Lipopolysaccharide-stimulated transglutaminase 2 expression enhances endocytosis activity in the mouse microglial cell line BV-2

Kenji Kawabe, Katsura Takano, Mitsuaki Moriyama, Yoichi Nakamura

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Objectives: In peripheral macrophages, tissue-type transglutaminase (TG2) is reported to be involved in phagocytosis of apoptotic cells. However, the contribution of TG2 to microglial phagocytosis has not been investigated. In this study, using a microglial cell line, BV-2, we examined the changes in TG2 expression, phagocytosis and pinocytosis in cells stimulated by lipopolysaccharide (LPS). Methods: Cells of the mouse microglial cell line BV-2 were stimulated by LPS with or without cystamine, an inhibitor of TG enzyme activity, for 24 h. TG2 expression was measured by real-time RT-PCR and Western blotting. TG activity was evaluated using biotinylated pentylamine as a substrate. Pinocytosis was determined by uptake of 1-μm fluorescent microbeads. Phagocytosis was assessed by uptake of dead cells, human neuroblastoma SH-SY5Y cells, which were pretreated with H2O2 for 24 h. Results: Phagocytosis of dead cells and pinocytosis of fluorescent microbeads were up-regulated by LPS stimulation together with TG2 expression. Blockade of TG enzyme activity by cystamine suppressed TG2 expression, phagocytosis and pinocytosis. Conclusions: These results suggested that LPS-induced TG2 was involved in the mechanism of pinocytosis and phagocytosis in microglia.

Original languageEnglish
Pages (from-to)243-249
Number of pages7
JournalNeuroImmunoModulation
Volume22
Issue number4
DOIs
Publication statusPublished - Apr 24 2015
Externally publishedYes

Fingerprint

Pinocytosis
Endocytosis
Phagocytosis
Lipopolysaccharides
Cell Line
Cystamine
Cytophagocytosis
Microspheres
Microglia
Enzyme Inhibitors
Neuroblastoma
Real-Time Polymerase Chain Reaction
Western Blotting
Macrophages
transglutaminase 2
Enzymes

Keywords

  • Cystamine
  • Endocytosis
  • Microglia
  • Phagocytosis
  • Pinocytosis
  • Transglutaminase 2

ASJC Scopus subject areas

  • Endocrinology
  • Immunology
  • Endocrine and Autonomic Systems
  • Neurology
  • Medicine(all)

Cite this

Lipopolysaccharide-stimulated transglutaminase 2 expression enhances endocytosis activity in the mouse microglial cell line BV-2. / Kawabe, Kenji; Takano, Katsura; Moriyama, Mitsuaki; Nakamura, Yoichi.

In: NeuroImmunoModulation, Vol. 22, No. 4, 24.04.2015, p. 243-249.

Research output: Contribution to journalArticle

Kawabe, Kenji ; Takano, Katsura ; Moriyama, Mitsuaki ; Nakamura, Yoichi. / Lipopolysaccharide-stimulated transglutaminase 2 expression enhances endocytosis activity in the mouse microglial cell line BV-2. In: NeuroImmunoModulation. 2015 ; Vol. 22, No. 4. pp. 243-249.
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