume82
Issue number2
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Bile Ducts
Liver
Ducts
Cytochrome P-450 Enzyme System
Isoenzymes
Ligation
Rats
Enzyme activity
Mixed Function Oxygenases
Chlorzoxazone
Steroid 21-Hydroxylase
Cytochrome P-450 CYP1A1
Proteins
Testosterone
Enzymes
Microsomes
Sprague Dawley Rats

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Tateishi, T., Watanabe, M., Nakura, H., Tanaka, M., Kumai, T., & Kobayashi, S. (1998). Liver damage induced by bile duct ligation affects CYP isoenzymes differently in rats. Pharmacology and Toxicology, 82(2), 89-92.

Liver damage induced by bile duct ligation affects CYP isoenzymes differently in rats. / Tateishi, Tomonori; Watanabe, Minoru; Nakura, Hironori; Tanaka, Masami; Kumai, Toshio; Kobayashi, Shinichi.

In: Pharmacology and Toxicology, Vol. 82, No. 2, 1998, p. 89-92.

Research output: Contribution to journalArticle

Tateishi, T, Watanabe, M, Nakura, H, Tanaka, M, Kumai, T & Kobayashi, S 1998, 'Liver damage induced by bile duct ligation affects CYP isoenzymes differently in rats', Pharmacology and Toxicology, vol. 82, no. 2, pp. 89-92.
Tateishi, Tomonori ; Watanabe, Minoru ; Nakura, Hironori ; Tanaka, Masami ; Kumai, Toshio ; Kobayashi, Shinichi. / Liver damage induced by bile duct ligation affects CYP isoenzymes differently in rats. In: Pharmacology and Toxicology. 1998 ; Vol. 82, No. 2. pp. 89-92.
@article{62ac93b5fca84ff38a5850a3e03e893c,
title = "Liver damage induced by bile duct ligation affects CYP isoenzymes differently in rats",
abstract = "We examined the influence of liver damage induced by bile duct ligation on the activity and the expression of hepatic cytochrome P450 (CYP) 1A, 2B, 2C6, 2C11, 2E1 and 3A2 in male Sprague-Dawley rats. In the ligation group, testosterone 2α-, 16α-, and 6β-hydroxylase activities were severely decreased, whereas ethoxyresorufin O-deethylase and progesterone 21-hydroxylase activities relatively remained. Pentoxyresorufin O-deethylase and chlorzoxazone 6-hydroxylase activities were reduced to approximately one thirds those of control. The protein contents of these isoenzymes expressed in hepatic microsomes of the ligation group were decreased to 45{\%}, 32{\%}, 79{\%}, 13{\%}, 58{\%}, and 23{\%} of control for CYP1A, 2B, 2C6, 2C11, 2E1 and 3A2, respectively. The rank order of magnitude of the influence of bile duct ligation on CYP isoenzymes, assessed by the reduction in the enzyme activity and the protein content, corresponded with each other except CYP1A. The reduction of the enzyme activities significantly correlated with the reduction in the protein contents of different isoenzymes. These results suggested that bile duct ligation affected CYP isoenzyme activities and contents with different extent.",
author = "Tomonori Tateishi and Minoru Watanabe and Hironori Nakura and Masami Tanaka and Toshio Kumai and Shinichi Kobayashi",
year = "1998",
language = "English",
volume = "82",
pages = "89--92",
journal = "Basic and Clinical Pharmacology and Toxicology",
issn = "1742-7835",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Liver damage induced by bile duct ligation affects CYP isoenzymes differently in rats

AU - Tateishi, Tomonori

AU - Watanabe, Minoru

AU - Nakura, Hironori

AU - Tanaka, Masami

AU - Kumai, Toshio

AU - Kobayashi, Shinichi

PY - 1998

Y1 - 1998

N2 - We examined the influence of liver damage induced by bile duct ligation on the activity and the expression of hepatic cytochrome P450 (CYP) 1A, 2B, 2C6, 2C11, 2E1 and 3A2 in male Sprague-Dawley rats. In the ligation group, testosterone 2α-, 16α-, and 6β-hydroxylase activities were severely decreased, whereas ethoxyresorufin O-deethylase and progesterone 21-hydroxylase activities relatively remained. Pentoxyresorufin O-deethylase and chlorzoxazone 6-hydroxylase activities were reduced to approximately one thirds those of control. The protein contents of these isoenzymes expressed in hepatic microsomes of the ligation group were decreased to 45%, 32%, 79%, 13%, 58%, and 23% of control for CYP1A, 2B, 2C6, 2C11, 2E1 and 3A2, respectively. The rank order of magnitude of the influence of bile duct ligation on CYP isoenzymes, assessed by the reduction in the enzyme activity and the protein content, corresponded with each other except CYP1A. The reduction of the enzyme activities significantly correlated with the reduction in the protein contents of different isoenzymes. These results suggested that bile duct ligation affected CYP isoenzyme activities and contents with different extent.

AB - We examined the influence of liver damage induced by bile duct ligation on the activity and the expression of hepatic cytochrome P450 (CYP) 1A, 2B, 2C6, 2C11, 2E1 and 3A2 in male Sprague-Dawley rats. In the ligation group, testosterone 2α-, 16α-, and 6β-hydroxylase activities were severely decreased, whereas ethoxyresorufin O-deethylase and progesterone 21-hydroxylase activities relatively remained. Pentoxyresorufin O-deethylase and chlorzoxazone 6-hydroxylase activities were reduced to approximately one thirds those of control. The protein contents of these isoenzymes expressed in hepatic microsomes of the ligation group were decreased to 45%, 32%, 79%, 13%, 58%, and 23% of control for CYP1A, 2B, 2C6, 2C11, 2E1 and 3A2, respectively. The rank order of magnitude of the influence of bile duct ligation on CYP isoenzymes, assessed by the reduction in the enzyme activity and the protein content, corresponded with each other except CYP1A. The reduction of the enzyme activities significantly correlated with the reduction in the protein contents of different isoenzymes. These results suggested that bile duct ligation affected CYP isoenzyme activities and contents with different extent.

UR - http://www.scopus.com/inward/record.url?scp=0031882873&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031882873&partnerID=8YFLogxK

M3 - Article

VL - 82

SP - 89

EP - 92

JO - Basic and Clinical Pharmacology and Toxicology

JF - Basic and Clinical Pharmacology and Toxicology

SN - 1742-7835

IS - 2

ER -