Lithium toxicity and Na+ (Li+)/H+ antiporter in Escherichia coli

K. Inaba, T. Kuroda, T. Shimamoto, T. Kayahara, M. Tsuda, T. Tsuchiya

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The lithium ion (Li+) shows toxicity against Escherichia coli cells when present in a high concentration in the environment. Since Li+ is extruded from cells via a Na+ (Li+)/H+ antiporter, this antiporter must be involved in the detoxification of Li+. Two Na+ (Li+)/H+ antiporters (NhaA system and NhaB system) are known to be present in E. coli. We investigated the properties of the antiporters and the participation of these systems in the detoxification of Li+ using mutants lacking one of the antiporters, or lacking both of them. Although the affinity for Li+ of the two systems was almost the same, the V(max) value for Li+ transport of the NhaA system was about 12 times larger than that of the NhaB system. Wild type cells were unable to grow in the presence of 0.7 M LiCl. Although a wild type cell and a mutant lacking the NhaB system grew in the presence of 0.6 M LiCl, a mutant lacking the NhaA system did not. This second mutant grew in the presence of 0.1 to 0.2 M LiCl. A mutant lacking both the NhaA and NhaB systems could not grow in the presence of 30 mM LiCl.

Original languageEnglish
Pages (from-to)395-398
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume17
Issue number3
Publication statusPublished - 1994

Fingerprint

Sodium-Hydrogen Antiporter
Antiporters
Lithium
Escherichia coli
Ions
sodium-lithium countertransporter

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Inaba, K., Kuroda, T., Shimamoto, T., Kayahara, T., Tsuda, M., & Tsuchiya, T. (1994). Lithium toxicity and Na+ (Li+)/H+ antiporter in Escherichia coli. Biological and Pharmaceutical Bulletin, 17(3), 395-398.

Lithium toxicity and Na+ (Li+)/H+ antiporter in Escherichia coli. / Inaba, K.; Kuroda, T.; Shimamoto, T.; Kayahara, T.; Tsuda, M.; Tsuchiya, T.

In: Biological and Pharmaceutical Bulletin, Vol. 17, No. 3, 1994, p. 395-398.

Research output: Contribution to journalArticle

Inaba, K, Kuroda, T, Shimamoto, T, Kayahara, T, Tsuda, M & Tsuchiya, T 1994, 'Lithium toxicity and Na+ (Li+)/H+ antiporter in Escherichia coli', Biological and Pharmaceutical Bulletin, vol. 17, no. 3, pp. 395-398.
Inaba K, Kuroda T, Shimamoto T, Kayahara T, Tsuda M, Tsuchiya T. Lithium toxicity and Na+ (Li+)/H+ antiporter in Escherichia coli. Biological and Pharmaceutical Bulletin. 1994;17(3):395-398.
Inaba, K. ; Kuroda, T. ; Shimamoto, T. ; Kayahara, T. ; Tsuda, M. ; Tsuchiya, T. / Lithium toxicity and Na+ (Li+)/H+ antiporter in Escherichia coli. In: Biological and Pharmaceutical Bulletin. 1994 ; Vol. 17, No. 3. pp. 395-398.
@article{9d01b030e63f4f2cab5521fc940f72e1,
title = "Lithium toxicity and Na+ (Li+)/H+ antiporter in Escherichia coli",
abstract = "The lithium ion (Li+) shows toxicity against Escherichia coli cells when present in a high concentration in the environment. Since Li+ is extruded from cells via a Na+ (Li+)/H+ antiporter, this antiporter must be involved in the detoxification of Li+. Two Na+ (Li+)/H+ antiporters (NhaA system and NhaB system) are known to be present in E. coli. We investigated the properties of the antiporters and the participation of these systems in the detoxification of Li+ using mutants lacking one of the antiporters, or lacking both of them. Although the affinity for Li+ of the two systems was almost the same, the V(max) value for Li+ transport of the NhaA system was about 12 times larger than that of the NhaB system. Wild type cells were unable to grow in the presence of 0.7 M LiCl. Although a wild type cell and a mutant lacking the NhaB system grew in the presence of 0.6 M LiCl, a mutant lacking the NhaA system did not. This second mutant grew in the presence of 0.1 to 0.2 M LiCl. A mutant lacking both the NhaA and NhaB systems could not grow in the presence of 30 mM LiCl.",
author = "K. Inaba and T. Kuroda and T. Shimamoto and T. Kayahara and M. Tsuda and T. Tsuchiya",
year = "1994",
language = "English",
volume = "17",
pages = "395--398",
journal = "Biological and Pharmaceutical Bulletin",
issn = "0918-6158",
publisher = "Pharmaceutical Society of Japan",
number = "3",

}

TY - JOUR

T1 - Lithium toxicity and Na+ (Li+)/H+ antiporter in Escherichia coli

AU - Inaba, K.

AU - Kuroda, T.

AU - Shimamoto, T.

AU - Kayahara, T.

AU - Tsuda, M.

AU - Tsuchiya, T.

PY - 1994

Y1 - 1994

N2 - The lithium ion (Li+) shows toxicity against Escherichia coli cells when present in a high concentration in the environment. Since Li+ is extruded from cells via a Na+ (Li+)/H+ antiporter, this antiporter must be involved in the detoxification of Li+. Two Na+ (Li+)/H+ antiporters (NhaA system and NhaB system) are known to be present in E. coli. We investigated the properties of the antiporters and the participation of these systems in the detoxification of Li+ using mutants lacking one of the antiporters, or lacking both of them. Although the affinity for Li+ of the two systems was almost the same, the V(max) value for Li+ transport of the NhaA system was about 12 times larger than that of the NhaB system. Wild type cells were unable to grow in the presence of 0.7 M LiCl. Although a wild type cell and a mutant lacking the NhaB system grew in the presence of 0.6 M LiCl, a mutant lacking the NhaA system did not. This second mutant grew in the presence of 0.1 to 0.2 M LiCl. A mutant lacking both the NhaA and NhaB systems could not grow in the presence of 30 mM LiCl.

AB - The lithium ion (Li+) shows toxicity against Escherichia coli cells when present in a high concentration in the environment. Since Li+ is extruded from cells via a Na+ (Li+)/H+ antiporter, this antiporter must be involved in the detoxification of Li+. Two Na+ (Li+)/H+ antiporters (NhaA system and NhaB system) are known to be present in E. coli. We investigated the properties of the antiporters and the participation of these systems in the detoxification of Li+ using mutants lacking one of the antiporters, or lacking both of them. Although the affinity for Li+ of the two systems was almost the same, the V(max) value for Li+ transport of the NhaA system was about 12 times larger than that of the NhaB system. Wild type cells were unable to grow in the presence of 0.7 M LiCl. Although a wild type cell and a mutant lacking the NhaB system grew in the presence of 0.6 M LiCl, a mutant lacking the NhaA system did not. This second mutant grew in the presence of 0.1 to 0.2 M LiCl. A mutant lacking both the NhaA and NhaB systems could not grow in the presence of 30 mM LiCl.

UR - http://www.scopus.com/inward/record.url?scp=0028225729&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028225729&partnerID=8YFLogxK

M3 - Article

C2 - 8019504

AN - SCOPUS:0028225729

VL - 17

SP - 395

EP - 398

JO - Biological and Pharmaceutical Bulletin

JF - Biological and Pharmaceutical Bulletin

SN - 0918-6158

IS - 3

ER -