TY - JOUR
T1 - Lithium chloride inhibits thrombin-induced intracellular calcium mobilization in C6 rat glioma cells
AU - Kagaya, Ariyuki
AU - Okada, Akiko
AU - Tawara, Yasutaka
AU - Inagaki, Masatoshi
AU - Jitsuiki, Hiroaki
AU - Kozuru, Toshiro
AU - Miyoshi, Izuru
AU - Katagiri, Hideaki
AU - Uchitomi, Yosuke
AU - Horiguchi, Jun
AU - Nakata, Yoshihiro
AU - Yamawaki, Shigeto
PY - 2000/1/1
Y1 - 2000/1/1
N2 - 1. In this study, the authors have demonstrated the effect of lithium, a typical mood stabilizer, on thrombin-evoked Ca2+ mobilization in C6 cells to elucidate the action mechanisms of the drug. 2. Thrombin-induced Ca2+ mobilization was reduced 24 hr after 1 or 10 mM lithium chloride (LiCl) pretreatment. The Ca2+ rise was reduced in a time-dependent manner, and the significant inhibition was observed 9 hr pretreatment with 10 mM LiCl. 3. On the other hand, pretreatment of the cells with 10 mM LiCl for 24 hr did not alter the amount of Gαq/11 significantly. Pretreatment with 10 mM LiCl for 24 hr failed to reduce the 5-HT-induced Ca2+ mobilization or to affect the desensitization of the 5-HT signal. 4. Finally, thrombin-elicited Ca2+ rise was markedly inhibited in the presence of 0.05 U/ml plasmin, however, the Ca2+ rise was not further attenuated in the presence of plasmin in C6 cells pretreated with LiCl for 24 hr. 5. These results indicate that pretreatment with LiCl attenuated thrombin-evoked intracellular Ca2+ mobilization in plasmin sensitive manner in C6 rat glioma cells. 6. Thus, it is important to investigate the effect of lithium on thrombin-induced cellular responses to clarify the action mechanism of lithium in relation to some abnormality in thrombin-evoked Ca2+ rise observed in bipolar disorders.
AB - 1. In this study, the authors have demonstrated the effect of lithium, a typical mood stabilizer, on thrombin-evoked Ca2+ mobilization in C6 cells to elucidate the action mechanisms of the drug. 2. Thrombin-induced Ca2+ mobilization was reduced 24 hr after 1 or 10 mM lithium chloride (LiCl) pretreatment. The Ca2+ rise was reduced in a time-dependent manner, and the significant inhibition was observed 9 hr pretreatment with 10 mM LiCl. 3. On the other hand, pretreatment of the cells with 10 mM LiCl for 24 hr did not alter the amount of Gαq/11 significantly. Pretreatment with 10 mM LiCl for 24 hr failed to reduce the 5-HT-induced Ca2+ mobilization or to affect the desensitization of the 5-HT signal. 4. Finally, thrombin-elicited Ca2+ rise was markedly inhibited in the presence of 0.05 U/ml plasmin, however, the Ca2+ rise was not further attenuated in the presence of plasmin in C6 cells pretreated with LiCl for 24 hr. 5. These results indicate that pretreatment with LiCl attenuated thrombin-evoked intracellular Ca2+ mobilization in plasmin sensitive manner in C6 rat glioma cells. 6. Thus, it is important to investigate the effect of lithium on thrombin-induced cellular responses to clarify the action mechanism of lithium in relation to some abnormality in thrombin-evoked Ca2+ rise observed in bipolar disorders.
KW - C6 cells
KW - Calcium
KW - G protein
KW - Lithium
KW - Thrombin
UR - http://www.scopus.com/inward/record.url?scp=0033970428&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033970428&partnerID=8YFLogxK
U2 - 10.1016/S0278-5846(99)00082-2
DO - 10.1016/S0278-5846(99)00082-2
M3 - Article
C2 - 10659985
AN - SCOPUS:0033970428
VL - 24
SP - 85
EP - 95
JO - Progress in Neuro-Psychopharmacology and Biological Psychiatry
JF - Progress in Neuro-Psychopharmacology and Biological Psychiatry
SN - 0278-5846
IS - 1
ER -