Liposome membrane itself can affect gene expression in the Escherichia coli cell-free translation system

Huong Thi Bui, Hiroshi Umakoshi, Kien Xuan Ngo, Masato Nishida, Toshinori Shimanouchi, Ryoichi Kuboi

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

A possible role of a model biomembrane, liposome, in gene expression was investigated by using the cell-free translation system. A reporter protein, green fluorescence protein (GFT), was expressed in vitro with and without liposome prepared with l-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidyl chorine (POPC) and cholesterol (Ch) (5.7 niM lipid concentration). In the presence of POPC/Ch liposome, the fluorescence intensity of produced GFP was found to be 1.67 times higher than that in the control after 18 h of expression. The results of the SDS-PAGE analysis also show the above promotion effect of the liposome on the net expression of the GFP gene (1.58 times more). The amounts of mRNA were found to be promoted to 1.29 times higher than those in the control. The differences among mRNA, net expression of the GFP gene, and GFP fluorescence indicate that the enhanced GFP expression in the presence of POPC/Ch liposome could primarily affect the transcription and translation of the GFP gene among the possible steps of gene expression. The variation of in vitro gene expression with various liposomes also shows that the biomembrane could act as a modulator to split the genotype and phenotype in a biological cell.

Original languageEnglish
Pages (from-to)10537-10542
Number of pages6
JournalLangmuir
Volume24
Issue number19
DOIs
Publication statusPublished - Oct 7 2008
Externally publishedYes

Fingerprint

Liposomes
gene expression
Escherichia
Gene expression
Escherichia coli
cholesterol
genes
membranes
Membranes
Cholesterol
cells
fluorescence
Genes
Fluorescence
proteins
phenotype
promotion
Proteins
lipids
modulators

ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy

Cite this

Liposome membrane itself can affect gene expression in the Escherichia coli cell-free translation system. / Bui, Huong Thi; Umakoshi, Hiroshi; Ngo, Kien Xuan; Nishida, Masato; Shimanouchi, Toshinori; Kuboi, Ryoichi.

In: Langmuir, Vol. 24, No. 19, 07.10.2008, p. 10537-10542.

Research output: Contribution to journalArticle

Bui, Huong Thi ; Umakoshi, Hiroshi ; Ngo, Kien Xuan ; Nishida, Masato ; Shimanouchi, Toshinori ; Kuboi, Ryoichi. / Liposome membrane itself can affect gene expression in the Escherichia coli cell-free translation system. In: Langmuir. 2008 ; Vol. 24, No. 19. pp. 10537-10542.
@article{7c3cb553c4e049ed95300d45b33b9820,
title = "Liposome membrane itself can affect gene expression in the Escherichia coli cell-free translation system",
abstract = "A possible role of a model biomembrane, liposome, in gene expression was investigated by using the cell-free translation system. A reporter protein, green fluorescence protein (GFT), was expressed in vitro with and without liposome prepared with l-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidyl chorine (POPC) and cholesterol (Ch) (5.7 niM lipid concentration). In the presence of POPC/Ch liposome, the fluorescence intensity of produced GFP was found to be 1.67 times higher than that in the control after 18 h of expression. The results of the SDS-PAGE analysis also show the above promotion effect of the liposome on the net expression of the GFP gene (1.58 times more). The amounts of mRNA were found to be promoted to 1.29 times higher than those in the control. The differences among mRNA, net expression of the GFP gene, and GFP fluorescence indicate that the enhanced GFP expression in the presence of POPC/Ch liposome could primarily affect the transcription and translation of the GFP gene among the possible steps of gene expression. The variation of in vitro gene expression with various liposomes also shows that the biomembrane could act as a modulator to split the genotype and phenotype in a biological cell.",
author = "Bui, {Huong Thi} and Hiroshi Umakoshi and Ngo, {Kien Xuan} and Masato Nishida and Toshinori Shimanouchi and Ryoichi Kuboi",
year = "2008",
month = "10",
day = "7",
doi = "10.1021/la801962j",
language = "English",
volume = "24",
pages = "10537--10542",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "19",

}

TY - JOUR

T1 - Liposome membrane itself can affect gene expression in the Escherichia coli cell-free translation system

AU - Bui, Huong Thi

AU - Umakoshi, Hiroshi

AU - Ngo, Kien Xuan

AU - Nishida, Masato

AU - Shimanouchi, Toshinori

AU - Kuboi, Ryoichi

PY - 2008/10/7

Y1 - 2008/10/7

N2 - A possible role of a model biomembrane, liposome, in gene expression was investigated by using the cell-free translation system. A reporter protein, green fluorescence protein (GFT), was expressed in vitro with and without liposome prepared with l-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidyl chorine (POPC) and cholesterol (Ch) (5.7 niM lipid concentration). In the presence of POPC/Ch liposome, the fluorescence intensity of produced GFP was found to be 1.67 times higher than that in the control after 18 h of expression. The results of the SDS-PAGE analysis also show the above promotion effect of the liposome on the net expression of the GFP gene (1.58 times more). The amounts of mRNA were found to be promoted to 1.29 times higher than those in the control. The differences among mRNA, net expression of the GFP gene, and GFP fluorescence indicate that the enhanced GFP expression in the presence of POPC/Ch liposome could primarily affect the transcription and translation of the GFP gene among the possible steps of gene expression. The variation of in vitro gene expression with various liposomes also shows that the biomembrane could act as a modulator to split the genotype and phenotype in a biological cell.

AB - A possible role of a model biomembrane, liposome, in gene expression was investigated by using the cell-free translation system. A reporter protein, green fluorescence protein (GFT), was expressed in vitro with and without liposome prepared with l-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidyl chorine (POPC) and cholesterol (Ch) (5.7 niM lipid concentration). In the presence of POPC/Ch liposome, the fluorescence intensity of produced GFP was found to be 1.67 times higher than that in the control after 18 h of expression. The results of the SDS-PAGE analysis also show the above promotion effect of the liposome on the net expression of the GFP gene (1.58 times more). The amounts of mRNA were found to be promoted to 1.29 times higher than those in the control. The differences among mRNA, net expression of the GFP gene, and GFP fluorescence indicate that the enhanced GFP expression in the presence of POPC/Ch liposome could primarily affect the transcription and translation of the GFP gene among the possible steps of gene expression. The variation of in vitro gene expression with various liposomes also shows that the biomembrane could act as a modulator to split the genotype and phenotype in a biological cell.

UR - http://www.scopus.com/inward/record.url?scp=54549123529&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=54549123529&partnerID=8YFLogxK

U2 - 10.1021/la801962j

DO - 10.1021/la801962j

M3 - Article

C2 - 18759465

AN - SCOPUS:54549123529

VL - 24

SP - 10537

EP - 10542

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 19

ER -