Liposomes destabilize tRNA during heat stress

Keishi Suga, Hiroshi Umakoshi, Hibiki Tomita, Tomoyuki Tanabe, Toshinori Shimanouchi, Ryoichi Kuboi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Biomembranes play an important role in cellular response to heat stress. In this study, we focus on the interaction between liposomes and tRNA. Upon heat treatment we determined circular dichroism spectra of tRNA in presence of liposomes prepared from POPC (1-palmitoyl-2-oleoylsn-glycero-3-phosphocholine) and cholesterol (Ch). To compare thermal stability, midpoint temperature (T m) of tRNA was calculated from normalized θ208. Addition of POPC/Ch liposomes decreased the Tm value of tRNA from 48°C to 38°C. We conclude that POPC/Ch liposomes interact with tRNA and destabilize its conformation under heat stress.

Original languageEnglish
Pages (from-to)526-529
Number of pages4
JournalBiotechnology Journal
Volume5
Issue number5
DOIs
Publication statusPublished - May 2010

Keywords

  • Biochemical engineering
  • Heat stress response
  • Liposome membrane stress biotechnology
  • tRNA conformation

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Molecular Medicine

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    Suga, K., Umakoshi, H., Tomita, H., Tanabe, T., Shimanouchi, T., & Kuboi, R. (2010). Liposomes destabilize tRNA during heat stress. Biotechnology Journal, 5(5), 526-529. https://doi.org/10.1002/biot.200900289