Abstract
The conditions to increase the sensitivities of liposomes to odorants were examined. The results obtained are as follows. (1) The minimum concentration of amyl acetate to induce the membrane potential changes (threshold) in phosphatidylcholine (PC) liposomes was about 10-4 M and addition of 10 or 20% phospatidylserine (PS) lowered the threshold to about 10-9 M, which was lower than the thresholds for amyl acetate in the turtle and frog olfactory systems. (2) Similar to amyl acetate, addition of PS to PC greatly lowered the threshold for β-ionone. On the other hand, addition of PS to PC in certain ratio increased the threshold for citral, suggesting that addition of PS to PC does not always increase the responses to all odorants. (3) The membrane fluidity change of the liposomes in response to odorants occurred at similar concentration region where the membrane potential changes occurred. The presence of CaCl2 in external solution much greatly increased both the magnitude of the membrane potential changes and the membrane fluidity changes of the PC-PS liposomes in response to amyl acetate than the presence of NaCl and MgCl2. These results suggest that the membrane fluidity change is related to generation of the membrane potential change. (4) It was estimated that adsorption of less than a few molecules of amyl acetate on single liposome elicits detectable changes in the membrane potential and the membrane fluidity.
| Original language | English |
|---|---|
| Pages (from-to) | 7-12 |
| Number of pages | 6 |
| Journal | BBA - Biomembranes |
| Volume | 1062 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Feb 11 1991 |
| Externally published | Yes |
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Keywords
- Liposome
- Membrane fluidity
- Membrane potential
- Odorant
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
Cite this
Liposomes having high sensitivity to odorants. / Enomoto, Shuichi; Kashiwayanagi, Makoto; Kurihara, Kenzo.
In: BBA - Biomembranes, Vol. 1062, No. 1, 11.02.1991, p. 7-12.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Liposomes having high sensitivity to odorants
AU - Enomoto, Shuichi
AU - Kashiwayanagi, Makoto
AU - Kurihara, Kenzo
PY - 1991/2/11
Y1 - 1991/2/11
N2 - The conditions to increase the sensitivities of liposomes to odorants were examined. The results obtained are as follows. (1) The minimum concentration of amyl acetate to induce the membrane potential changes (threshold) in phosphatidylcholine (PC) liposomes was about 10-4 M and addition of 10 or 20% phospatidylserine (PS) lowered the threshold to about 10-9 M, which was lower than the thresholds for amyl acetate in the turtle and frog olfactory systems. (2) Similar to amyl acetate, addition of PS to PC greatly lowered the threshold for β-ionone. On the other hand, addition of PS to PC in certain ratio increased the threshold for citral, suggesting that addition of PS to PC does not always increase the responses to all odorants. (3) The membrane fluidity change of the liposomes in response to odorants occurred at similar concentration region where the membrane potential changes occurred. The presence of CaCl2 in external solution much greatly increased both the magnitude of the membrane potential changes and the membrane fluidity changes of the PC-PS liposomes in response to amyl acetate than the presence of NaCl and MgCl2. These results suggest that the membrane fluidity change is related to generation of the membrane potential change. (4) It was estimated that adsorption of less than a few molecules of amyl acetate on single liposome elicits detectable changes in the membrane potential and the membrane fluidity.
AB - The conditions to increase the sensitivities of liposomes to odorants were examined. The results obtained are as follows. (1) The minimum concentration of amyl acetate to induce the membrane potential changes (threshold) in phosphatidylcholine (PC) liposomes was about 10-4 M and addition of 10 or 20% phospatidylserine (PS) lowered the threshold to about 10-9 M, which was lower than the thresholds for amyl acetate in the turtle and frog olfactory systems. (2) Similar to amyl acetate, addition of PS to PC greatly lowered the threshold for β-ionone. On the other hand, addition of PS to PC in certain ratio increased the threshold for citral, suggesting that addition of PS to PC does not always increase the responses to all odorants. (3) The membrane fluidity change of the liposomes in response to odorants occurred at similar concentration region where the membrane potential changes occurred. The presence of CaCl2 in external solution much greatly increased both the magnitude of the membrane potential changes and the membrane fluidity changes of the PC-PS liposomes in response to amyl acetate than the presence of NaCl and MgCl2. These results suggest that the membrane fluidity change is related to generation of the membrane potential change. (4) It was estimated that adsorption of less than a few molecules of amyl acetate on single liposome elicits detectable changes in the membrane potential and the membrane fluidity.
KW - Liposome
KW - Membrane fluidity
KW - Membrane potential
KW - Odorant
UR - http://www.scopus.com/inward/record.url?scp=0026012770&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026012770&partnerID=8YFLogxK
U2 - 10.1016/0005-2736(91)90327-5
DO - 10.1016/0005-2736(91)90327-5
M3 - Article
C2 - 1998711
AN - SCOPUS:0026012770
VL - 1062
SP - 7
EP - 12
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
SN - 0005-2736
IS - 1
ER -