Lithium chloride inhibits thrombin-induced intracellular calcium mobilization in C6 rat glioma cells

Ariyuki Kagaya, Akiko Okada, Yasutaka Tawara, Masatoshi Inagaki, Hiroaki Jitsuiki, Toshiro Kozuru, Izuru Miyoshi, Hideaki Katagiri, Yosuke Uchitomi, Jun Horiguchi, Yoshihiro Nakata, Shigeto Yamawaki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

1. In this study, the authors have demonstrated the effect of lithium, a typical mood stabilizer, on thrombin-evoked Ca2+ mobilization in C6 cells to elucidate the action mechanisms of the drug. 2. Thrombin-induced Ca2+ mobilization was reduced 24 hr after 1 or 10 mM lithium chloride (LiCl) pretreatment. The Ca2+ rise was reduced in a time-dependent manner, and the significant inhibition was observed 9 hr pretreatment with 10 mM LiCl. 3. On the other hand, pretreatment of the cells with 10 mM LiCl for 24 hr did not alter the amount of Gαq/11 significantly. Pretreatment with 10 mM LiCl for 24 hr failed to reduce the 5-HT-induced Ca2+ mobilization or to affect the desensitization of the 5-HT signal. 4. Finally, thrombin-elicited Ca2+ rise was markedly inhibited in the presence of 0.05 U/ml plasmin, however, the Ca2+ rise was not further attenuated in the presence of plasmin in C6 cells pretreated with LiCl for 24 hr. 5. These results indicate that pretreatment with LiCl attenuated thrombin-evoked intracellular Ca2+ mobilization in plasmin sensitive manner in C6 rat glioma cells. 6. Thus, it is important to investigate the effect of lithium on thrombin-induced cellular responses to clarify the action mechanism of lithium in relation to some abnormality in thrombin-evoked Ca2+ rise observed in bipolar disorders.

Original languageEnglish
Pages (from-to)85-95
Number of pages11
JournalProgress in Neuro-Psychopharmacology and Biological Psychiatry
Volume24
Issue number1
DOIs
Publication statusPublished - Jan 1 2000

Keywords

  • C6 cells
  • Calcium
  • G protein
  • Lithium
  • Thrombin

ASJC Scopus subject areas

  • Pharmacology
  • Biological Psychiatry

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  • Cite this

    Kagaya, A., Okada, A., Tawara, Y., Inagaki, M., Jitsuiki, H., Kozuru, T., Miyoshi, I., Katagiri, H., Uchitomi, Y., Horiguchi, J., Nakata, Y., & Yamawaki, S. (2000). Lithium chloride inhibits thrombin-induced intracellular calcium mobilization in C6 rat glioma cells. Progress in Neuro-Psychopharmacology and Biological Psychiatry, 24(1), 85-95. https://doi.org/10.1016/S0278-5846(99)00082-2