Lipopolysaccharides, cytokines, and nitric oxide affect secretion of prostaglandins and leukotrienes by bovine mammary gland epithelial cells

K. K. Piotrowska-Tomala, M. J. Siemieniuch, A. Z. Szóstek, A. J. Korzekwa, I. Woclawek-Potocka, A. M. Galváo, K. Okuda, D. J. Skarzynski

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Abstract

The aims of this study were to determine the effects of lipopolysaccharides (LPS), tumor necrosis factor (TNF), interleukin 1 alpha (IL-1α), nitric oxide donor (NONOate), or the combination of TNF α IL-1α α NONOate on the following: (i) secretion of prostaglandin (PG)-F2α, PGE2, leukotriene (LT)-B4, and LTC4 by epithelial cells of the teat cavity and lactiferous sinus of bovine mammary gland; (ii) messenger RNA (mRNA) transcription of enzymes responsible for arachidonic acid (AA) metabolism (prostaglandin- endoperoxide synthase 2 [PTGS2], prostaglandin E synthase [PTGES], prostaglandin F synthase [PGFS], and arachidonate 5-lipooxygenase [ALOX5]); and (iii) proliferation of the cells. The cells were stimulated for 24 h. Prostaglandins and LT were measured by enzyme immunoassay, mRNA transcription of enzymes was determined by real-time reverse transcription polymerase chain reaction, and the cell viability was measured by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide. All factors increased PG secretion, but the highest stimulation was observed after TNF and IL-1α (P<0.001). Tumor necrosis factor, NONOate, and TNF α IL-1α α NONOate increased LTB4 production (P<0.01), whereas LTC4 was increased by LPS, TNF, and IL-1α (P<0.01). Lipopolysaccharides, TNF, IL-1α, and the reagents combination increased PTGS2, PTGES, and PGFS mRNA transcription (P0.01), whereas ALOX5 mRNA transcription was increased only by TNF (P

Original languageEnglish
Pages (from-to)278-288
Number of pages11
JournalDomestic Animal Endocrinology
Volume43
Issue number4
DOIs
Publication statusPublished - Nov 2012

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ASJC Scopus subject areas

  • Animal Science and Zoology
  • Endocrinology
  • Food Animals

Cite this

Piotrowska-Tomala, K. K., Siemieniuch, M. J., Szóstek, A. Z., Korzekwa, A. J., Woclawek-Potocka, I., Galváo, A. M., Okuda, K., & Skarzynski, D. J. (2012). Lipopolysaccharides, cytokines, and nitric oxide affect secretion of prostaglandins and leukotrienes by bovine mammary gland epithelial cells. Domestic Animal Endocrinology, 43(4), 278-288. https://doi.org/10.1016/j.domaniend.2012.04.005